Controlled gene expression in primary Lgr5 organoid cultures

Bon Kyoung Koo, Daniel E. Stange, Toshiro Sato, Wouter Karthaus, Henner F. Farin, Meritxell Huch, Johan H. Van Es, Hans Clevers

Research output: Contribution to journalArticle

178 Citations (Scopus)

Abstract

The study of gene function in endodermal epithelia such as of stomach, small intestine and colon relies heavily on transgenic approaches. Establishing such animal models is laborious, expensive and time-consuming. We present here a method based on Cre recombinaseinducible retrovirus vectors that allows the conditional manipulation of gene expression in primary mouse organoid culture systems.

Original languageEnglish
Pages (from-to)81-83
Number of pages3
JournalNature Methods
Volume9
Issue number1
DOIs
Publication statusPublished - 2012 Jan 1

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Koo, B. K., Stange, D. E., Sato, T., Karthaus, W., Farin, H. F., Huch, M., Van Es, J. H., & Clevers, H. (2012). Controlled gene expression in primary Lgr5 organoid cultures. Nature Methods, 9(1), 81-83. https://doi.org/10.1038/nmeth.1802