Constituents of the Seed of Malva verticillata. III.1) Characterization of the Major Pectic Peptidoglycan and Oligosaccharides

Ryoko Gonda, Masashi Tomoda, Mieko Kanari, Naomi Yoneda, Towako Shimizu, Keiko Shigeshiro, Tomomi Yazawa

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The major pectic peptidoglycan, designated as MVS-V, was isolated from the seeds of Malva verticillata L. It was homogeneous on electrophoresis and gel chromatography. It was composed of about 43% polysaccharide and 57% protein moieties, and its molecular weight was estimated to be 22000. The polysaccharide moiety was isolated, and found to be composed of L-arabinose: d-xylose: D-galactose: L-rhamnose: D-galacturonic acid in the molar ratio of 6: 5 : 3:8 : 24. One-sixth of the D-galacturonic acid residues exist as the methyl esters. 0-Acetyl groups were identified, and the content amounted to 3.3% of the polysaccharide moiety. Methylation analysis of the carboxyl-reduced derivative, nuclear magnetic resonance, periodate oxidation, and partial hydrolysis studies enabled elucidation of the structural features of the polysaccharide moiety. It is a pectic substance having a rhamnogalacturonan backbone with arabinoxylogalactan side chains. Several oligosaccharides belonging to the raffinose family were also isolated and identified from the seeds.

Original languageEnglish
Pages (from-to)2790-2795
Number of pages6
JournalChemical and Pharmaceutical Bulletin
Volume36
Issue number8
DOIs
Publication statusPublished - 1988
Externally publishedYes

Fingerprint

Malva
Peptidoglycan
Oligosaccharides
Polysaccharides
Seed
Seeds
Raffinose
Rhamnose
Arabinose
Methylation
Xylose
Chromatography
Electrophoresis
Galactose
Gel Chromatography
Hydrolysis
Esters
Magnetic Resonance Spectroscopy
Molecular Weight
Gels

Keywords

  • Malva verticillata
  • MVS-V
  • oligosaccharide
  • pectic substance
  • peptidoglycan
  • polysaccharide moiety
  • raffinose family
  • seed
  • structural feature

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

Cite this

Constituents of the Seed of Malva verticillata. III.1) Characterization of the Major Pectic Peptidoglycan and Oligosaccharides. / Gonda, Ryoko; Tomoda, Masashi; Kanari, Mieko; Yoneda, Naomi; Shimizu, Towako; Shigeshiro, Keiko; Yazawa, Tomomi.

In: Chemical and Pharmaceutical Bulletin, Vol. 36, No. 8, 1988, p. 2790-2795.

Research output: Contribution to journalArticle

Gonda, Ryoko ; Tomoda, Masashi ; Kanari, Mieko ; Yoneda, Naomi ; Shimizu, Towako ; Shigeshiro, Keiko ; Yazawa, Tomomi. / Constituents of the Seed of Malva verticillata. III.1) Characterization of the Major Pectic Peptidoglycan and Oligosaccharides. In: Chemical and Pharmaceutical Bulletin. 1988 ; Vol. 36, No. 8. pp. 2790-2795.
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