Control of cell death by the smart polymeric vehicle

Keiji Fujimoto, Chizu Iwasaki, Chigusa Arai, Masayuki Kuwako, Etsuko Yasugi

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

We report a novel drug delivery system for apoptosis induction by a "smart" polymer vehicle possessing thermosensitivity and bioaffinity. The polymer chain was prepared by copolymerization of N-isopropyl-acrylamide and N-methacryloyloxysuccinimide. Cell-adhesive RGDS peptide was conjugated with the copolymer as a ligand model for bioaffinity. When the temperature was increased, nanoscale aggregates precipitated from a copolymer aqueous solution. Either dolichyl phosphate (dol-p), which is an apoptotic inducer, or dolichol was added to aggregates at around the precipitation temperature (31°C), and the temperature was raised to 37°C for incorporation. Aggregates incorporating dol-p or dolicol were added to a human promonocytic leukemia U937 cell suspension at 37°C. When the temperature was lowered to 25 °C, cells underwent apoptosis in the presence of Ca2+. Probably, copolymer vehicles were concentrated on a cell surface through the binding of RGDS and integrin and the release of lipid inducers was caused by the disruption of vehicles in response to temperature.

Original languageEnglish
Pages (from-to)515-518
Number of pages4
JournalBiomacromolecules
Volume1
Issue number4
DOIs
Publication statusPublished - 2000 Jan 1

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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