Constitutive expression of ICAM-1 in rat microvascular systems analyzed by laser confocal microscopy

Yutaka Iigo, Makoto Suematsu, Tomoe Higashida, Jun Ichi Oheda, Keiko Matsumoto, Yoshiyuki Wakabayashi, Yuzuru Ishimura, Masayuki Miyasaka, Tohru Takashi

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

The present study aimed to demonstrate constitutive expression of the intercellular adhesion molecule (ICAM)-1 among arterioles, capillaries, and venules in the mesentery and liver and to examine the interaction between cultured endothelial cells and leukocytes in rats. ICAM-1 expression in the microvessels in vivo was visually demonstrated by laser confocal fluorescence microscopy. A monoclonal antibody against rat ICAM-1 (1A29) was labeled with fluorescein isothiocyanate, and the binding ratio between the fluorescence and immunoglobulin was determined for data calibration. Intravascularly administered fluorescein isothiocyanate-labeled 1A29 was distributed heterogeneously among the hierarchy of microvessels in the mesentery: postcapillary venules were the major portion expressing ICAM-1 constitutively, and the density of 1A29 bound to their endothelium was at least 10 times higher than that in true capillaries and arterioles in the same mesentery. On the other hand, the liver expressed ICAM-1 abundantly in sinusoids to the extent similar to that in central venules. These results suggest that postcapillary venules serve as an active gateway with the readiness to help adhere circulating leukocytes exposed to proinflammatory stimuli in acute inflammation.

Original languageEnglish
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume273
Issue number1 42-1
Publication statusPublished - 1997 Jul

Fingerprint

Venules
Intercellular Adhesion Molecule-1
Confocal Microscopy
Mesentery
Arterioles
Microvessels
Fluorescein
Leukocytes
Liver
Fluorescence Microscopy
Calibration
Endothelium
Immunoglobulins
Cultured Cells
Endothelial Cells
Fluorescence
Monoclonal Antibodies
Inflammation
rat ICAM1 protein
isothiocyanic acid

Keywords

  • Intercellular adhesion molecule-1
  • Liver
  • Microcirculation
  • Neutrophil adhesion
  • Postcapillary venule

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Iigo, Y., Suematsu, M., Higashida, T., Oheda, J. I., Matsumoto, K., Wakabayashi, Y., ... Takashi, T. (1997). Constitutive expression of ICAM-1 in rat microvascular systems analyzed by laser confocal microscopy. American Journal of Physiology - Heart and Circulatory Physiology, 273(1 42-1).

Constitutive expression of ICAM-1 in rat microvascular systems analyzed by laser confocal microscopy. / Iigo, Yutaka; Suematsu, Makoto; Higashida, Tomoe; Oheda, Jun Ichi; Matsumoto, Keiko; Wakabayashi, Yoshiyuki; Ishimura, Yuzuru; Miyasaka, Masayuki; Takashi, Tohru.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 273, No. 1 42-1, 07.1997.

Research output: Contribution to journalArticle

Iigo, Y, Suematsu, M, Higashida, T, Oheda, JI, Matsumoto, K, Wakabayashi, Y, Ishimura, Y, Miyasaka, M & Takashi, T 1997, 'Constitutive expression of ICAM-1 in rat microvascular systems analyzed by laser confocal microscopy', American Journal of Physiology - Heart and Circulatory Physiology, vol. 273, no. 1 42-1.
Iigo, Yutaka ; Suematsu, Makoto ; Higashida, Tomoe ; Oheda, Jun Ichi ; Matsumoto, Keiko ; Wakabayashi, Yoshiyuki ; Ishimura, Yuzuru ; Miyasaka, Masayuki ; Takashi, Tohru. / Constitutive expression of ICAM-1 in rat microvascular systems analyzed by laser confocal microscopy. In: American Journal of Physiology - Heart and Circulatory Physiology. 1997 ; Vol. 273, No. 1 42-1.
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