Control of cellular activity of fibroblasts on size-tuned fibrous hydroxyapatite nanocrystals

Shinnosuke Okada, Atsushi Nagai, Yuya Oaki, Jun Komotori, Hiroaki Imai

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We controlled the performance of L929 mouse fibroblasts using various hydroxyapatite (HA) nanocrystals, such as nanofibers, nanoneedles, and nanosheets, to better understand the effects of size and shape of the HA nanocrystals on the cells. The cellular activity on nanofibers with a diameter of 50-100 nm was significantly enhanced relative to that on a flat HA surface because large amounts of the proteins needed for adhesion and proliferation could be stored in the substrate. On the other hand, initial adhesion and subsequent proliferation were inhibited on surfaces consisting of fine nanoneedles and nanosheets with a diameter/thickness of less than 30 nm due to the limited area available for the formation of focal adhesions. These facts indicate that fibroblast activity is highly sensitive to the surface topography. Therefore, size tuning of the nanoscale units composing the substrate is essential to enhance cellular performance.

Original languageEnglish
Pages (from-to)1290-1297
Number of pages8
JournalActa Biomaterialia
Volume7
Issue number3
DOIs
Publication statusPublished - 2011 Mar

Fingerprint

Durapatite
Fibroblasts
Hydroxyapatite
Nanoneedles
Nanoparticles
Nanocrystals
Nanofibers
Adhesion
Nanosheets
Focal Adhesions
Surface topography
Substrates
Tuning
Proteins

Keywords

  • Cell adhesion
  • Cell proliferation
  • Fibroblasts
  • Hydroxyapatite
  • Nanotopography

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

Control of cellular activity of fibroblasts on size-tuned fibrous hydroxyapatite nanocrystals. / Okada, Shinnosuke; Nagai, Atsushi; Oaki, Yuya; Komotori, Jun; Imai, Hiroaki.

In: Acta Biomaterialia, Vol. 7, No. 3, 03.2011, p. 1290-1297.

Research output: Contribution to journalArticle

@article{bfa2d5bb69174567bbac386c4090890d,
title = "Control of cellular activity of fibroblasts on size-tuned fibrous hydroxyapatite nanocrystals",
abstract = "We controlled the performance of L929 mouse fibroblasts using various hydroxyapatite (HA) nanocrystals, such as nanofibers, nanoneedles, and nanosheets, to better understand the effects of size and shape of the HA nanocrystals on the cells. The cellular activity on nanofibers with a diameter of 50-100 nm was significantly enhanced relative to that on a flat HA surface because large amounts of the proteins needed for adhesion and proliferation could be stored in the substrate. On the other hand, initial adhesion and subsequent proliferation were inhibited on surfaces consisting of fine nanoneedles and nanosheets with a diameter/thickness of less than 30 nm due to the limited area available for the formation of focal adhesions. These facts indicate that fibroblast activity is highly sensitive to the surface topography. Therefore, size tuning of the nanoscale units composing the substrate is essential to enhance cellular performance.",
keywords = "Cell adhesion, Cell proliferation, Fibroblasts, Hydroxyapatite, Nanotopography",
author = "Shinnosuke Okada and Atsushi Nagai and Yuya Oaki and Jun Komotori and Hiroaki Imai",
year = "2011",
month = "3",
doi = "10.1016/j.actbio.2010.10.010",
language = "English",
volume = "7",
pages = "1290--1297",
journal = "Acta Biomaterialia",
issn = "1742-7061",
publisher = "Elsevier BV",
number = "3",

}

TY - JOUR

T1 - Control of cellular activity of fibroblasts on size-tuned fibrous hydroxyapatite nanocrystals

AU - Okada, Shinnosuke

AU - Nagai, Atsushi

AU - Oaki, Yuya

AU - Komotori, Jun

AU - Imai, Hiroaki

PY - 2011/3

Y1 - 2011/3

N2 - We controlled the performance of L929 mouse fibroblasts using various hydroxyapatite (HA) nanocrystals, such as nanofibers, nanoneedles, and nanosheets, to better understand the effects of size and shape of the HA nanocrystals on the cells. The cellular activity on nanofibers with a diameter of 50-100 nm was significantly enhanced relative to that on a flat HA surface because large amounts of the proteins needed for adhesion and proliferation could be stored in the substrate. On the other hand, initial adhesion and subsequent proliferation were inhibited on surfaces consisting of fine nanoneedles and nanosheets with a diameter/thickness of less than 30 nm due to the limited area available for the formation of focal adhesions. These facts indicate that fibroblast activity is highly sensitive to the surface topography. Therefore, size tuning of the nanoscale units composing the substrate is essential to enhance cellular performance.

AB - We controlled the performance of L929 mouse fibroblasts using various hydroxyapatite (HA) nanocrystals, such as nanofibers, nanoneedles, and nanosheets, to better understand the effects of size and shape of the HA nanocrystals on the cells. The cellular activity on nanofibers with a diameter of 50-100 nm was significantly enhanced relative to that on a flat HA surface because large amounts of the proteins needed for adhesion and proliferation could be stored in the substrate. On the other hand, initial adhesion and subsequent proliferation were inhibited on surfaces consisting of fine nanoneedles and nanosheets with a diameter/thickness of less than 30 nm due to the limited area available for the formation of focal adhesions. These facts indicate that fibroblast activity is highly sensitive to the surface topography. Therefore, size tuning of the nanoscale units composing the substrate is essential to enhance cellular performance.

KW - Cell adhesion

KW - Cell proliferation

KW - Fibroblasts

KW - Hydroxyapatite

KW - Nanotopography

UR - http://www.scopus.com/inward/record.url?scp=79251622427&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79251622427&partnerID=8YFLogxK

U2 - 10.1016/j.actbio.2010.10.010

DO - 10.1016/j.actbio.2010.10.010

M3 - Article

C2 - 20965284

AN - SCOPUS:79251622427

VL - 7

SP - 1290

EP - 1297

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

IS - 3

ER -