Continuous optical measurement system of hemolysis during a photosensitization reaction using absorption spectrum

R. Hamada, E. Ogawa, T. Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)


To investigate hemolysis phenomena during a photosensitization reaction with the reaction condition continuously and simultaneously for a safety assessment of hemolysis side effect, we constructed an optical system to measure blood sample absorption spectrum during the reaction. Hemolysis degree might be under estimated in general evaluation methods because there is a constant oxygen pressure assumption in spite of oxygen depression take place. By investigating hemoglobin oxidation and oxygen desorption dynamics obtained from the contribution of the visible absorption spectrum and multiple regression analysis, both the hemolysis phenomena and its oxygen environment might be obtained with time. A 664 nm wavelength laser beam for the reaction excitation and 475-650 nm light beam for measuring the absorbance spectrum were arranged perpendicularly crossing. A quartz glass cuvette with 1×10 mm in dimensions for the spectrum measurement was located at this crossing point. A red blood cells suspension medium was arranged with low hematocrit containing 30 μg/ml talaporfin sodium. This medium was irradiated up to 40 J/cm2. The met-hemoglobin, oxygenatedhemoglobin, and deoxygenated-hemoglobin concentrations were calculated by a multiple regression analysis from the measured spectra. We confirmed the met-hemoglobin concentration increased and oxygen saturation decreased with the irradiation time, which seems to indicate the hemolysis progression and oxygen consumption, respectively. By using our measuring system, the hemolysis progression seems to be obtained with oxygen environment information.

Original languageEnglish
Title of host publicationOptical Interactions with Tissue and Cells XXIX
EditorsE. Duco Jansen, Hope Thomas Beier
ISBN (Electronic)9781510614697
Publication statusPublished - 2018
EventOptical Interactions with Tissue and Cells XXIX 2018 - San Francisco, United States
Duration: 2018 Jan 292018 Jan 30

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherOptical Interactions with Tissue and Cells XXIX 2018
Country/TerritoryUnited States
CitySan Francisco


  • Absorption spectrum
  • Hemoglobin
  • Hemolysis
  • Multiple regression analysis
  • Photosensitization reaction

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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