Construction and Characterization of Single-Gene Chromosomes in Saccharomyces cerevisiae

Kazuo Yamagishi, Minetaka Sugiyama, Yoshinobu Kaneko, Masafumi Nishizawa, Satoshi Harashima

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Based on a previously developed PCR-mediated chromosome splitting method, a genome engineering technique was developed in haploid Saccharomyces cerevisiae for incorporating any desired chromosomal region into a chromosome that carries a single gene. Based on the viability of cells carrying an essential gene in such a construct, close physical proximity of two telomeres and a centromere does not appear to compromise gene function. Spontaneous loss of constructed single-gene chromosomes during vegetative growth was high (0.2-0.4 per cell division), suggesting the possibility of creating novel cells carrying single-gene chromosomes derived from various chromosomal regions in a variety of combinations by exploiting combinatorial loss.

Original languageEnglish
Pages (from-to)563-567
Number of pages5
JournalJournal of Bioscience and Bioengineering
Volume106
Issue number6
DOIs
Publication statusPublished - 2008 Dec

Fingerprint

Chromosomes
Yeast
Saccharomyces cerevisiae
Genes
Centromere
Haploidy
Essential Genes
Telomere
Cells
Cell Division
Cell Survival
Genome
Polymerase Chain Reaction
Growth

Keywords

  • budding yeast
  • chromosome instability
  • chromosome loss
  • chromosome splitting
  • Cre-loxP system
  • mini-chromosome

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Construction and Characterization of Single-Gene Chromosomes in Saccharomyces cerevisiae. / Yamagishi, Kazuo; Sugiyama, Minetaka; Kaneko, Yoshinobu; Nishizawa, Masafumi; Harashima, Satoshi.

In: Journal of Bioscience and Bioengineering, Vol. 106, No. 6, 12.2008, p. 563-567.

Research output: Contribution to journalArticle

Yamagishi, Kazuo ; Sugiyama, Minetaka ; Kaneko, Yoshinobu ; Nishizawa, Masafumi ; Harashima, Satoshi. / Construction and Characterization of Single-Gene Chromosomes in Saccharomyces cerevisiae. In: Journal of Bioscience and Bioengineering. 2008 ; Vol. 106, No. 6. pp. 563-567.
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