Control of cellular organization around collagen beads using dielectrophoresis

Shogo Miyata, Yu Sugimoto

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Tissues formed by cells seeded in hydrogels are used for biotechnology, cell-based assays, and tissue engineering. We have previously presented a cell-micropatterning technique that localizes live cells within hydrogels using dielectrophoretic (DEP) forces, and have demonstrated the ability to modulate tissue function through the control of microscale cell architecture. In this study, we developed a novel cell patterning technology for accumulating cells around collagen micro-beads. As a case study, we produce collagen-alginate beads for scaffold and accumulate chondrocytes around the micro-beads by combining DEP force and flow of the buffer solution. This approach enables an expanded range of studies examining the influence of 3D cellular architecture on micro-scale levels.

Original languageEnglish
Title of host publication2010 World Automation Congress, WAC 2010
Publication statusPublished - 2010 Dec 1
Event2010 World Automation Congress, WAC 2010 - Kobe, Japan
Duration: 2010 Sep 192010 Sep 23

Publication series

Name2010 World Automation Congress, WAC 2010

Other

Other2010 World Automation Congress, WAC 2010
CountryJapan
CityKobe
Period10/9/1910/9/23

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Keywords

  • Alginate
  • Cellular organization
  • Collagen
  • Dielectrophoresis

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Miyata, S., & Sugimoto, Y. (2010). Control of cellular organization around collagen beads using dielectrophoresis. In 2010 World Automation Congress, WAC 2010 [5665320] (2010 World Automation Congress, WAC 2010).