Consequence of the loss of Sox2 in the developing brain of the mouse

Satoru Miyagi, Shinji Masui, Hitoshi Niwa, Tetsuichiro Saito, Takuya Shimazaki, Hideyuki Okano, Masazumi Nishimoto, Masami Muramatsu, Atsushi Iwama, Akihiko Okuda

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

The transcription factor Sox2 is expressed at high levels in neural stem and progenitor cells. Here, we inactivated Sox2 specifically in the developing brain by using Cre-loxP system. Although mutant animals did not survive after birth, analysis of late gestation embryos revealed that loss of Sox2 causes enlargement of the lateral ventricles and a decrease in the number of neurosphere-forming cells. However, although their neurogenic potential is attenuated, Sox2-deficient neural stem cells retain their multipotency and self-renewal capacity. We found that expression level of Sox3 is elevated in Sox2 null developing brain, probably mitigating the effects of loss of Sox2.

Original languageEnglish
Pages (from-to)2811-2815
Number of pages5
JournalFEBS Letters
Volume582
Issue number18
DOIs
Publication statusPublished - 2008 Aug 6

Keywords

  • Conditional knockout
  • Multipotency
  • Neural stem cells
  • Self-renewal
  • Sox2

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Miyagi, S., Masui, S., Niwa, H., Saito, T., Shimazaki, T., Okano, H., Nishimoto, M., Muramatsu, M., Iwama, A., & Okuda, A. (2008). Consequence of the loss of Sox2 in the developing brain of the mouse. FEBS Letters, 582(18), 2811-2815. https://doi.org/10.1016/j.febslet.2008.07.011