Control of the cell death pathway by Dapaf-1, a Drosophila Apaf-1/CED-4-related caspase activator

Hirotaka Kanuka, Kazunobu Sawamoto, Naohiro Inohara, Kenji Matsuno, Hideyuki Okano, Masayuki Miura

Research output: Contribution to journalArticle

209 Citations (Scopus)

Abstract

We identified a Drosophila Apaf-1/CED-4 homolog gene, dapaf-1. Alternative splicing results in two dapaf-1 mRNA species, which encode distinct forms of caspase activator, Dapaf-1L (Apaf-1 type) and Dapaf-1S (CED-4 type). Distinct caspases were activated by these Dapaf-1 isoforms. Loss of Dapaf-1 function resulted in defective cytochrome c-dependent caspase activities and reduced apoptosis in embryo and in larval brain. Dapaf-1 activities were also involved in cell death induced by ectopic expression of reaper in the compound eye. These data suggest that Dapaf-1/cytochrome c-dependent cell death-inducing machinery is present in Drosophila, and the requirement of Dapaf-1/Apaf-1 in neural cell death is conserved through evolution.

Original languageEnglish
Pages (from-to)757-769
Number of pages13
JournalMolecular Cell
Volume4
Issue number5
Publication statusPublished - 1999 Nov
Externally publishedYes

Fingerprint

Caspases
Drosophila
Cell Death
Cytochromes c1
Alternative Splicing
Cytochromes c
Protein Isoforms
Embryonic Structures
Apoptosis
Messenger RNA
Brain
Genes

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Kanuka, H., Sawamoto, K., Inohara, N., Matsuno, K., Okano, H., & Miura, M. (1999). Control of the cell death pathway by Dapaf-1, a Drosophila Apaf-1/CED-4-related caspase activator. Molecular Cell, 4(5), 757-769.

Control of the cell death pathway by Dapaf-1, a Drosophila Apaf-1/CED-4-related caspase activator. / Kanuka, Hirotaka; Sawamoto, Kazunobu; Inohara, Naohiro; Matsuno, Kenji; Okano, Hideyuki; Miura, Masayuki.

In: Molecular Cell, Vol. 4, No. 5, 11.1999, p. 757-769.

Research output: Contribution to journalArticle

Kanuka, H, Sawamoto, K, Inohara, N, Matsuno, K, Okano, H & Miura, M 1999, 'Control of the cell death pathway by Dapaf-1, a Drosophila Apaf-1/CED-4-related caspase activator', Molecular Cell, vol. 4, no. 5, pp. 757-769.
Kanuka, Hirotaka ; Sawamoto, Kazunobu ; Inohara, Naohiro ; Matsuno, Kenji ; Okano, Hideyuki ; Miura, Masayuki. / Control of the cell death pathway by Dapaf-1, a Drosophila Apaf-1/CED-4-related caspase activator. In: Molecular Cell. 1999 ; Vol. 4, No. 5. pp. 757-769.
@article{216cb8e7484c4e21bf16bbd71390eda9,
title = "Control of the cell death pathway by Dapaf-1, a Drosophila Apaf-1/CED-4-related caspase activator",
abstract = "We identified a Drosophila Apaf-1/CED-4 homolog gene, dapaf-1. Alternative splicing results in two dapaf-1 mRNA species, which encode distinct forms of caspase activator, Dapaf-1L (Apaf-1 type) and Dapaf-1S (CED-4 type). Distinct caspases were activated by these Dapaf-1 isoforms. Loss of Dapaf-1 function resulted in defective cytochrome c-dependent caspase activities and reduced apoptosis in embryo and in larval brain. Dapaf-1 activities were also involved in cell death induced by ectopic expression of reaper in the compound eye. These data suggest that Dapaf-1/cytochrome c-dependent cell death-inducing machinery is present in Drosophila, and the requirement of Dapaf-1/Apaf-1 in neural cell death is conserved through evolution.",
author = "Hirotaka Kanuka and Kazunobu Sawamoto and Naohiro Inohara and Kenji Matsuno and Hideyuki Okano and Masayuki Miura",
year = "1999",
month = "11",
language = "English",
volume = "4",
pages = "757--769",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "5",

}

TY - JOUR

T1 - Control of the cell death pathway by Dapaf-1, a Drosophila Apaf-1/CED-4-related caspase activator

AU - Kanuka, Hirotaka

AU - Sawamoto, Kazunobu

AU - Inohara, Naohiro

AU - Matsuno, Kenji

AU - Okano, Hideyuki

AU - Miura, Masayuki

PY - 1999/11

Y1 - 1999/11

N2 - We identified a Drosophila Apaf-1/CED-4 homolog gene, dapaf-1. Alternative splicing results in two dapaf-1 mRNA species, which encode distinct forms of caspase activator, Dapaf-1L (Apaf-1 type) and Dapaf-1S (CED-4 type). Distinct caspases were activated by these Dapaf-1 isoforms. Loss of Dapaf-1 function resulted in defective cytochrome c-dependent caspase activities and reduced apoptosis in embryo and in larval brain. Dapaf-1 activities were also involved in cell death induced by ectopic expression of reaper in the compound eye. These data suggest that Dapaf-1/cytochrome c-dependent cell death-inducing machinery is present in Drosophila, and the requirement of Dapaf-1/Apaf-1 in neural cell death is conserved through evolution.

AB - We identified a Drosophila Apaf-1/CED-4 homolog gene, dapaf-1. Alternative splicing results in two dapaf-1 mRNA species, which encode distinct forms of caspase activator, Dapaf-1L (Apaf-1 type) and Dapaf-1S (CED-4 type). Distinct caspases were activated by these Dapaf-1 isoforms. Loss of Dapaf-1 function resulted in defective cytochrome c-dependent caspase activities and reduced apoptosis in embryo and in larval brain. Dapaf-1 activities were also involved in cell death induced by ectopic expression of reaper in the compound eye. These data suggest that Dapaf-1/cytochrome c-dependent cell death-inducing machinery is present in Drosophila, and the requirement of Dapaf-1/Apaf-1 in neural cell death is conserved through evolution.

UR - http://www.scopus.com/inward/record.url?scp=0033231614&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033231614&partnerID=8YFLogxK

M3 - Article

VL - 4

SP - 757

EP - 769

JO - Molecular Cell

JF - Molecular Cell

SN - 1097-2765

IS - 5

ER -