Control of cell cycle regulatory proteins and modulation of STAT1 proteins by IFN-γ in human prostatic JCA-1 cells

Mototsugu Oya, S. Konno, Y. Chen, H. Tazaki, C. Mallouh, J. M. Wu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The addition of human recombinant IFN-γ (102 and 103 IU/ml) inhibited human prostatic JCA-1 cell growth by 27% and 64%, respectively. Since the high dose of IFN-γ elicited an increase in G1 concomitant with a decrease in G2/M phases of the cell cycle, changes in the expression of cell cycle regulatory protein molecules were analyzed by Western blots. Results of these experiments show that IFN-γ down regulated the G1/S transition molecules, e.g., cyclin D1, the cyclin-dependent protein kinase Cdk4, and the retinoblastoma gene product (pRB), but increased the G2/M transition molecules, e.g., cyclin B1 and p34(cdc2) (Cdk1). Possible modulation of Cdk-inhibitors (CDKIs), e.g., p53 and p21(WAF1), which have checkpoint functions in the cell cycle, by IFN-γ, was also studied. The p53 was induced by both 102 and 103 IU/ml IFN-γ. At 103 IU/ml, IFN-γ inhibited p21(WAF1) increased the expression of STAT1α, and sustained the elevated STAT1α for up to 96 h. Thus several mechanisms may be involved in the antiproliferative effects of IFN-γ.

Original languageEnglish
Pages (from-to)835-839
Number of pages5
JournalInternational Journal of Oncology
Volume10
Issue number4
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

STAT1 Transcription Factor
Cell Cycle Proteins
Cell Cycle
Retinoblastoma Genes
Cyclin B1
Cyclin-Dependent Kinases
G2 Phase
Cyclin D1
Cell Division
Western Blotting
Growth

Keywords

  • Cyclin-dependent kinase inhibitors
  • Cyclin-dependent kinases
  • Cyclins
  • IFN-γ
  • Prostate cancer
  • STAT1

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Control of cell cycle regulatory proteins and modulation of STAT1 proteins by IFN-γ in human prostatic JCA-1 cells. / Oya, Mototsugu; Konno, S.; Chen, Y.; Tazaki, H.; Mallouh, C.; Wu, J. M.

In: International Journal of Oncology, Vol. 10, No. 4, 1997, p. 835-839.

Research output: Contribution to journalArticle

Oya, Mototsugu ; Konno, S. ; Chen, Y. ; Tazaki, H. ; Mallouh, C. ; Wu, J. M. / Control of cell cycle regulatory proteins and modulation of STAT1 proteins by IFN-γ in human prostatic JCA-1 cells. In: International Journal of Oncology. 1997 ; Vol. 10, No. 4. pp. 835-839.
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AU - Wu, J. M.

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