Continuous and selective measurement of oxytocin and vasopressin using boron-doped diamond electrodes

Kai Asai, Tribidasari A. Ivandini, Yasuaki Einaga

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The electrochemical detection of oxytocin using boron-doped diamond (BDD) electrodes was studied. Cyclic voltammetry of oxytocin in a phosphate buffer solution exhibits an oxidation peak at +0.7 V (vs. Ag/AgCl), which is attributable to oxidation of the phenolic group in the tyrosyl moiety. Furthermore, the linearity of the current peaks obtained in flow injection analysis (FIA) using BDD microelectrodes over the oxytocin concentration range from 0.1 to 10.0 μM with a detection limit of 50 nM (S/N = 3) was high (R2 = 0.995). Although the voltammograms of oxytocin and vasopressin observed with an as-deposited BDD electrode, as well as with a cathodically-reduced BDD electrode, were similar, a clear distinction was observed with anodically-oxidized BDD electrodes due to the attractive interaction between vasopressin and the oxidized BDD surface. By means of this distinction, selective measurements using chronoamperometry combined with flow injection analysis at an optimized potential were demonstrated, indicating the possibility of making selective in situ or in vivo measurements of oxytocin.

Original languageEnglish
Article number32429
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 2016 Sep 7

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Diamond
Boron
Oxytocin
Vasopressins
Electrodes
Flow Injection Analysis
Microelectrodes
Limit of Detection
Buffers
Phosphates

ASJC Scopus subject areas

  • General

Cite this

Continuous and selective measurement of oxytocin and vasopressin using boron-doped diamond electrodes. / Asai, Kai; Ivandini, Tribidasari A.; Einaga, Yasuaki.

In: Scientific Reports, Vol. 6, 32429, 07.09.2016.

Research output: Contribution to journalArticle

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