Continuous optical measurement system of hemolysis during a photosensitization reaction using absorption spectrum

R. Hamada, E. Ogawa, Tsunenori Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

To investigate hemolysis phenomena during a photosensitization reaction with the reaction condition continuously and simultaneously for a safety assessment of hemolysis side effect, we constructed an optical system to measure blood sample absorption spectrum during the reaction. Hemolysis degree might be under estimated in general evaluation methods because there is a constant oxygen pressure assumption in spite of oxygen depression take place. By investigating hemoglobin oxidation and oxygen desorption dynamics obtained from the contribution of the visible absorption spectrum and multiple regression analysis, both the hemolysis phenomena and its oxygen environment might be obtained with time. A 664 nm wavelength laser beam for the reaction excitation and 475-650 nm light beam for measuring the absorbance spectrum were arranged perpendicularly crossing. A quartz glass cuvette with 1×10 mm in dimensions for the spectrum measurement was located at this crossing point. A red blood cells suspension medium was arranged with low hematocrit containing 30 μg/ml talaporfin sodium. This medium was irradiated up to 40 J/cm2. The met-hemoglobin, oxygenatedhemoglobin, and deoxygenated-hemoglobin concentrations were calculated by a multiple regression analysis from the measured spectra. We confirmed the met-hemoglobin concentration increased and oxygen saturation decreased with the irradiation time, which seems to indicate the hemolysis progression and oxygen consumption, respectively. By using our measuring system, the hemolysis progression seems to be obtained with oxygen environment information.

Original languageEnglish
Title of host publicationOptical Interactions with Tissue and Cells XXIX
PublisherSPIE
Volume10492
ISBN (Electronic)9781510614697
DOIs
Publication statusPublished - 2018 Jan 1
EventOptical Interactions with Tissue and Cells XXIX 2018 - San Francisco, United States
Duration: 2018 Jan 292018 Jan 30

Other

OtherOptical Interactions with Tissue and Cells XXIX 2018
CountryUnited States
CitySan Francisco
Period18/1/2918/1/30

Fingerprint

hemolysis
Photosensitivity Disorders
Optical Devices
Hemolysis
optical measurement
Absorption spectra
hemoglobin
Oxygen
absorption spectra
Hemoglobin
oxygen
Methemoglobin
progressions
regression analysis
Regression analysis
Hemoglobins
Regression Analysis
hematocrit
Blood
oxygen consumption

Keywords

  • Absorption spectrum
  • Hemoglobin
  • Hemolysis
  • Multiple regression analysis
  • Photosensitization reaction

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Hamada, R., Ogawa, E., & Arai, T. (2018). Continuous optical measurement system of hemolysis during a photosensitization reaction using absorption spectrum. In Optical Interactions with Tissue and Cells XXIX (Vol. 10492). [104920J] SPIE. https://doi.org/10.1117/12.2287055

Continuous optical measurement system of hemolysis during a photosensitization reaction using absorption spectrum. / Hamada, R.; Ogawa, E.; Arai, Tsunenori.

Optical Interactions with Tissue and Cells XXIX. Vol. 10492 SPIE, 2018. 104920J.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hamada, R, Ogawa, E & Arai, T 2018, Continuous optical measurement system of hemolysis during a photosensitization reaction using absorption spectrum. in Optical Interactions with Tissue and Cells XXIX. vol. 10492, 104920J, SPIE, Optical Interactions with Tissue and Cells XXIX 2018, San Francisco, United States, 18/1/29. https://doi.org/10.1117/12.2287055
Hamada R, Ogawa E, Arai T. Continuous optical measurement system of hemolysis during a photosensitization reaction using absorption spectrum. In Optical Interactions with Tissue and Cells XXIX. Vol. 10492. SPIE. 2018. 104920J https://doi.org/10.1117/12.2287055
Hamada, R. ; Ogawa, E. ; Arai, Tsunenori. / Continuous optical measurement system of hemolysis during a photosensitization reaction using absorption spectrum. Optical Interactions with Tissue and Cells XXIX. Vol. 10492 SPIE, 2018.
@inproceedings{f3841e55d386462b9fd434f592158e6e,
title = "Continuous optical measurement system of hemolysis during a photosensitization reaction using absorption spectrum",
abstract = "To investigate hemolysis phenomena during a photosensitization reaction with the reaction condition continuously and simultaneously for a safety assessment of hemolysis side effect, we constructed an optical system to measure blood sample absorption spectrum during the reaction. Hemolysis degree might be under estimated in general evaluation methods because there is a constant oxygen pressure assumption in spite of oxygen depression take place. By investigating hemoglobin oxidation and oxygen desorption dynamics obtained from the contribution of the visible absorption spectrum and multiple regression analysis, both the hemolysis phenomena and its oxygen environment might be obtained with time. A 664 nm wavelength laser beam for the reaction excitation and 475-650 nm light beam for measuring the absorbance spectrum were arranged perpendicularly crossing. A quartz glass cuvette with 1×10 mm in dimensions for the spectrum measurement was located at this crossing point. A red blood cells suspension medium was arranged with low hematocrit containing 30 μg/ml talaporfin sodium. This medium was irradiated up to 40 J/cm2. The met-hemoglobin, oxygenatedhemoglobin, and deoxygenated-hemoglobin concentrations were calculated by a multiple regression analysis from the measured spectra. We confirmed the met-hemoglobin concentration increased and oxygen saturation decreased with the irradiation time, which seems to indicate the hemolysis progression and oxygen consumption, respectively. By using our measuring system, the hemolysis progression seems to be obtained with oxygen environment information.",
keywords = "Absorption spectrum, Hemoglobin, Hemolysis, Multiple regression analysis, Photosensitization reaction",
author = "R. Hamada and E. Ogawa and Tsunenori Arai",
year = "2018",
month = "1",
day = "1",
doi = "10.1117/12.2287055",
language = "English",
volume = "10492",
booktitle = "Optical Interactions with Tissue and Cells XXIX",
publisher = "SPIE",

}

TY - GEN

T1 - Continuous optical measurement system of hemolysis during a photosensitization reaction using absorption spectrum

AU - Hamada, R.

AU - Ogawa, E.

AU - Arai, Tsunenori

PY - 2018/1/1

Y1 - 2018/1/1

N2 - To investigate hemolysis phenomena during a photosensitization reaction with the reaction condition continuously and simultaneously for a safety assessment of hemolysis side effect, we constructed an optical system to measure blood sample absorption spectrum during the reaction. Hemolysis degree might be under estimated in general evaluation methods because there is a constant oxygen pressure assumption in spite of oxygen depression take place. By investigating hemoglobin oxidation and oxygen desorption dynamics obtained from the contribution of the visible absorption spectrum and multiple regression analysis, both the hemolysis phenomena and its oxygen environment might be obtained with time. A 664 nm wavelength laser beam for the reaction excitation and 475-650 nm light beam for measuring the absorbance spectrum were arranged perpendicularly crossing. A quartz glass cuvette with 1×10 mm in dimensions for the spectrum measurement was located at this crossing point. A red blood cells suspension medium was arranged with low hematocrit containing 30 μg/ml talaporfin sodium. This medium was irradiated up to 40 J/cm2. The met-hemoglobin, oxygenatedhemoglobin, and deoxygenated-hemoglobin concentrations were calculated by a multiple regression analysis from the measured spectra. We confirmed the met-hemoglobin concentration increased and oxygen saturation decreased with the irradiation time, which seems to indicate the hemolysis progression and oxygen consumption, respectively. By using our measuring system, the hemolysis progression seems to be obtained with oxygen environment information.

AB - To investigate hemolysis phenomena during a photosensitization reaction with the reaction condition continuously and simultaneously for a safety assessment of hemolysis side effect, we constructed an optical system to measure blood sample absorption spectrum during the reaction. Hemolysis degree might be under estimated in general evaluation methods because there is a constant oxygen pressure assumption in spite of oxygen depression take place. By investigating hemoglobin oxidation and oxygen desorption dynamics obtained from the contribution of the visible absorption spectrum and multiple regression analysis, both the hemolysis phenomena and its oxygen environment might be obtained with time. A 664 nm wavelength laser beam for the reaction excitation and 475-650 nm light beam for measuring the absorbance spectrum were arranged perpendicularly crossing. A quartz glass cuvette with 1×10 mm in dimensions for the spectrum measurement was located at this crossing point. A red blood cells suspension medium was arranged with low hematocrit containing 30 μg/ml talaporfin sodium. This medium was irradiated up to 40 J/cm2. The met-hemoglobin, oxygenatedhemoglobin, and deoxygenated-hemoglobin concentrations were calculated by a multiple regression analysis from the measured spectra. We confirmed the met-hemoglobin concentration increased and oxygen saturation decreased with the irradiation time, which seems to indicate the hemolysis progression and oxygen consumption, respectively. By using our measuring system, the hemolysis progression seems to be obtained with oxygen environment information.

KW - Absorption spectrum

KW - Hemoglobin

KW - Hemolysis

KW - Multiple regression analysis

KW - Photosensitization reaction

UR - http://www.scopus.com/inward/record.url?scp=85047375844&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047375844&partnerID=8YFLogxK

U2 - 10.1117/12.2287055

DO - 10.1117/12.2287055

M3 - Conference contribution

AN - SCOPUS:85047375844

VL - 10492

BT - Optical Interactions with Tissue and Cells XXIX

PB - SPIE

ER -