Abstract
Background: Appropriate regulation of respective gene expressions is a bottleneck for the realization of artificial biological systems inside living cells. The modification of several promoter sequences is required to achieve appropriate regulation of the systems. However, a time-consuming process is required for the insertion of an operator, a binding site of a protein for gene expression, to the gene regulatory region of a plasmid. Thus, a standardized method for integrating operator sequences to the regulatory region of a plasmid is required.Results: We developed a standardized method for integrating operator sequences to the regulatory region of a plasmid and constructed a synthetic promoter that functions as a genetic AND gate. By standardizing the regulatory region of a plasmid and the operator parts, we established a platform for modular assembly of the operator parts. Moreover, by assembling two different operator parts on the regulatory region, we constructed a regulatory device with an AND gate function.Conclusions: We implemented a new standard to assemble operator parts for construction of functional genetic logic gates. The logic gates at the molecular scale have important implications for reprogramming cellular behavior.
| Original language | English |
|---|---|
| Article number | S16 |
| Journal | BMC Genomics |
| Volume | 11 |
| Issue number | SUPPL. 4 |
| DOIs | |
| Publication status | Published - 2010 Dec 2 |
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ASJC Scopus subject areas
- Biotechnology
- Genetics
- Agricultural and Biological Sciences (miscellaneous)
- Artificial Intelligence
- Computational Theory and Mathematics
- Computer Science Applications
- Software
Cite this
Construction of a genetic AND gate under a new standard for assembly of genetic parts. / Ayukawa, Shotaro; Kobayashi, Akio; Nakashima, Yusaku; Takagi, Hidemasa; Hamada, Shogo; Uchiyama, Masahiko; Yugi, Katsuyuki; Murata, Satoshi; Sakakibara, Yasubumi; Hagiya, Masami; Yamamura, Masayuki; Kiga, Daisuke.
In: BMC Genomics, Vol. 11, No. SUPPL. 4, S16, 02.12.2010.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Construction of a genetic AND gate under a new standard for assembly of genetic parts
AU - Ayukawa, Shotaro
AU - Kobayashi, Akio
AU - Nakashima, Yusaku
AU - Takagi, Hidemasa
AU - Hamada, Shogo
AU - Uchiyama, Masahiko
AU - Yugi, Katsuyuki
AU - Murata, Satoshi
AU - Sakakibara, Yasubumi
AU - Hagiya, Masami
AU - Yamamura, Masayuki
AU - Kiga, Daisuke
PY - 2010/12/2
Y1 - 2010/12/2
N2 - Background: Appropriate regulation of respective gene expressions is a bottleneck for the realization of artificial biological systems inside living cells. The modification of several promoter sequences is required to achieve appropriate regulation of the systems. However, a time-consuming process is required for the insertion of an operator, a binding site of a protein for gene expression, to the gene regulatory region of a plasmid. Thus, a standardized method for integrating operator sequences to the regulatory region of a plasmid is required.Results: We developed a standardized method for integrating operator sequences to the regulatory region of a plasmid and constructed a synthetic promoter that functions as a genetic AND gate. By standardizing the regulatory region of a plasmid and the operator parts, we established a platform for modular assembly of the operator parts. Moreover, by assembling two different operator parts on the regulatory region, we constructed a regulatory device with an AND gate function.Conclusions: We implemented a new standard to assemble operator parts for construction of functional genetic logic gates. The logic gates at the molecular scale have important implications for reprogramming cellular behavior.
AB - Background: Appropriate regulation of respective gene expressions is a bottleneck for the realization of artificial biological systems inside living cells. The modification of several promoter sequences is required to achieve appropriate regulation of the systems. However, a time-consuming process is required for the insertion of an operator, a binding site of a protein for gene expression, to the gene regulatory region of a plasmid. Thus, a standardized method for integrating operator sequences to the regulatory region of a plasmid is required.Results: We developed a standardized method for integrating operator sequences to the regulatory region of a plasmid and constructed a synthetic promoter that functions as a genetic AND gate. By standardizing the regulatory region of a plasmid and the operator parts, we established a platform for modular assembly of the operator parts. Moreover, by assembling two different operator parts on the regulatory region, we constructed a regulatory device with an AND gate function.Conclusions: We implemented a new standard to assemble operator parts for construction of functional genetic logic gates. The logic gates at the molecular scale have important implications for reprogramming cellular behavior.
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UR - http://www.scopus.com/inward/citedby.url?scp=78649774637&partnerID=8YFLogxK
U2 - 10.1186/1471-2164-11-S4-S16
DO - 10.1186/1471-2164-11-S4-S16
M3 - Article
C2 - 21143799
AN - SCOPUS:78649774637
VL - 11
JO - BMC Genomics
JF - BMC Genomics
SN - 1471-2164
IS - SUPPL. 4
M1 - S16
ER -