Contribution of matrilysin (MMP-7) to the metastatic pathway of human colorectal cancers

Y. Adachi, H. Yamamoto, F. Itoh, Y. Hinoda, Y. Okada, K. Imai

Research output: Contribution to journalArticle

182 Citations (Scopus)

Abstract

Background/Aim - Matrilysin is one of the matrix metalloproteinases that has a critical role in tumour invasions and is often expressed in gastrointestinal cancers. The aim of this study was to examine the role of matrilysin in metastasis of human colorectal cancers. Patients (Subjects)/Methods - The relation between matrilysin expression and Dukes's type was investigated immunohistochemically in 83 surgically resected colorectal cancers, including five with liver metastasis. Moreover, the effects of matrilysin on the in vivo invasive and metastatic potential of colon cancer cells transfected with matrilysin cDNA were examined after subcutaneous injection into SCID mice. Results - In 46% of primary and all of metastatic liver tumours, over 10% of cancer cells were stained positively for matrilysin. The expression of matrilysin correlated significantly with the presence of nodal or distant metastases (p<0.05). In addition, matrilysin transfectants formed invasive tumours and multiple liver metastases in SCID mice, without producing any significant difference in the subcutaneous tumour growth from mock transfectants. Casein zymography showed that the invading and metastasised tumours showed conspicuous matrilysin activity, which correlated with the number of metastatic lesions (p<0.001). Conclusions - Matrilysin showed a correlation with metastasis in a cohort of 83 colorectal cancer patients and marked metastatic potentiation in human colorectal cancer xenografts, indicating that it may play a critical role in the metastatic pathway of colorectal cancers.

Original languageEnglish
Pages (from-to)252-258
Number of pages7
JournalGut
Volume45
Issue number2
Publication statusPublished - 1999

Fingerprint

Matrix Metalloproteinase 7
Matrix Metalloproteinases
Colorectal Neoplasms
Neoplasm Metastasis
Neoplasms
SCID Mice
Liver
Gastrointestinal Neoplasms
Subcutaneous Injections
Caseins
Heterografts
Colonic Neoplasms
Complementary DNA

Keywords

  • Colorectal cancer
  • Human
  • Matrilysin
  • Matrix metalloproteinase
  • Metastasis
  • MMP-7
  • Transfection

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Adachi, Y., Yamamoto, H., Itoh, F., Hinoda, Y., Okada, Y., & Imai, K. (1999). Contribution of matrilysin (MMP-7) to the metastatic pathway of human colorectal cancers. Gut, 45(2), 252-258.

Contribution of matrilysin (MMP-7) to the metastatic pathway of human colorectal cancers. / Adachi, Y.; Yamamoto, H.; Itoh, F.; Hinoda, Y.; Okada, Y.; Imai, K.

In: Gut, Vol. 45, No. 2, 1999, p. 252-258.

Research output: Contribution to journalArticle

Adachi, Y, Yamamoto, H, Itoh, F, Hinoda, Y, Okada, Y & Imai, K 1999, 'Contribution of matrilysin (MMP-7) to the metastatic pathway of human colorectal cancers', Gut, vol. 45, no. 2, pp. 252-258.
Adachi Y, Yamamoto H, Itoh F, Hinoda Y, Okada Y, Imai K. Contribution of matrilysin (MMP-7) to the metastatic pathway of human colorectal cancers. Gut. 1999;45(2):252-258.
Adachi, Y. ; Yamamoto, H. ; Itoh, F. ; Hinoda, Y. ; Okada, Y. ; Imai, K. / Contribution of matrilysin (MMP-7) to the metastatic pathway of human colorectal cancers. In: Gut. 1999 ; Vol. 45, No. 2. pp. 252-258.
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