Construction of a metagenomic library for the marine sponge Halichondria okadai

Takahiro Abe, Fatma Pinar Sahin, Kiyotaka Akiyama, Takayuki Naito, Mizoe Kishigami, Kenji Miyamoto, Yasufumi Sakakibara, Daisuke Uemura

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Symbionts of the marine sponge Halichondria okadai are promising as a source of natural products. Metagenomic technology is a powerful tool for accessing the genetic and biochemical potential of bacteria. Hence, we established a method of recovering bacterial-enriched metagenomic DNA by stepwise centrifugation. The metagenomic DNA was analyzed by ultrafast 454- pyrosequencing technology, and the results suggested that more than three types of bacterial DNA, Alphaproteobacteria, Actinobacteria, and Cyanobacteria, had been recovered, and that eukaryotic genes comprised only 0.02% of the metagenomic DNA. These results indicate that stepwise centrifugation and real-time quantitative PCR were effective for separating sponge cells and symbiotic bacteria, and that we constructed a bacteria-enriched metagenomic library from a marine sponge, H. okadai, selectively for the first time.

Original languageEnglish
Pages (from-to)633-639
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume76
Issue number4
DOIs
Publication statusPublished - 2012 Apr 30

Keywords

  • 454-pyrosequencing
  • Fosmid
  • Halichondria okadai
  • Metagenomic library
  • Sponge

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Construction of a metagenomic library for the marine sponge Halichondria okadai'. Together they form a unique fingerprint.

  • Cite this

    Abe, T., Sahin, F. P., Akiyama, K., Naito, T., Kishigami, M., Miyamoto, K., Sakakibara, Y., & Uemura, D. (2012). Construction of a metagenomic library for the marine sponge Halichondria okadai. Bioscience, Biotechnology and Biochemistry, 76(4), 633-639. https://doi.org/10.1271/bbb.110533