Conversion of primordial germ cells to pluripotent stem cells: Methods for cell tracking and culture conditions

Go Nagamatsu, Toshio Suda

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Primordial germ cells (PGCs) are unipotent cells committed to germ lineage: PGCs can only differentiate into gametes in vivo. However, upon fertilization, germ cells acquire the capacity to differentiate into all cell types in the body, including germ cells. Therefore, germ cells are thought to have the potential for pluripotency. PGCs can convert to pluripotent stem cells in vitro when cultured under specific conditions that include bFGF, LIF, and the membrane-bound form of SCF (mSCF). Here, the culture conditions which efficiently convert PGCs to pluripotent embryonic germ (EG) cells are described, as well as methods used for identifying pluripotent candidate cells during culture.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages49-56
Number of pages8
Volume1052
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1052
ISSN (Print)10643745

Fingerprint

Cell Tracking
Pluripotent Stem Cells
Germ Cells
Cell Culture Techniques
Fertilization
Membranes

Keywords

  • Acquisition of pluripotency
  • Primordial germ cell
  • Purification
  • Tracking

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Nagamatsu, G., & Suda, T. (2013). Conversion of primordial germ cells to pluripotent stem cells: Methods for cell tracking and culture conditions. In Methods in Molecular Biology (Vol. 1052, pp. 49-56). (Methods in Molecular Biology; Vol. 1052). https://doi.org/10.1007/7651-2013-24

Conversion of primordial germ cells to pluripotent stem cells : Methods for cell tracking and culture conditions. / Nagamatsu, Go; Suda, Toshio.

Methods in Molecular Biology. Vol. 1052 2013. p. 49-56 (Methods in Molecular Biology; Vol. 1052).

Research output: Chapter in Book/Report/Conference proceedingChapter

Nagamatsu, G & Suda, T 2013, Conversion of primordial germ cells to pluripotent stem cells: Methods for cell tracking and culture conditions. in Methods in Molecular Biology. vol. 1052, Methods in Molecular Biology, vol. 1052, pp. 49-56. https://doi.org/10.1007/7651-2013-24
Nagamatsu G, Suda T. Conversion of primordial germ cells to pluripotent stem cells: Methods for cell tracking and culture conditions. In Methods in Molecular Biology. Vol. 1052. 2013. p. 49-56. (Methods in Molecular Biology). https://doi.org/10.1007/7651-2013-24
Nagamatsu, Go ; Suda, Toshio. / Conversion of primordial germ cells to pluripotent stem cells : Methods for cell tracking and culture conditions. Methods in Molecular Biology. Vol. 1052 2013. pp. 49-56 (Methods in Molecular Biology).
@inbook{33cf922d72a84e3c85d5815cd1f28fdd,
title = "Conversion of primordial germ cells to pluripotent stem cells: Methods for cell tracking and culture conditions",
abstract = "Primordial germ cells (PGCs) are unipotent cells committed to germ lineage: PGCs can only differentiate into gametes in vivo. However, upon fertilization, germ cells acquire the capacity to differentiate into all cell types in the body, including germ cells. Therefore, germ cells are thought to have the potential for pluripotency. PGCs can convert to pluripotent stem cells in vitro when cultured under specific conditions that include bFGF, LIF, and the membrane-bound form of SCF (mSCF). Here, the culture conditions which efficiently convert PGCs to pluripotent embryonic germ (EG) cells are described, as well as methods used for identifying pluripotent candidate cells during culture.",
keywords = "Acquisition of pluripotency, Primordial germ cell, Purification, Tracking",
author = "Go Nagamatsu and Toshio Suda",
year = "2013",
doi = "10.1007/7651-2013-24",
language = "English",
isbn = "9781627035583",
volume = "1052",
series = "Methods in Molecular Biology",
pages = "49--56",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Conversion of primordial germ cells to pluripotent stem cells

T2 - Methods for cell tracking and culture conditions

AU - Nagamatsu, Go

AU - Suda, Toshio

PY - 2013

Y1 - 2013

N2 - Primordial germ cells (PGCs) are unipotent cells committed to germ lineage: PGCs can only differentiate into gametes in vivo. However, upon fertilization, germ cells acquire the capacity to differentiate into all cell types in the body, including germ cells. Therefore, germ cells are thought to have the potential for pluripotency. PGCs can convert to pluripotent stem cells in vitro when cultured under specific conditions that include bFGF, LIF, and the membrane-bound form of SCF (mSCF). Here, the culture conditions which efficiently convert PGCs to pluripotent embryonic germ (EG) cells are described, as well as methods used for identifying pluripotent candidate cells during culture.

AB - Primordial germ cells (PGCs) are unipotent cells committed to germ lineage: PGCs can only differentiate into gametes in vivo. However, upon fertilization, germ cells acquire the capacity to differentiate into all cell types in the body, including germ cells. Therefore, germ cells are thought to have the potential for pluripotency. PGCs can convert to pluripotent stem cells in vitro when cultured under specific conditions that include bFGF, LIF, and the membrane-bound form of SCF (mSCF). Here, the culture conditions which efficiently convert PGCs to pluripotent embryonic germ (EG) cells are described, as well as methods used for identifying pluripotent candidate cells during culture.

KW - Acquisition of pluripotency

KW - Primordial germ cell

KW - Purification

KW - Tracking

UR - http://www.scopus.com/inward/record.url?scp=84883236998&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84883236998&partnerID=8YFLogxK

U2 - 10.1007/7651-2013-24

DO - 10.1007/7651-2013-24

M3 - Chapter

C2 - 23640254

AN - SCOPUS:84883236998

SN - 9781627035583

VL - 1052

T3 - Methods in Molecular Biology

SP - 49

EP - 56

BT - Methods in Molecular Biology

ER -