Contrasting action of IL-12 and IL-18 in the development of dextran sodium sulphate colitis in mice

H. Takagi, Takanori Kanai, A. Okazawa, Y. Kishi, Toshiro Sato, Hiromasa Takaishi, Nagamu Inoue, Haruhiko Ogata, Yasushi Iwao, K. Hoshino, K. Takeda, S. Akira, M. Watanabe, H. Ishii, Toshifumi Hibi

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Background: Interleukin (IL)-12 and IL-18 are major interferon (IFN)-γ-inducing factors that collaborate with each other. The present study was conducted to determine the distinct roles of IL-12 and IL-18 in the development of dextran sulphate sodium (DSS) colitis in mice. Methods: Colitis was induced in IL-12p35-/-, IL-18-/-, IL-18 receptor -/- and control mice with DSS. Clinical and histopathological analysis was conducted using survival rate, weight loss score, diarrhoea score, bloody stool score and histological score. In addition, cytokine production by lamina propria mononuclear cells (LPMCs) was examined using the specific enzyme-linked immunoassay. Results: IL-12p35-/- mice developed only a mild disease associated with no lethality and few histopathological abnormalities. In contrast, IL-18-/- and IL-18R-/- mice developed more severe colitis associated with high lethality and more histopathological abnormalities compared with control mice. LPMCs from DSS-fed IL-18-/- mice produced significantly higher amounts of IFN-γ, while LPMCs from DSS-fed IL-12-/- mice produced lower amounts of IFN-γ and tumour necrosis factor (TNF)-α compared with control mice. Conclusion: These results suggest that IL-18 might function with manners different from IL-12 at some pathological conditions in the development of colitis.

Original languageEnglish
Pages (from-to)837-844
Number of pages8
JournalScandinavian Journal of Gastroenterology
Volume38
Issue number8
DOIs
Publication statusPublished - 2003 Aug 1
Externally publishedYes

Fingerprint

Dextran Sulfate
Interleukin-18
Colitis
Interleukin-12
Interleukins
Interferons
Mucous Membrane
Interleukin-18 Receptors
Immunoenzyme Techniques
Weight Loss
Diarrhea
Tumor Necrosis Factor-alpha
Cytokines

Keywords

  • DSS
  • IL-12
  • IL-18
  • Murine colitis

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Contrasting action of IL-12 and IL-18 in the development of dextran sodium sulphate colitis in mice. / Takagi, H.; Kanai, Takanori; Okazawa, A.; Kishi, Y.; Sato, Toshiro; Takaishi, Hiromasa; Inoue, Nagamu; Ogata, Haruhiko; Iwao, Yasushi; Hoshino, K.; Takeda, K.; Akira, S.; Watanabe, M.; Ishii, H.; Hibi, Toshifumi.

In: Scandinavian Journal of Gastroenterology, Vol. 38, No. 8, 01.08.2003, p. 837-844.

Research output: Contribution to journalArticle

Takagi, H. ; Kanai, Takanori ; Okazawa, A. ; Kishi, Y. ; Sato, Toshiro ; Takaishi, Hiromasa ; Inoue, Nagamu ; Ogata, Haruhiko ; Iwao, Yasushi ; Hoshino, K. ; Takeda, K. ; Akira, S. ; Watanabe, M. ; Ishii, H. ; Hibi, Toshifumi. / Contrasting action of IL-12 and IL-18 in the development of dextran sodium sulphate colitis in mice. In: Scandinavian Journal of Gastroenterology. 2003 ; Vol. 38, No. 8. pp. 837-844.
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AU - Kishi, Y.

AU - Sato, Toshiro

AU - Takaishi, Hiromasa

AU - Inoue, Nagamu

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AU - Hoshino, K.

AU - Takeda, K.

AU - Akira, S.

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AU - Ishii, H.

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