Conversion of a chaperonin GroEL-independent protein into an obligate substrate

Takuya Ishimoto, Kei Fujiwara, Tatsuya Niwa, Hideki Taguchi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Chaperones assist protein folding by preventing unproductive protein aggregation in the cell. In Escherichia coli, chaperonin GroEL/GroES (GroE) is the only indispensable chaperone and is absolutely required for the de novo folding of at least ∼60 proteins. We previously found that several orthologs of the obligate GroE substrates in Ureaplasma urealyticum, which lacks the groE gene in the genome, are E. coli GroE-independent folders, despite their significant sequence identities. Here, we investigated the key features that define the GroE dependence. Chimera or random mutagenesis analyses revealed that independent multiple point mutations, and even single mutations, were sufficient to confer GroE dependence on the Ureaplasma MetK. Strikingly, the GroE dependence was well correlated with the propensity to form protein aggregates during folding. The results reveal the delicate balance between GroE dependence and independence. The function of GroE to buffering the aggregation-prone mutations plays a role in maintaining higher genetic diversity of proteins.

Original languageEnglish
Pages (from-to)32073-32080
Number of pages8
JournalJournal of Biological Chemistry
Volume289
Issue number46
DOIs
Publication statusPublished - 2014 Nov 14

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Chaperonins
Chaperonin 60
Escherichia coli
Substrates
Agglomeration
Genes
Ureaplasma
Ureaplasma urealyticum
Protein folding
Cell Aggregation
Mutagenesis
Mutation
Proteins
Protein Folding
Point Mutation
Genome

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Conversion of a chaperonin GroEL-independent protein into an obligate substrate. / Ishimoto, Takuya; Fujiwara, Kei; Niwa, Tatsuya; Taguchi, Hideki.

In: Journal of Biological Chemistry, Vol. 289, No. 46, 14.11.2014, p. 32073-32080.

Research output: Contribution to journalArticle

Ishimoto, Takuya ; Fujiwara, Kei ; Niwa, Tatsuya ; Taguchi, Hideki. / Conversion of a chaperonin GroEL-independent protein into an obligate substrate. In: Journal of Biological Chemistry. 2014 ; Vol. 289, No. 46. pp. 32073-32080.
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