Construction of in vitro cardiomyocyte electrical conduction line

Mariko Kurotsu; Naoki Machida; Emiyu Ogawa; Tsunenori Arai

doi:10.11239/jsmbe.52.O-123

Construction of in vitro cardiomyocyte electrical conduction line

Mariko Kurotsu, Naoki Machida, Emiyu Ogawa, Tsunenori Arai

Department of Applied Physics and Physico-Informatics

Research output: Contribution to journal › Article › peer-review

Abstract

In order to study condition of arrhythmia treatment by a photosensitization reaction, we developed in vitro cardiomyocyte electrical conduction (EC) line to study EC block by photosensitization reaction. Cardiomyocytes were cultivated on 10 mmφ cover glasses. Cultivated and non-cultivated width were 60 and 300 μm, respectively; for 3-4 days, 37C°, 5% CO<inf>2</inf>. Cardiomyocyte cultivate concentration were 2.0-8.0×105 cells/ml. EC of cardiomyocite was measured by 5 μM Fluo-4 AM (Ca2+ probe) with a confocal microscope system. Frame rate of EM-CCD was 400 ms. Cadiomyocyte was cultivated in a confluent line by 4.0×105 cells/ml. In 4 days cultivated 4.0×105 cells/ml cardiomyocite line, 1 /s synchronized pulsation was measured as Fluo-4 AM intensity change. Cross correlation coefficient was about 0.8. This result indicated that cardiomyocyte EC was occurred.

Original language	English
Pages (from-to)	O-123-O-124
Journal	Transactions of Japanese Society for Medical and Biological Engineering
Volume	52
DOIs	https://doi.org/10.11239/jsmbe.52.O-123
Publication status	Published - 2014 Aug 17

Keywords

Electrophysiology
Myocardium
Photodynamic therapy

ASJC Scopus subject areas

Biomedical Engineering

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10.11239/jsmbe.52.O-123

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title = "Construction of in vitro cardiomyocyte electrical conduction line",

abstract = "In order to study condition of arrhythmia treatment by a photosensitization reaction, we developed in vitro cardiomyocyte electrical conduction (EC) line to study EC block by photosensitization reaction. Cardiomyocytes were cultivated on 10 mmφ cover glasses. Cultivated and non-cultivated width were 60 and 300 μm, respectively; for 3-4 days, 37C°, 5% CO2. Cardiomyocyte cultivate concentration were 2.0-8.0×105 cells/ml. EC of cardiomyocite was measured by 5 μM Fluo-4 AM (Ca2+ probe) with a confocal microscope system. Frame rate of EM-CCD was 400 ms. Cadiomyocyte was cultivated in a confluent line by 4.0×105 cells/ml. In 4 days cultivated 4.0×105 cells/ml cardiomyocite line, 1 /s synchronized pulsation was measured as Fluo-4 AM intensity change. Cross correlation coefficient was about 0.8. This result indicated that cardiomyocyte EC was occurred.",

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AU - Kurotsu, Mariko

AU - Machida, Naoki

AU - Ogawa, Emiyu

AU - Arai, Tsunenori

PY - 2014/8/17

Y1 - 2014/8/17

N2 - In order to study condition of arrhythmia treatment by a photosensitization reaction, we developed in vitro cardiomyocyte electrical conduction (EC) line to study EC block by photosensitization reaction. Cardiomyocytes were cultivated on 10 mmφ cover glasses. Cultivated and non-cultivated width were 60 and 300 μm, respectively; for 3-4 days, 37C°, 5% CO2. Cardiomyocyte cultivate concentration were 2.0-8.0×105 cells/ml. EC of cardiomyocite was measured by 5 μM Fluo-4 AM (Ca2+ probe) with a confocal microscope system. Frame rate of EM-CCD was 400 ms. Cadiomyocyte was cultivated in a confluent line by 4.0×105 cells/ml. In 4 days cultivated 4.0×105 cells/ml cardiomyocite line, 1 /s synchronized pulsation was measured as Fluo-4 AM intensity change. Cross correlation coefficient was about 0.8. This result indicated that cardiomyocyte EC was occurred.

AB - In order to study condition of arrhythmia treatment by a photosensitization reaction, we developed in vitro cardiomyocyte electrical conduction (EC) line to study EC block by photosensitization reaction. Cardiomyocytes were cultivated on 10 mmφ cover glasses. Cultivated and non-cultivated width were 60 and 300 μm, respectively; for 3-4 days, 37C°, 5% CO2. Cardiomyocyte cultivate concentration were 2.0-8.0×105 cells/ml. EC of cardiomyocite was measured by 5 μM Fluo-4 AM (Ca2+ probe) with a confocal microscope system. Frame rate of EM-CCD was 400 ms. Cadiomyocyte was cultivated in a confluent line by 4.0×105 cells/ml. In 4 days cultivated 4.0×105 cells/ml cardiomyocite line, 1 /s synchronized pulsation was measured as Fluo-4 AM intensity change. Cross correlation coefficient was about 0.8. This result indicated that cardiomyocyte EC was occurred.

KW - Electrophysiology

KW - Myocardium

KW - Photodynamic therapy

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Construction of in vitro cardiomyocyte electrical conduction line

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Keywords

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