Abstract
Background: Interleukin (IL)-12 and IL-18 are major interferon (IFN)-γ-inducing factors that collaborate with each other. The present study was conducted to determine the distinct roles of IL-12 and IL-18 in the development of dextran sulphate sodium (DSS) colitis in mice. Methods: Colitis was induced in IL-12p35-/-, IL-18-/-, IL-18 receptor -/- and control mice with DSS. Clinical and histopathological analysis was conducted using survival rate, weight loss score, diarrhoea score, bloody stool score and histological score. In addition, cytokine production by lamina propria mononuclear cells (LPMCs) was examined using the specific enzyme-linked immunoassay. Results: IL-12p35-/- mice developed only a mild disease associated with no lethality and few histopathological abnormalities. In contrast, IL-18-/- and IL-18R-/- mice developed more severe colitis associated with high lethality and more histopathological abnormalities compared with control mice. LPMCs from DSS-fed IL-18-/- mice produced significantly higher amounts of IFN-γ, while LPMCs from DSS-fed IL-12-/- mice produced lower amounts of IFN-γ and tumour necrosis factor (TNF)-α compared with control mice. Conclusion: These results suggest that IL-18 might function with manners different from IL-12 at some pathological conditions in the development of colitis.
| Original language | English |
|---|---|
| Pages (from-to) | 837-844 |
| Number of pages | 8 |
| Journal | Scandinavian Journal of Gastroenterology |
| Volume | 38 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2003 Aug 1 |
| Externally published | Yes |
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Keywords
- DSS
- IL-12
- IL-18
- Murine colitis
ASJC Scopus subject areas
- Gastroenterology
Cite this
Contrasting action of IL-12 and IL-18 in the development of dextran sodium sulphate colitis in mice. / Takagi, H.; Kanai, Takanori; Okazawa, A.; Kishi, Y.; Sato, Toshiro; Takaishi, Hiromasa; Inoue, Nagamu; Ogata, Haruhiko; Iwao, Yasushi; Hoshino, K.; Takeda, K.; Akira, S.; Watanabe, M.; Ishii, H.; Hibi, Toshifumi.
In: Scandinavian Journal of Gastroenterology, Vol. 38, No. 8, 01.08.2003, p. 837-844.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Contrasting action of IL-12 and IL-18 in the development of dextran sodium sulphate colitis in mice
AU - Takagi, H.
AU - Kanai, Takanori
AU - Okazawa, A.
AU - Kishi, Y.
AU - Sato, Toshiro
AU - Takaishi, Hiromasa
AU - Inoue, Nagamu
AU - Ogata, Haruhiko
AU - Iwao, Yasushi
AU - Hoshino, K.
AU - Takeda, K.
AU - Akira, S.
AU - Watanabe, M.
AU - Ishii, H.
AU - Hibi, Toshifumi
PY - 2003/8/1
Y1 - 2003/8/1
N2 - Background: Interleukin (IL)-12 and IL-18 are major interferon (IFN)-γ-inducing factors that collaborate with each other. The present study was conducted to determine the distinct roles of IL-12 and IL-18 in the development of dextran sulphate sodium (DSS) colitis in mice. Methods: Colitis was induced in IL-12p35-/-, IL-18-/-, IL-18 receptor -/- and control mice with DSS. Clinical and histopathological analysis was conducted using survival rate, weight loss score, diarrhoea score, bloody stool score and histological score. In addition, cytokine production by lamina propria mononuclear cells (LPMCs) was examined using the specific enzyme-linked immunoassay. Results: IL-12p35-/- mice developed only a mild disease associated with no lethality and few histopathological abnormalities. In contrast, IL-18-/- and IL-18R-/- mice developed more severe colitis associated with high lethality and more histopathological abnormalities compared with control mice. LPMCs from DSS-fed IL-18-/- mice produced significantly higher amounts of IFN-γ, while LPMCs from DSS-fed IL-12-/- mice produced lower amounts of IFN-γ and tumour necrosis factor (TNF)-α compared with control mice. Conclusion: These results suggest that IL-18 might function with manners different from IL-12 at some pathological conditions in the development of colitis.
AB - Background: Interleukin (IL)-12 and IL-18 are major interferon (IFN)-γ-inducing factors that collaborate with each other. The present study was conducted to determine the distinct roles of IL-12 and IL-18 in the development of dextran sulphate sodium (DSS) colitis in mice. Methods: Colitis was induced in IL-12p35-/-, IL-18-/-, IL-18 receptor -/- and control mice with DSS. Clinical and histopathological analysis was conducted using survival rate, weight loss score, diarrhoea score, bloody stool score and histological score. In addition, cytokine production by lamina propria mononuclear cells (LPMCs) was examined using the specific enzyme-linked immunoassay. Results: IL-12p35-/- mice developed only a mild disease associated with no lethality and few histopathological abnormalities. In contrast, IL-18-/- and IL-18R-/- mice developed more severe colitis associated with high lethality and more histopathological abnormalities compared with control mice. LPMCs from DSS-fed IL-18-/- mice produced significantly higher amounts of IFN-γ, while LPMCs from DSS-fed IL-12-/- mice produced lower amounts of IFN-γ and tumour necrosis factor (TNF)-α compared with control mice. Conclusion: These results suggest that IL-18 might function with manners different from IL-12 at some pathological conditions in the development of colitis.
KW - DSS
KW - IL-12
KW - IL-18
KW - Murine colitis
UR - http://www.scopus.com/inward/record.url?scp=0041426439&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0041426439&partnerID=8YFLogxK
U2 - 10.1080/00365520310004047
DO - 10.1080/00365520310004047
M3 - Article
VL - 38
SP - 837
EP - 844
JO - Scandinavian Journal of Gastroenterology
JF - Scandinavian Journal of Gastroenterology
SN - 0036-5521
IS - 8
ER -