Construction of the Bacillus subtilis chromosome physical map and the strategy for mapping newly isolated genes in one membrane filter for hybridization.

Mitsuhiro Itaya

Research output: Contribution to journalArticle

Abstract

A complete physical map of the Bacillus subtilis 168 chromosome was constructed. The merging of this physical map is expected not only to provide important insights into the organization and rearrangement of genes of this species but also to be a powerful means for the genome analysis. One of the most practical aspects is rapid and accurate mapping of newly isolated genes using a single membrane filter for hybridization. This protocol proved that not only unique genes but also multiple homologous genes dispersed on the chromosome can be physically mapped.

Original languageEnglish
Pages (from-to)145-146
Number of pages2
JournalNucleic acids symposium series
Issue number29
Publication statusPublished - 1993
Externally publishedYes

Fingerprint

Bacillus subtilis
Chromosomes
Membranes
Genes
Gene Rearrangement
Genome

ASJC Scopus subject areas

  • Medicine(all)

Cite this

@article{01a4491ae1724ed5a2f22dbcacf299b4,
title = "Construction of the Bacillus subtilis chromosome physical map and the strategy for mapping newly isolated genes in one membrane filter for hybridization.",
abstract = "A complete physical map of the Bacillus subtilis 168 chromosome was constructed. The merging of this physical map is expected not only to provide important insights into the organization and rearrangement of genes of this species but also to be a powerful means for the genome analysis. One of the most practical aspects is rapid and accurate mapping of newly isolated genes using a single membrane filter for hybridization. This protocol proved that not only unique genes but also multiple homologous genes dispersed on the chromosome can be physically mapped.",
author = "Mitsuhiro Itaya",
year = "1993",
language = "English",
pages = "145--146",
journal = "Nucleic acids symposium series",
issn = "0261-3166",
publisher = "Information Retrieval",
number = "29",

}

TY - JOUR

T1 - Construction of the Bacillus subtilis chromosome physical map and the strategy for mapping newly isolated genes in one membrane filter for hybridization.

AU - Itaya, Mitsuhiro

PY - 1993

Y1 - 1993

N2 - A complete physical map of the Bacillus subtilis 168 chromosome was constructed. The merging of this physical map is expected not only to provide important insights into the organization and rearrangement of genes of this species but also to be a powerful means for the genome analysis. One of the most practical aspects is rapid and accurate mapping of newly isolated genes using a single membrane filter for hybridization. This protocol proved that not only unique genes but also multiple homologous genes dispersed on the chromosome can be physically mapped.

AB - A complete physical map of the Bacillus subtilis 168 chromosome was constructed. The merging of this physical map is expected not only to provide important insights into the organization and rearrangement of genes of this species but also to be a powerful means for the genome analysis. One of the most practical aspects is rapid and accurate mapping of newly isolated genes using a single membrane filter for hybridization. This protocol proved that not only unique genes but also multiple homologous genes dispersed on the chromosome can be physically mapped.

UR - http://www.scopus.com/inward/record.url?scp=0027480704&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027480704&partnerID=8YFLogxK

M3 - Article

C2 - 8247744

AN - SCOPUS:0027480704

SP - 145

EP - 146

JO - Nucleic acids symposium series

JF - Nucleic acids symposium series

SN - 0261-3166

IS - 29

ER -