Conjunctival epithelium expression of HLA-DR in dry eye patients

Kazuo Tsubota; Tsutomu Fujihara; Keiko Saito; Tsutomu Takeuchi

doi:10.1159/000027387

Conjunctival epithelium expression of HLA-DR in dry eye patients

Kazuo Tsubota, Tsutomu Fujihara, Keiko Saito, Tsutomu Takeuchi

Department of Ophthalmology

Research output: Contribution to journal › Article › peer-review

95 Citations (Scopus)

Abstract

Purpose: To compare conjunctival epithelium expression of HLA-DR and ICAM-1 with tear dynamics and ocular surface parameters. Methods: Brush cytology and flow cytometry were used to quantitatively analyze HLA-DR and ICAM-1 expression in 28 dry eye patients. Results: HLA-DR was expressed in 66% of the conjunctival cells of dry eye patients. This expression correlates with that of ICAM-1, as well as with the Schirmer test results (p<0.05). Conclusion: This study confirmed that dry eye consists not only of ocular surface desiccation, but also of upregulation of the HLA-DR and ICAM-1 molecule in the conjunctival epithelium, possibly resulting in increased inflammation.

Original language	English
Pages (from-to)	16-19
Number of pages	4
Journal	Ophthalmologica
Volume	213
Issue number	1
DOIs	https://doi.org/10.1159/000027387
Publication status	Published - 1999

Keywords

Conjunctival epithelium
Dry eye
Flow cytometry
Fluorescein staining
HLA-DR
Rose bengal staining
Sjogren's syndrome

ASJC Scopus subject areas

Ophthalmology
Sensory Systems

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10.1159/000027387

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title = "Conjunctival epithelium expression of HLA-DR in dry eye patients",

abstract = "Purpose: To compare conjunctival epithelium expression of HLA-DR and ICAM-1 with tear dynamics and ocular surface parameters. Methods: Brush cytology and flow cytometry were used to quantitatively analyze HLA-DR and ICAM-1 expression in 28 dry eye patients. Results: HLA-DR was expressed in 66% of the conjunctival cells of dry eye patients. This expression correlates with that of ICAM-1, as well as with the Schirmer test results (p<0.05). Conclusion: This study confirmed that dry eye consists not only of ocular surface desiccation, but also of upregulation of the HLA-DR and ICAM-1 molecule in the conjunctival epithelium, possibly resulting in increased inflammation.",

keywords = "Conjunctival epithelium, Dry eye, Flow cytometry, Fluorescein staining, HLA-DR, Rose bengal staining, Sjogren's syndrome",

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year = "1999",

doi = "10.1159/000027387",

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volume = "213",

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journal = "Ophthalmologica",

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T1 - Conjunctival epithelium expression of HLA-DR in dry eye patients

AU - Tsubota, Kazuo

AU - Fujihara, Tsutomu

AU - Saito, Keiko

AU - Takeuchi, Tsutomu

PY - 1999

Y1 - 1999

N2 - Purpose: To compare conjunctival epithelium expression of HLA-DR and ICAM-1 with tear dynamics and ocular surface parameters. Methods: Brush cytology and flow cytometry were used to quantitatively analyze HLA-DR and ICAM-1 expression in 28 dry eye patients. Results: HLA-DR was expressed in 66% of the conjunctival cells of dry eye patients. This expression correlates with that of ICAM-1, as well as with the Schirmer test results (p<0.05). Conclusion: This study confirmed that dry eye consists not only of ocular surface desiccation, but also of upregulation of the HLA-DR and ICAM-1 molecule in the conjunctival epithelium, possibly resulting in increased inflammation.

AB - Purpose: To compare conjunctival epithelium expression of HLA-DR and ICAM-1 with tear dynamics and ocular surface parameters. Methods: Brush cytology and flow cytometry were used to quantitatively analyze HLA-DR and ICAM-1 expression in 28 dry eye patients. Results: HLA-DR was expressed in 66% of the conjunctival cells of dry eye patients. This expression correlates with that of ICAM-1, as well as with the Schirmer test results (p<0.05). Conclusion: This study confirmed that dry eye consists not only of ocular surface desiccation, but also of upregulation of the HLA-DR and ICAM-1 molecule in the conjunctival epithelium, possibly resulting in increased inflammation.

KW - Conjunctival epithelium

KW - Dry eye

KW - Flow cytometry

KW - Fluorescein staining

KW - HLA-DR

KW - Rose bengal staining

KW - Sjogren's syndrome

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Conjunctival epithelium expression of HLA-DR in dry eye patients

Abstract

Keywords

ASJC Scopus subject areas

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