Construction of in vitro cardiomyocyte electrical conduction line

Mariko Kurotsu, Naoki Machida, Emiyu Ogawa, Tsunenori Arai

Research output: Contribution to journalArticle

Abstract

In order to study condition of arrhythmia treatment by a photosensitization reaction, we developed in vitro cardiomyocyte electrical conduction (EC) line to study EC block by photosensitization reaction. Cardiomyocytes were cultivated on 10 mmφ cover glasses. Cultivated and non-cultivated width were 60 and 300 μm, respectively; for 3-4 days, 37C°, 5% CO<inf>2</inf>. Cardiomyocyte cultivate concentration were 2.0-8.0×10<sup>5</sup> cells/ml. EC of cardiomyocite was measured by 5 μM Fluo-4 AM (Ca<sup>2+</sup> probe) with a confocal microscope system. Frame rate of EM-CCD was 400 ms. Cadiomyocyte was cultivated in a confluent line by 4.0×10<sup>5</sup> cells/ml. In 4 days cultivated 4.0×10<sup>5</sup> cells/ml cardiomyocite line, 1 /s synchronized pulsation was measured as Fluo-4 AM intensity change. Cross correlation coefficient was about 0.8. This result indicated that cardiomyocyte EC was occurred.

Original languageEnglish
Pages (from-to)O-123-O-124
JournalTransactions of Japanese Society for Medical and Biological Engineering
Volume52
DOIs
Publication statusPublished - 2014 Aug 17

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Keywords

  • Electrophysiology
  • Myocardium
  • Photodynamic therapy

ASJC Scopus subject areas

  • Biomedical Engineering

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