Conjunctival epithelium expression of HLA-DR in dry eye patients

Kazuo Tsubota, Tsutomu Fujihara, Keiko Saito, Tsutomu Takeuchi

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Purpose: To compare conjunctival epithelium expression of HLA-DR and ICAM-1 with tear dynamics and ocular surface parameters. Methods: Brush cytology and flow cytometry were used to quantitatively analyze HLA-DR and ICAM-1 expression in 28 dry eye patients. Results: HLA-DR was expressed in 66% of the conjunctival cells of dry eye patients. This expression correlates with that of ICAM-1, as well as with the Schirmer test results (p<0.05). Conclusion: This study confirmed that dry eye consists not only of ocular surface desiccation, but also of upregulation of the HLA-DR and ICAM-1 molecule in the conjunctival epithelium, possibly resulting in increased inflammation.

Original languageEnglish
Pages (from-to)16-19
Number of pages4
JournalOphthalmologica
Volume213
Issue number1
DOIs
Publication statusPublished - 1999

Fingerprint

HLA-DR Antigens
Intercellular Adhesion Molecule-1
Epithelium
Desiccation
Tears
Cell Biology
Flow Cytometry
Up-Regulation
Inflammation

Keywords

  • Conjunctival epithelium
  • Dry eye
  • Flow cytometry
  • Fluorescein staining
  • HLA-DR
  • Rose bengal staining
  • Sjogren's syndrome

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Conjunctival epithelium expression of HLA-DR in dry eye patients. / Tsubota, Kazuo; Fujihara, Tsutomu; Saito, Keiko; Takeuchi, Tsutomu.

In: Ophthalmologica, Vol. 213, No. 1, 1999, p. 16-19.

Research output: Contribution to journalArticle

Tsubota, Kazuo ; Fujihara, Tsutomu ; Saito, Keiko ; Takeuchi, Tsutomu. / Conjunctival epithelium expression of HLA-DR in dry eye patients. In: Ophthalmologica. 1999 ; Vol. 213, No. 1. pp. 16-19.
@article{55e6c5bfa8384286be786a5b4ccc4702,
title = "Conjunctival epithelium expression of HLA-DR in dry eye patients",
abstract = "Purpose: To compare conjunctival epithelium expression of HLA-DR and ICAM-1 with tear dynamics and ocular surface parameters. Methods: Brush cytology and flow cytometry were used to quantitatively analyze HLA-DR and ICAM-1 expression in 28 dry eye patients. Results: HLA-DR was expressed in 66{\%} of the conjunctival cells of dry eye patients. This expression correlates with that of ICAM-1, as well as with the Schirmer test results (p<0.05). Conclusion: This study confirmed that dry eye consists not only of ocular surface desiccation, but also of upregulation of the HLA-DR and ICAM-1 molecule in the conjunctival epithelium, possibly resulting in increased inflammation.",
keywords = "Conjunctival epithelium, Dry eye, Flow cytometry, Fluorescein staining, HLA-DR, Rose bengal staining, Sjogren's syndrome",
author = "Kazuo Tsubota and Tsutomu Fujihara and Keiko Saito and Tsutomu Takeuchi",
year = "1999",
doi = "10.1159/000027387",
language = "English",
volume = "213",
pages = "16--19",
journal = "Ophthalmologica",
issn = "0030-3755",
publisher = "S. Karger AG",
number = "1",

}

TY - JOUR

T1 - Conjunctival epithelium expression of HLA-DR in dry eye patients

AU - Tsubota, Kazuo

AU - Fujihara, Tsutomu

AU - Saito, Keiko

AU - Takeuchi, Tsutomu

PY - 1999

Y1 - 1999

N2 - Purpose: To compare conjunctival epithelium expression of HLA-DR and ICAM-1 with tear dynamics and ocular surface parameters. Methods: Brush cytology and flow cytometry were used to quantitatively analyze HLA-DR and ICAM-1 expression in 28 dry eye patients. Results: HLA-DR was expressed in 66% of the conjunctival cells of dry eye patients. This expression correlates with that of ICAM-1, as well as with the Schirmer test results (p<0.05). Conclusion: This study confirmed that dry eye consists not only of ocular surface desiccation, but also of upregulation of the HLA-DR and ICAM-1 molecule in the conjunctival epithelium, possibly resulting in increased inflammation.

AB - Purpose: To compare conjunctival epithelium expression of HLA-DR and ICAM-1 with tear dynamics and ocular surface parameters. Methods: Brush cytology and flow cytometry were used to quantitatively analyze HLA-DR and ICAM-1 expression in 28 dry eye patients. Results: HLA-DR was expressed in 66% of the conjunctival cells of dry eye patients. This expression correlates with that of ICAM-1, as well as with the Schirmer test results (p<0.05). Conclusion: This study confirmed that dry eye consists not only of ocular surface desiccation, but also of upregulation of the HLA-DR and ICAM-1 molecule in the conjunctival epithelium, possibly resulting in increased inflammation.

KW - Conjunctival epithelium

KW - Dry eye

KW - Flow cytometry

KW - Fluorescein staining

KW - HLA-DR

KW - Rose bengal staining

KW - Sjogren's syndrome

UR - http://www.scopus.com/inward/record.url?scp=0032890237&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032890237&partnerID=8YFLogxK

U2 - 10.1159/000027387

DO - 10.1159/000027387

M3 - Article

VL - 213

SP - 16

EP - 19

JO - Ophthalmologica

JF - Ophthalmologica

SN - 0030-3755

IS - 1

ER -