Construction of yeast secretion vectors designed for production of mature proteins using the signal sequence of yeast invertase

Masafumi Nishizawa, Fumiko Ozawa, Fumio Hishinuma

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2 Citations (Scopus)

Abstract

Two kinds of yeast secretion vectors were constructed by site-directed mutagenesis of the invertase signal sequence and ligation of synthetic oligonucleotides coding appropriate signals. Each has a cloning site for a foreign gene preceded by a sequence encoding either the signal peptide cleavage site or a Lys-Arg sequence which is a cleavage site for the product of the KEX2 gene. Both vectors were able to direct the expression and secretion of mouse amylase. One of them has a SalI site within the signal sequence, and an attempt to clone sequences enhancing secretion of amylase with this vector is reported.

Original languageEnglish
Pages (from-to)317-322
Number of pages6
JournalApplied Microbiology and Biotechnology
Volume32
Issue number3
DOIs
Publication statusPublished - 1989 Dec 1

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ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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