Abstract
The CNS contains many diverse neuronal subtypes, and most neurological diseases target specific subtypes. However, the mechanism of neuronal subtype specificity of disease phenotypes remains elusive. Although in vitro disease models employing human pluripotent stem cells (PSCs) have great potential to clarify the association of neuronal subtypes with disease, it is currently difficult to compare various PSC-derived subtypes. This is due to the limited number of subtypes whose induction is established, and different cultivation protocols for each subtype. Here, we report a culture system to control the regional identity of PSC-derived neurons along the anteroposterior (A-P) and dorsoventral (D-V) axes. This system was successfully used to obtain various neuronal subtypes based on the same protocol. Furthermore, we reproduced subtype-specific phenotypes of amyotrophic lateral sclerosis (ALS) and Alzheimer's disease (AD) by comparing the obtained subtypes. Therefore, our culture system provides new opportunities for modeling neurological diseases with PSCs.
| Original language | English |
|---|---|
| Pages (from-to) | 1010-1022 |
| Number of pages | 13 |
| Journal | Stem Cell Reports |
| Volume | 5 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2015 Dec 8 |
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ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Developmental Biology
- Genetics
Cite this
Controlling the Regional Identity of hPSC-Derived Neurons to Uncover Neuronal Subtype Specificity of Neurological Disease Phenotypes. / Imaizumi, Kent; Sone, Takefumi; Ibata, Keiji; Fujimori, Koki; Yuzaki, Michisuke; Akamatsu, Wado; Okano, Hideyuki.
In: Stem Cell Reports, Vol. 5, No. 6, 08.12.2015, p. 1010-1022.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Controlling the Regional Identity of hPSC-Derived Neurons to Uncover Neuronal Subtype Specificity of Neurological Disease Phenotypes
AU - Imaizumi, Kent
AU - Sone, Takefumi
AU - Ibata, Keiji
AU - Fujimori, Koki
AU - Yuzaki, Michisuke
AU - Akamatsu, Wado
AU - Okano, Hideyuki
PY - 2015/12/8
Y1 - 2015/12/8
N2 - The CNS contains many diverse neuronal subtypes, and most neurological diseases target specific subtypes. However, the mechanism of neuronal subtype specificity of disease phenotypes remains elusive. Although in vitro disease models employing human pluripotent stem cells (PSCs) have great potential to clarify the association of neuronal subtypes with disease, it is currently difficult to compare various PSC-derived subtypes. This is due to the limited number of subtypes whose induction is established, and different cultivation protocols for each subtype. Here, we report a culture system to control the regional identity of PSC-derived neurons along the anteroposterior (A-P) and dorsoventral (D-V) axes. This system was successfully used to obtain various neuronal subtypes based on the same protocol. Furthermore, we reproduced subtype-specific phenotypes of amyotrophic lateral sclerosis (ALS) and Alzheimer's disease (AD) by comparing the obtained subtypes. Therefore, our culture system provides new opportunities for modeling neurological diseases with PSCs.
AB - The CNS contains many diverse neuronal subtypes, and most neurological diseases target specific subtypes. However, the mechanism of neuronal subtype specificity of disease phenotypes remains elusive. Although in vitro disease models employing human pluripotent stem cells (PSCs) have great potential to clarify the association of neuronal subtypes with disease, it is currently difficult to compare various PSC-derived subtypes. This is due to the limited number of subtypes whose induction is established, and different cultivation protocols for each subtype. Here, we report a culture system to control the regional identity of PSC-derived neurons along the anteroposterior (A-P) and dorsoventral (D-V) axes. This system was successfully used to obtain various neuronal subtypes based on the same protocol. Furthermore, we reproduced subtype-specific phenotypes of amyotrophic lateral sclerosis (ALS) and Alzheimer's disease (AD) by comparing the obtained subtypes. Therefore, our culture system provides new opportunities for modeling neurological diseases with PSCs.
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UR - http://www.scopus.com/inward/citedby.url?scp=84949537366&partnerID=8YFLogxK
U2 - 10.1016/j.stemcr.2015.10.005
DO - 10.1016/j.stemcr.2015.10.005
M3 - Article
VL - 5
SP - 1010
EP - 1022
JO - Stem Cell Reports
JF - Stem Cell Reports
SN - 2213-6711
IS - 6
ER -