Abstract
The melibiose utilization system of Escherichia coli W3133, a derivative of K12, is nonfunctional between 37 and 42 °C. The reason for this temperature sensitivity was thought to be that the melibiose transporter (MelB) of W3133 cells was temperature-sensitive. A mutant W3133-2 has been isolated as a temperature-resistant strain that can utilize melibiose between 37 and 42 °C. However, we found that the melibiose transporter of the W3133- 2 was still temperature-sensitive. Half-life activities of the melibiose transporter at 37 °C (or 40 °C) in both E. coli W3133 and W3133-2 were exactly the same. Furthermore, we found that the nucleotide sequence of coding region of the melB structural gene (the second gene of the melibiose operon) of W3133-2 was exactly the same as that of W3133. Activity of α- galactosidase (product of the first gene, melA, of the melibiose operon) of W3133 cells grown at 40 °C was very low, although that of W3133-2 cells grown at 40 °C was high. These observations suggested that expression of the melibiose operon in W3133 is also temperature-sensitive. In fact, we found that the expression in W3133 cells was temperature-sensitive, while that in W3133-2 cells was temperature-resistant, by analyzing mRNA levels using the Northern blot method. Furthermore, we identified mutations in the promoter region of the melibiose operon of W3133-2 that resulted in the elongation of an 18 nucleotide inverted repeat sequence to a 28-nucleotide repeat sequence present immediately upstream of the -35 region. This may stabilize a possible stem structure due to the inverted repeat at 37-72 °C.
| Original language | English |
|---|---|
| Pages (from-to) | 16860-16864 |
| Number of pages | 5 |
| Journal | Journal of Biological Chemistry |
| Volume | 273 |
| Issue number | 27 |
| DOIs | |
| Publication status | Published - 1998 Jul 3 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
Cite this
Conversion of temperature-sensitive to -resistant gene expression due to mutations in the promoter region of the melibiose operon in Escherichia coli. / Tamai, Eiji; Shimamoto, Tadashi; Tsuda, Masaaki; Mizushima, Tohru; Tsuchiya, Tomofusa.
In: Journal of Biological Chemistry, Vol. 273, No. 27, 03.07.1998, p. 16860-16864.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Conversion of temperature-sensitive to -resistant gene expression due to mutations in the promoter region of the melibiose operon in Escherichia coli
AU - Tamai, Eiji
AU - Shimamoto, Tadashi
AU - Tsuda, Masaaki
AU - Mizushima, Tohru
AU - Tsuchiya, Tomofusa
PY - 1998/7/3
Y1 - 1998/7/3
N2 - The melibiose utilization system of Escherichia coli W3133, a derivative of K12, is nonfunctional between 37 and 42 °C. The reason for this temperature sensitivity was thought to be that the melibiose transporter (MelB) of W3133 cells was temperature-sensitive. A mutant W3133-2 has been isolated as a temperature-resistant strain that can utilize melibiose between 37 and 42 °C. However, we found that the melibiose transporter of the W3133- 2 was still temperature-sensitive. Half-life activities of the melibiose transporter at 37 °C (or 40 °C) in both E. coli W3133 and W3133-2 were exactly the same. Furthermore, we found that the nucleotide sequence of coding region of the melB structural gene (the second gene of the melibiose operon) of W3133-2 was exactly the same as that of W3133. Activity of α- galactosidase (product of the first gene, melA, of the melibiose operon) of W3133 cells grown at 40 °C was very low, although that of W3133-2 cells grown at 40 °C was high. These observations suggested that expression of the melibiose operon in W3133 is also temperature-sensitive. In fact, we found that the expression in W3133 cells was temperature-sensitive, while that in W3133-2 cells was temperature-resistant, by analyzing mRNA levels using the Northern blot method. Furthermore, we identified mutations in the promoter region of the melibiose operon of W3133-2 that resulted in the elongation of an 18 nucleotide inverted repeat sequence to a 28-nucleotide repeat sequence present immediately upstream of the -35 region. This may stabilize a possible stem structure due to the inverted repeat at 37-72 °C.
AB - The melibiose utilization system of Escherichia coli W3133, a derivative of K12, is nonfunctional between 37 and 42 °C. The reason for this temperature sensitivity was thought to be that the melibiose transporter (MelB) of W3133 cells was temperature-sensitive. A mutant W3133-2 has been isolated as a temperature-resistant strain that can utilize melibiose between 37 and 42 °C. However, we found that the melibiose transporter of the W3133- 2 was still temperature-sensitive. Half-life activities of the melibiose transporter at 37 °C (or 40 °C) in both E. coli W3133 and W3133-2 were exactly the same. Furthermore, we found that the nucleotide sequence of coding region of the melB structural gene (the second gene of the melibiose operon) of W3133-2 was exactly the same as that of W3133. Activity of α- galactosidase (product of the first gene, melA, of the melibiose operon) of W3133 cells grown at 40 °C was very low, although that of W3133-2 cells grown at 40 °C was high. These observations suggested that expression of the melibiose operon in W3133 is also temperature-sensitive. In fact, we found that the expression in W3133 cells was temperature-sensitive, while that in W3133-2 cells was temperature-resistant, by analyzing mRNA levels using the Northern blot method. Furthermore, we identified mutations in the promoter region of the melibiose operon of W3133-2 that resulted in the elongation of an 18 nucleotide inverted repeat sequence to a 28-nucleotide repeat sequence present immediately upstream of the -35 region. This may stabilize a possible stem structure due to the inverted repeat at 37-72 °C.
UR - http://www.scopus.com/inward/record.url?scp=0032479166&partnerID=8YFLogxK
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U2 - 10.1074/jbc.273.27.16860
DO - 10.1074/jbc.273.27.16860
M3 - Article
C2 - 9642246
AN - SCOPUS:0032479166
VL - 273
SP - 16860
EP - 16864
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 27
ER -