TY - CHAP
T1 - Conversion of primordial germ cells to pluripotent stem cells
T2 - Methods for cell tracking and culture conditions
AU - Nagamatsu, Go
AU - Suda, Toshio
PY - 2013/9/4
Y1 - 2013/9/4
N2 - Primordial germ cells (PGCs) are unipotent cells committed to germ lineage: PGCs can only differentiate into gametes in vivo. However, upon fertilization, germ cells acquire the capacity to differentiate into all cell types in the body, including germ cells. Therefore, germ cells are thought to have the potential for pluripotency. PGCs can convert to pluripotent stem cells in vitro when cultured under specific conditions that include bFGF, LIF, and the membrane-bound form of SCF (mSCF). Here, the culture conditions which efficiently convert PGCs to pluripotent embryonic germ (EG) cells are described, as well as methods used for identifying pluripotent candidate cells during culture.
AB - Primordial germ cells (PGCs) are unipotent cells committed to germ lineage: PGCs can only differentiate into gametes in vivo. However, upon fertilization, germ cells acquire the capacity to differentiate into all cell types in the body, including germ cells. Therefore, germ cells are thought to have the potential for pluripotency. PGCs can convert to pluripotent stem cells in vitro when cultured under specific conditions that include bFGF, LIF, and the membrane-bound form of SCF (mSCF). Here, the culture conditions which efficiently convert PGCs to pluripotent embryonic germ (EG) cells are described, as well as methods used for identifying pluripotent candidate cells during culture.
KW - Acquisition of pluripotency
KW - Primordial germ cell
KW - Purification
KW - Tracking
UR - http://www.scopus.com/inward/record.url?scp=84883236998&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883236998&partnerID=8YFLogxK
U2 - 10.1007/7651-2013-24
DO - 10.1007/7651-2013-24
M3 - Chapter
C2 - 23640254
AN - SCOPUS:84883236998
SN - 9781627035583
T3 - Methods in Molecular Biology
SP - 49
EP - 56
BT - Imaging and Tracking Stem Cells
ER -