Constitutive expression of mRNA for the same choline acetyltransferase as that in the nervous system, an acetylcholine-synthesizing enzyme, in human leukemic T-cell lines

Takeshi Fujii, Sonoko Tajima, Shin Yamada, Yoshihiro Watanabe, Kazuo Z. Sato, Marika Matsui, Hidemi Misawa, Tadashi Kasahara, Koichiro Kawashima

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Both muscarinic and nicotinic acetylcholine (ACh) receptors are known to be present on the surface of lymphocytes. We have shown that variable amounts of ACh are detectable in the blood of various mammals including humans, and a major portion of blood ACh is localized in circulating mononuclear leukocytes in humans. In order to investigate which types of blood cell are the source of ACh in human blood, expression of mRNA for choline acetyltransferase (CHAT, EC 2.3.1.6), which catalyzes ACh synthesis, was analyzed using human leukemic cell lines as models of lymphocytes and the reverse transcription- polymerase chain reaction (RT-PCR) method. We observed that mRNA for the same ChAT as that in the nervous system is expressed constitutively in all the T- cell lines tested, but not in B-, pre-lymphoma or monocytic cell lines. Furthermore, only T-cell lines showed high ACh-synthesizing activities and intracellular ACh contents. These results suggest that the major portion of ACh in the circulating blood originates from T-lymphocytes.

Original languageEnglish
Pages (from-to)71-74
Number of pages4
JournalNeuroscience Letters
Volume259
Issue number2
DOIs
Publication statusPublished - 1999 Jan 8
Externally publishedYes

Fingerprint

Choline O-Acetyltransferase
Nervous System
Acetylcholine
T-Lymphocytes
Cell Line
Messenger RNA
Enzymes
Lymphocytes
Mononuclear Leukocytes
Nicotinic Receptors
Muscarinic Receptors
Reverse Transcription
Mammals
Lymphoma
Blood Cells
Polymerase Chain Reaction

Keywords

  • Acetylcholine
  • B-Lymphocyte
  • Choline acetyltransferase
  • Radioimmunoassay
  • Reverse transcription-polymerase chain reaction
  • T- Lymphocyte

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Constitutive expression of mRNA for the same choline acetyltransferase as that in the nervous system, an acetylcholine-synthesizing enzyme, in human leukemic T-cell lines. / Fujii, Takeshi; Tajima, Sonoko; Yamada, Shin; Watanabe, Yoshihiro; Sato, Kazuo Z.; Matsui, Marika; Misawa, Hidemi; Kasahara, Tadashi; Kawashima, Koichiro.

In: Neuroscience Letters, Vol. 259, No. 2, 08.01.1999, p. 71-74.

Research output: Contribution to journalArticle

Fujii, Takeshi ; Tajima, Sonoko ; Yamada, Shin ; Watanabe, Yoshihiro ; Sato, Kazuo Z. ; Matsui, Marika ; Misawa, Hidemi ; Kasahara, Tadashi ; Kawashima, Koichiro. / Constitutive expression of mRNA for the same choline acetyltransferase as that in the nervous system, an acetylcholine-synthesizing enzyme, in human leukemic T-cell lines. In: Neuroscience Letters. 1999 ; Vol. 259, No. 2. pp. 71-74.
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