Construction of a metagenomic library for the marine sponge Halichondria okadai

Takahiro Abe, Fatma Pinar Sahin, Kiyotaka Akiyama, Takayuki Naito, Mizoe Kishigami, Kenji Miyamoto, Yasufumi Sakakibara, Daisuke Uemura

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Symbionts of the marine sponge Halichondria okadai are promising as a source of natural products. Metagenomic technology is a powerful tool for accessing the genetic and biochemical potential of bacteria. Hence, we established a method of recovering bacterial-enriched metagenomic DNA by stepwise centrifugation. The metagenomic DNA was analyzed by ultrafast 454- pyrosequencing technology, and the results suggested that more than three types of bacterial DNA, Alphaproteobacteria, Actinobacteria, and Cyanobacteria, had been recovered, and that eukaryotic genes comprised only 0.02% of the metagenomic DNA. These results indicate that stepwise centrifugation and real-time quantitative PCR were effective for separating sponge cells and symbiotic bacteria, and that we constructed a bacteria-enriched metagenomic library from a marine sponge, H. okadai, selectively for the first time.

Original languageEnglish
Pages (from-to)633-639
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume76
Issue number4
DOIs
Publication statusPublished - 2012

Fingerprint

Metagenomics
Porifera
Bacteria
Centrifugation
DNA
Bacterial DNA
Biological Products
Technology
Alphaproteobacteria
Genes
Actinobacteria
Cyanobacteria
Real-Time Polymerase Chain Reaction
Molecular Biology

Keywords

  • 454-pyrosequencing
  • Fosmid
  • Halichondria okadai
  • Metagenomic library
  • Sponge

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Construction of a metagenomic library for the marine sponge Halichondria okadai. / Abe, Takahiro; Sahin, Fatma Pinar; Akiyama, Kiyotaka; Naito, Takayuki; Kishigami, Mizoe; Miyamoto, Kenji; Sakakibara, Yasufumi; Uemura, Daisuke.

In: Bioscience, Biotechnology and Biochemistry, Vol. 76, No. 4, 2012, p. 633-639.

Research output: Contribution to journalArticle

Abe, T, Sahin, FP, Akiyama, K, Naito, T, Kishigami, M, Miyamoto, K, Sakakibara, Y & Uemura, D 2012, 'Construction of a metagenomic library for the marine sponge Halichondria okadai', Bioscience, Biotechnology and Biochemistry, vol. 76, no. 4, pp. 633-639. https://doi.org/10.1271/bbb.110533
Abe, Takahiro ; Sahin, Fatma Pinar ; Akiyama, Kiyotaka ; Naito, Takayuki ; Kishigami, Mizoe ; Miyamoto, Kenji ; Sakakibara, Yasufumi ; Uemura, Daisuke. / Construction of a metagenomic library for the marine sponge Halichondria okadai. In: Bioscience, Biotechnology and Biochemistry. 2012 ; Vol. 76, No. 4. pp. 633-639.
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