Construction of the Bacillus subtilis chromosome physical map and the strategy for mapping newly isolated genes in one membrane filter for hybridization.

M. Itaya

Research output: Contribution to journalArticle

Abstract

A complete physical map of the Bacillus subtilis 168 chromosome was constructed. The merging of this physical map is expected not only to provide important insights into the organization and rearrangement of genes of this species but also to be a powerful means for the genome analysis. One of the most practical aspects is rapid and accurate mapping of newly isolated genes using a single membrane filter for hybridization. This protocol proved that not only unique genes but also multiple homologous genes dispersed on the chromosome can be physically mapped.

Original languageEnglish
Pages (from-to)145-146
Number of pages2
JournalNucleic acids symposium series
Issue number29
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Medicine(all)

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