Construction and Characterization of Single-Gene Chromosomes in Saccharomyces cerevisiae

Kazuo Yamagishi, Minetaka Sugiyama, Yoshinobu Kaneko, Masafumi Nishizawa, Satoshi Harashima

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Based on a previously developed PCR-mediated chromosome splitting method, a genome engineering technique was developed in haploid Saccharomyces cerevisiae for incorporating any desired chromosomal region into a chromosome that carries a single gene. Based on the viability of cells carrying an essential gene in such a construct, close physical proximity of two telomeres and a centromere does not appear to compromise gene function. Spontaneous loss of constructed single-gene chromosomes during vegetative growth was high (0.2-0.4 per cell division), suggesting the possibility of creating novel cells carrying single-gene chromosomes derived from various chromosomal regions in a variety of combinations by exploiting combinatorial loss.

Original languageEnglish
Pages (from-to)563-567
Number of pages5
JournalJournal of Bioscience and Bioengineering
Volume106
Issue number6
DOIs
Publication statusPublished - 2008 Dec 1

Keywords

  • Cre-loxP system
  • budding yeast
  • chromosome instability
  • chromosome loss
  • chromosome splitting
  • mini-chromosome

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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