Conversion of β-human atrial natriuretic polypeptide into α-human atrial natriuretic polypeptide in human plasma in vitro

Hiroshi Itoh, Kazuwa Nakao, Shozo Shiono, Masashi Mukoyama, Narito Morii, Akira Sugawara, Takayuki Yamada, Yoshihiko Saito, Hiroshi Arai, Yoshikazu Kambayashi, Ken Inouye, Hiroo Imura

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21 Citations (Scopus)

Abstract

Using synthetic β-human atrial natriuretic polypeptide (β-hANP), an antiparallel dimer of α-hANP, and radioimmunoassay (RIA) for α-ANP which also detects β-hANP, we investigated the disappearance profile and the change in the molecular form of exogenously added β-hANP in human plasma in vitro, compared with those of α-hANP. The ANP-like immunoreactivity (ANP-LI) level in β-hANP-added human plasma exhibited slower disappearance than that in α-hANP-added plasma during the incubation at 37°C. High performance-gel permeation chromatography and reverse phase-high performance liquid chromatography coupled with RIA revealed that β-hANP (6K) was converted into a smaller peptide with an approximate molecular weight of 3K corresponding to α-hANP during the incubation. Amino acid analysis and amino-terminal sequencing confirmed that the converted peptide from β-hANP in human plasma is authentic α-hANP. The demonstrated conversion of β-hANP into α-hANP in human plasma could be relevant to the in vivo natriuretic and diuretic actions with slower onset and longer duration of this unique peptide.

Original languageEnglish
Pages (from-to)560-569
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume143
Issue number2
DOIs
Publication statusPublished - 1987 Mar 13
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Itoh, H., Nakao, K., Shiono, S., Mukoyama, M., Morii, N., Sugawara, A., Yamada, T., Saito, Y., Arai, H., Kambayashi, Y., Inouye, K., & Imura, H. (1987). Conversion of β-human atrial natriuretic polypeptide into α-human atrial natriuretic polypeptide in human plasma in vitro. Biochemical and Biophysical Research Communications, 143(2), 560-569. https://doi.org/10.1016/0006-291X(87)91390-8