Control of cellular organization around collagen beads using dielectrophoresis

Shogo Miyata, Yu Sugimoto

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Tissues formed by cells seeded in hydrogels are used in biotechnology, cell-based assays, and tissue engineering. We previously presented a cell micro-patterning technique that localizes live cells within hydrogels using dielectrophoretic (DEP) forces. We also demonstrated the ability to modulate tissue function through the control of microscale cell architecture. In this study, we developed a novel cell patterning technology for accumulating cells around collagen microbeads using DEP forces. As a case study, we produced collagen-alginate microbeads as acell-adhesive scaffold and accumulated bovine chondrocytes to cover the microbeads by combining the DEP forces with a flow of buffer solution. This approach allows studies to better examine the influence ofthree-dimensional cellular architecture on microscale levels.

Original languageEnglish
Pages (from-to)177-186
Number of pages10
JournalIntelligent Automation and Soft Computing
Volume18
Issue number2
DOIs
Publication statusPublished - 2012 Jan

Keywords

  • Cell patterning
  • Cellular Organization
  • Dielectrophoresis
  • Hydrogel microbeads

ASJC Scopus subject areas

  • Software
  • Theoretical Computer Science
  • Computational Theory and Mathematics
  • Artificial Intelligence

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