Continuous optical monitoring of red blood cells during a photosensitization reaction

Risa Hamada, Emiyu Ogawa, Tsunenori Arai

Research output: Contribution to journalArticle

Abstract

Background: An oxygen-enriched photosensitizer solution was created by the addition of red blood cells (RBCs) as an investigative tool for photosensitization reactions (PRs). Although the oxygen levels and reaction progress can be monitored using the optical characteristics of hemoglobin, previously this has only been done using intermittent measurements. An increase in methemoglobin concentration with irradiation time was reported. Objective: We constructed a continuous optical measurement system to study the dynamics of the PR in a photosensitizer solution containing RBCs. We also measured the relationship between hemolysis and methemoglobin production in the solution. Materials and methods: A 664 nm wavelength continuous laser beam at 60 mW/cm 2 was used to drive the PR, and a broadband (475-650 nm) light beam was used to monitor the absorption spectra during the PR. The light sources were arranged perpendicularly to cross at a 1 × 10 mm cuvette. The sample in this cuvette was prepared from a low-hematocrit rabbit RBC suspension medium containing 30 μg/mL talaporfin sodium, a chlorine photosensitizer. The concentrations of oxygenated hemoglobin, deoxygenated hemoglobin, and methemoglobin were obtained using a multiple regression analysis of the measured spectra. Results: The oxygen saturation decreased continuously during the PR. The relationship between the degree of hemolysis and produced methemoglobin concentration was confirmed. Conclusions: We determined the dynamics of the oxidation and oxygen desorption of hemoglobin, as well as RBC hemolysis, during the PR. Our measurement system, which uses the properties of hemoglobin contained in RBCs, might be useful for continuous monitoring of PR dynamics.

Original languageEnglish
Pages (from-to)110-116
Number of pages7
JournalPhotobiomodulation, Photomedicine, and Laser Surgery
Volume37
Issue number2
DOIs
Publication statusPublished - 2019 Feb 1

Fingerprint

Photosensitivity Disorders
Hemoglobin
Blood
Erythrocytes
Cells
Methemoglobin
Photosensitizers
Hemoglobins
Monitoring
Photosensitizing Agents
Oxygen
Hemolysis
Regression analysis
Light
Chlorine
Optical Devices
Laser beams
Light sources
Absorption spectra
Desorption

Keywords

  • absorption spectrum
  • hemoglobin
  • hemolysis
  • multiple regression analysis
  • oxygen level
  • photosensitization reaction

ASJC Scopus subject areas

  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

Cite this

Continuous optical monitoring of red blood cells during a photosensitization reaction. / Hamada, Risa; Ogawa, Emiyu; Arai, Tsunenori.

In: Photobiomodulation, Photomedicine, and Laser Surgery, Vol. 37, No. 2, 01.02.2019, p. 110-116.

Research output: Contribution to journalArticle

Hamada, Risa ; Ogawa, Emiyu ; Arai, Tsunenori. / Continuous optical monitoring of red blood cells during a photosensitization reaction. In: Photobiomodulation, Photomedicine, and Laser Surgery. 2019 ; Vol. 37, No. 2. pp. 110-116.
@article{72ea262ad3e9443c81294dae8c984853,
title = "Continuous optical monitoring of red blood cells during a photosensitization reaction",
abstract = "Background: An oxygen-enriched photosensitizer solution was created by the addition of red blood cells (RBCs) as an investigative tool for photosensitization reactions (PRs). Although the oxygen levels and reaction progress can be monitored using the optical characteristics of hemoglobin, previously this has only been done using intermittent measurements. An increase in methemoglobin concentration with irradiation time was reported. Objective: We constructed a continuous optical measurement system to study the dynamics of the PR in a photosensitizer solution containing RBCs. We also measured the relationship between hemolysis and methemoglobin production in the solution. Materials and methods: A 664 nm wavelength continuous laser beam at 60 mW/cm 2 was used to drive the PR, and a broadband (475-650 nm) light beam was used to monitor the absorption spectra during the PR. The light sources were arranged perpendicularly to cross at a 1 × 10 mm cuvette. The sample in this cuvette was prepared from a low-hematocrit rabbit RBC suspension medium containing 30 μg/mL talaporfin sodium, a chlorine photosensitizer. The concentrations of oxygenated hemoglobin, deoxygenated hemoglobin, and methemoglobin were obtained using a multiple regression analysis of the measured spectra. Results: The oxygen saturation decreased continuously during the PR. The relationship between the degree of hemolysis and produced methemoglobin concentration was confirmed. Conclusions: We determined the dynamics of the oxidation and oxygen desorption of hemoglobin, as well as RBC hemolysis, during the PR. Our measurement system, which uses the properties of hemoglobin contained in RBCs, might be useful for continuous monitoring of PR dynamics.",
keywords = "absorption spectrum, hemoglobin, hemolysis, multiple regression analysis, oxygen level, photosensitization reaction",
author = "Risa Hamada and Emiyu Ogawa and Tsunenori Arai",
year = "2019",
month = "2",
day = "1",
doi = "10.1089/photob.2018.4513",
language = "English",
volume = "37",
pages = "110--116",
journal = "Photobiomodulation, Photomedicine, and Laser Surgery",
issn = "2578-5478",
publisher = "Mary Ann Liebert Inc.",
number = "2",

}

TY - JOUR

T1 - Continuous optical monitoring of red blood cells during a photosensitization reaction

AU - Hamada, Risa

AU - Ogawa, Emiyu

AU - Arai, Tsunenori

PY - 2019/2/1

Y1 - 2019/2/1

N2 - Background: An oxygen-enriched photosensitizer solution was created by the addition of red blood cells (RBCs) as an investigative tool for photosensitization reactions (PRs). Although the oxygen levels and reaction progress can be monitored using the optical characteristics of hemoglobin, previously this has only been done using intermittent measurements. An increase in methemoglobin concentration with irradiation time was reported. Objective: We constructed a continuous optical measurement system to study the dynamics of the PR in a photosensitizer solution containing RBCs. We also measured the relationship between hemolysis and methemoglobin production in the solution. Materials and methods: A 664 nm wavelength continuous laser beam at 60 mW/cm 2 was used to drive the PR, and a broadband (475-650 nm) light beam was used to monitor the absorption spectra during the PR. The light sources were arranged perpendicularly to cross at a 1 × 10 mm cuvette. The sample in this cuvette was prepared from a low-hematocrit rabbit RBC suspension medium containing 30 μg/mL talaporfin sodium, a chlorine photosensitizer. The concentrations of oxygenated hemoglobin, deoxygenated hemoglobin, and methemoglobin were obtained using a multiple regression analysis of the measured spectra. Results: The oxygen saturation decreased continuously during the PR. The relationship between the degree of hemolysis and produced methemoglobin concentration was confirmed. Conclusions: We determined the dynamics of the oxidation and oxygen desorption of hemoglobin, as well as RBC hemolysis, during the PR. Our measurement system, which uses the properties of hemoglobin contained in RBCs, might be useful for continuous monitoring of PR dynamics.

AB - Background: An oxygen-enriched photosensitizer solution was created by the addition of red blood cells (RBCs) as an investigative tool for photosensitization reactions (PRs). Although the oxygen levels and reaction progress can be monitored using the optical characteristics of hemoglobin, previously this has only been done using intermittent measurements. An increase in methemoglobin concentration with irradiation time was reported. Objective: We constructed a continuous optical measurement system to study the dynamics of the PR in a photosensitizer solution containing RBCs. We also measured the relationship between hemolysis and methemoglobin production in the solution. Materials and methods: A 664 nm wavelength continuous laser beam at 60 mW/cm 2 was used to drive the PR, and a broadband (475-650 nm) light beam was used to monitor the absorption spectra during the PR. The light sources were arranged perpendicularly to cross at a 1 × 10 mm cuvette. The sample in this cuvette was prepared from a low-hematocrit rabbit RBC suspension medium containing 30 μg/mL talaporfin sodium, a chlorine photosensitizer. The concentrations of oxygenated hemoglobin, deoxygenated hemoglobin, and methemoglobin were obtained using a multiple regression analysis of the measured spectra. Results: The oxygen saturation decreased continuously during the PR. The relationship between the degree of hemolysis and produced methemoglobin concentration was confirmed. Conclusions: We determined the dynamics of the oxidation and oxygen desorption of hemoglobin, as well as RBC hemolysis, during the PR. Our measurement system, which uses the properties of hemoglobin contained in RBCs, might be useful for continuous monitoring of PR dynamics.

KW - absorption spectrum

KW - hemoglobin

KW - hemolysis

KW - multiple regression analysis

KW - oxygen level

KW - photosensitization reaction

UR - http://www.scopus.com/inward/record.url?scp=85062228810&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85062228810&partnerID=8YFLogxK

U2 - 10.1089/photob.2018.4513

DO - 10.1089/photob.2018.4513

M3 - Article

C2 - 31050934

AN - SCOPUS:85062228810

VL - 37

SP - 110

EP - 116

JO - Photobiomodulation, Photomedicine, and Laser Surgery

JF - Photobiomodulation, Photomedicine, and Laser Surgery

SN - 2578-5478

IS - 2

ER -