Contractile effect of TRPA1 receptor agonists in the isolated mouse intestine

Angelica Penuelas, Kimihito Tashima, Shizuko Tsuchiya, Kenjiro Matsumoto, Tomonori Nakamura, Syunji Horie, Shingo Yano

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

TRPA1 is a member of the transient receptor potential (TRP) channel family expressed in sensory neurons. The present study focused on the effects of TRPA1 activation on contractile responses in isolated mouse intestine preparations. The jejunum, ileum, and proximal and distal colon were surgically isolated from male ddY mice. Intestinal motility was recorded as changes in isotonic tension. TRPA1, TRPM8, and TRPV1 expressions were examined by reverse transcription-polymerase chain reaction (RT-PCR). A TRPA1 agonist allyl isothiocyanate (AITC) dose-dependently induced contractions in the proximal and distal colon, whereas in the jejunum and ileum, even 100 μM AITC caused very little contraction. Likewise, a TRPA1 and TRPM8 agonist icilin, a TRPA1 agonist allicin, and a TRPV1 agonist capsaicin induced contractions in the colon. However, a TRPM8 agonist menthol induced long-lasting relaxation in the colon. Repeated exposure to AITC produced desensitization of its own contraction in the colon. Moreover, contractions induced by AITC generate cross-desensitization with icilin and capsaicin. Tetrodotoxin completely abolished AITC-induced contractions in the colon, whereas atropine significantly attenuated AITC-induced contractions in the distal colon, but not in the proximal colon. Menthol-induced relaxation in the colon was not inhibited by tetrodotoxin and atropine. RT-PCR analysis revealed the expression of TRPA1 and TRPV1, but not TRPM8, throughout the mouse intestine. These results suggest that TRPA1, but not TRPM8, are functionally expressed in the enteric nervous system throughout the mouse intestine on neurons that may also co-express TRPV1, yet the contractile responses to TRPA1 activation differ depending on their location along the intestine.

Original languageEnglish
Pages (from-to)143-150
Number of pages8
JournalEuropean Journal of Pharmacology
Volume576
Issue number1-3
DOIs
Publication statusPublished - 2007 Dec 8
Externally publishedYes

Fingerprint

Intestines
Colon
Menthol
Capsaicin
Tetrodotoxin
Jejunum
Atropine
Ileum
Reverse Transcription
Transient Receptor Potential Channels
Enteric Nervous System
Polymerase Chain Reaction
Gastrointestinal Motility
Sensory Receptor Cells
allyl isothiocyanate
Neurons

Keywords

  • Allyl isothiocyanate
  • Capsaicin
  • Colon
  • Contraction
  • TRPA1
  • TRPV1

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Contractile effect of TRPA1 receptor agonists in the isolated mouse intestine. / Penuelas, Angelica; Tashima, Kimihito; Tsuchiya, Shizuko; Matsumoto, Kenjiro; Nakamura, Tomonori; Horie, Syunji; Yano, Shingo.

In: European Journal of Pharmacology, Vol. 576, No. 1-3, 08.12.2007, p. 143-150.

Research output: Contribution to journalArticle

Penuelas, A, Tashima, K, Tsuchiya, S, Matsumoto, K, Nakamura, T, Horie, S & Yano, S 2007, 'Contractile effect of TRPA1 receptor agonists in the isolated mouse intestine', European Journal of Pharmacology, vol. 576, no. 1-3, pp. 143-150. https://doi.org/10.1016/j.ejphar.2007.08.015
Penuelas, Angelica ; Tashima, Kimihito ; Tsuchiya, Shizuko ; Matsumoto, Kenjiro ; Nakamura, Tomonori ; Horie, Syunji ; Yano, Shingo. / Contractile effect of TRPA1 receptor agonists in the isolated mouse intestine. In: European Journal of Pharmacology. 2007 ; Vol. 576, No. 1-3. pp. 143-150.
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