Controlled gene expression in primary Lgr5 organoid cultures

Bon Kyoung Koo, Daniel E. Stange, Toshiro Sato, Wouter Karthaus, Henner F. Farin, Meritxell Huch, Johan H. Van Es, Hans Clevers

Research output: Contribution to journalArticle

169 Citations (Scopus)

Abstract

The study of gene function in endodermal epithelia such as of stomach, small intestine and colon relies heavily on transgenic approaches. Establishing such animal models is laborious, expensive and time-consuming. We present here a method based on Cre recombinaseinducible retrovirus vectors that allows the conditional manipulation of gene expression in primary mouse organoid culture systems.

Original languageEnglish
Pages (from-to)81-83
Number of pages3
JournalNature Methods
Volume9
Issue number1
DOIs
Publication statusPublished - 2012 Jan

Fingerprint

Organoids
Retroviridae
Cell culture
Gene expression
Small Intestine
Stomach
Colon
Animals
Epithelium
Animal Models
Genes
Gene Expression

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Koo, B. K., Stange, D. E., Sato, T., Karthaus, W., Farin, H. F., Huch, M., ... Clevers, H. (2012). Controlled gene expression in primary Lgr5 organoid cultures. Nature Methods, 9(1), 81-83. https://doi.org/10.1038/nmeth.1802

Controlled gene expression in primary Lgr5 organoid cultures. / Koo, Bon Kyoung; Stange, Daniel E.; Sato, Toshiro; Karthaus, Wouter; Farin, Henner F.; Huch, Meritxell; Van Es, Johan H.; Clevers, Hans.

In: Nature Methods, Vol. 9, No. 1, 01.2012, p. 81-83.

Research output: Contribution to journalArticle

Koo, BK, Stange, DE, Sato, T, Karthaus, W, Farin, HF, Huch, M, Van Es, JH & Clevers, H 2012, 'Controlled gene expression in primary Lgr5 organoid cultures', Nature Methods, vol. 9, no. 1, pp. 81-83. https://doi.org/10.1038/nmeth.1802
Koo BK, Stange DE, Sato T, Karthaus W, Farin HF, Huch M et al. Controlled gene expression in primary Lgr5 organoid cultures. Nature Methods. 2012 Jan;9(1):81-83. https://doi.org/10.1038/nmeth.1802
Koo, Bon Kyoung ; Stange, Daniel E. ; Sato, Toshiro ; Karthaus, Wouter ; Farin, Henner F. ; Huch, Meritxell ; Van Es, Johan H. ; Clevers, Hans. / Controlled gene expression in primary Lgr5 organoid cultures. In: Nature Methods. 2012 ; Vol. 9, No. 1. pp. 81-83.
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