Control of cellular organization around collagen beads using dielectrophoresis

Shogo Miyata, Yu Sugimoto

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Tissues formed by cells seeded in hydrogels are used in biotechnology, cell-based assays, and tissue engineering. We previously presented a cell micro-patterning technique that localizes live cells within hydrogels using dielectrophoretic (DEP) forces. We also demonstrated the ability to modulate tissue function through the control of microscale cell architecture. In this study, we developed a novel cell patterning technology for accumulating cells around collagen microbeads using DEP forces. As a case study, we produced collagen-alginate microbeads as a cell-adhesive scaffold and accumulated bovine chondrocytes to cover the microbeads by combining the DEP forces with a flow of buffer solution. This approach allows studies to better examine the influence of three-dimensional cellular architecture on microscale levels.

Original languageEnglish
Pages (from-to)177-186
Number of pages10
JournalIntelligent Automation and Soft Computing
Volume18
Issue number2
Publication statusPublished - 2012

Fingerprint

Dielectrophoresis
Collagen
Electrophoresis
Hydrogels
Tissue
Alginate
Cell
Scaffolds (biology)
Biotechnology
Tissue engineering
Assays
Adhesives
Hydrogel
Patterning
Tissue Engineering
Scaffold
Buffer
Cover
Three-dimensional

Keywords

  • Cell patterning
  • Cellular organization
  • Dielectrophoresis
  • Hydrogel microbeads

ASJC Scopus subject areas

  • Artificial Intelligence
  • Software
  • Theoretical Computer Science
  • Computational Theory and Mathematics

Cite this

Control of cellular organization around collagen beads using dielectrophoresis. / Miyata, Shogo; Sugimoto, Yu.

In: Intelligent Automation and Soft Computing, Vol. 18, No. 2, 2012, p. 177-186.

Research output: Contribution to journalArticle

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