Abstract
Tissues formed by cells seeded in hydrogels are used in biotechnology, cell-based assays, and tissue engineering. We previously presented a cell micro-patterning technique that localizes live cells within hydrogels using dielectrophoretic (DEP) forces. We also demonstrated the ability to modulate tissue function through the control of microscale cell architecture. In this study, we developed a novel cell patterning technology for accumulating cells around collagen microbeads using DEP forces. As a case study, we produced collagen-alginate microbeads as a cell-adhesive scaffold and accumulated bovine chondrocytes to cover the microbeads by combining the DEP forces with a flow of buffer solution. This approach allows studies to better examine the influence of three-dimensional cellular architecture on microscale levels.
| Original language | English |
|---|---|
| Pages (from-to) | 177-186 |
| Number of pages | 10 |
| Journal | Intelligent Automation and Soft Computing |
| Volume | 18 |
| Issue number | 2 |
| Publication status | Published - 2012 |
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Keywords
- Cell patterning
- Cellular organization
- Dielectrophoresis
- Hydrogel microbeads
ASJC Scopus subject areas
- Artificial Intelligence
- Software
- Theoretical Computer Science
- Computational Theory and Mathematics
Cite this
Control of cellular organization around collagen beads using dielectrophoresis. / Miyata, Shogo; Sugimoto, Yu.
In: Intelligent Automation and Soft Computing, Vol. 18, No. 2, 2012, p. 177-186.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Control of cellular organization around collagen beads using dielectrophoresis
AU - Miyata, Shogo
AU - Sugimoto, Yu
PY - 2012
Y1 - 2012
N2 - Tissues formed by cells seeded in hydrogels are used in biotechnology, cell-based assays, and tissue engineering. We previously presented a cell micro-patterning technique that localizes live cells within hydrogels using dielectrophoretic (DEP) forces. We also demonstrated the ability to modulate tissue function through the control of microscale cell architecture. In this study, we developed a novel cell patterning technology for accumulating cells around collagen microbeads using DEP forces. As a case study, we produced collagen-alginate microbeads as a cell-adhesive scaffold and accumulated bovine chondrocytes to cover the microbeads by combining the DEP forces with a flow of buffer solution. This approach allows studies to better examine the influence of three-dimensional cellular architecture on microscale levels.
AB - Tissues formed by cells seeded in hydrogels are used in biotechnology, cell-based assays, and tissue engineering. We previously presented a cell micro-patterning technique that localizes live cells within hydrogels using dielectrophoretic (DEP) forces. We also demonstrated the ability to modulate tissue function through the control of microscale cell architecture. In this study, we developed a novel cell patterning technology for accumulating cells around collagen microbeads using DEP forces. As a case study, we produced collagen-alginate microbeads as a cell-adhesive scaffold and accumulated bovine chondrocytes to cover the microbeads by combining the DEP forces with a flow of buffer solution. This approach allows studies to better examine the influence of three-dimensional cellular architecture on microscale levels.
KW - Cell patterning
KW - Cellular organization
KW - Dielectrophoresis
KW - Hydrogel microbeads
UR - http://www.scopus.com/inward/record.url?scp=84862511580&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84862511580&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:84862511580
VL - 18
SP - 177
EP - 186
JO - Intelligent Automation and Soft Computing
JF - Intelligent Automation and Soft Computing
SN - 1079-8587
IS - 2
ER -