Continuous depolarization induces choline acetyltransferase activity in septal and hippocampal co-cultured embryonic rat neurons

Takeshi Suzuki, Kumiko Shimotohno

Research output: Contribution to journalArticle


We tested the influence of continuous high-K+ treatment on acetylcholine (ACh) release and choline acetyltransferase (ChAT) activity on septal cell culture, and septal and hippocampal cell co-culture obtained from rat embryos. Continuous 9 mM K+ treatment did not affect ACh release and ChAT activity in septal culture, but increased ACh release in co-culture without affecting ChAT activity. A slight increase in extracellular K + concentration, therefore, induced neuronal excitation. Continuous 55 mM K+ treatment increased ACh release in septal culture. This effect was due to direct excitation of septal neurons. In co-culture, 55 mM K+ treatment increased both ACh release and ChAT activity. These results indicate that hippocampal neurons are indispensable for the depolarization-induced increase in ChAT activity in the early stage of developing septal cholinergic neurons.

Original languageEnglish
Pages (from-to)79-81
Number of pages3
JournalNeuroscience Letters
Issue number1
Publication statusPublished - 2006 Feb 6



  • Acetylcholine
  • Choline acetyltransferase
  • Culture
  • Depolarization
  • Hippocampus
  • Septum

ASJC Scopus subject areas

  • Neuroscience(all)

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