Conversion of temperature-sensitive to -resistant gene expression due to mutations in the promoter region of the melibiose operon in Escherichia coli

Eiji Tamai, Tadashi Shimamoto, Masaaki Tsuda, Tohru Mizushima, Tomofusa Tsuchiya

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The melibiose utilization system of Escherichia coli W3133, a derivative of K12, is nonfunctional between 37 and 42 °C. The reason for this temperature sensitivity was thought to be that the melibiose transporter (MelB) of W3133 cells was temperature-sensitive. A mutant W3133-2 has been isolated as a temperature-resistant strain that can utilize melibiose between 37 and 42 °C. However, we found that the melibiose transporter of the W3133- 2 was still temperature-sensitive. Half-life activities of the melibiose transporter at 37 °C (or 40 °C) in both E. coli W3133 and W3133-2 were exactly the same. Furthermore, we found that the nucleotide sequence of coding region of the melB structural gene (the second gene of the melibiose operon) of W3133-2 was exactly the same as that of W3133. Activity of α- galactosidase (product of the first gene, melA, of the melibiose operon) of W3133 cells grown at 40 °C was very low, although that of W3133-2 cells grown at 40 °C was high. These observations suggested that expression of the melibiose operon in W3133 is also temperature-sensitive. In fact, we found that the expression in W3133 cells was temperature-sensitive, while that in W3133-2 cells was temperature-resistant, by analyzing mRNA levels using the Northern blot method. Furthermore, we identified mutations in the promoter region of the melibiose operon of W3133-2 that resulted in the elongation of an 18 nucleotide inverted repeat sequence to a 28-nucleotide repeat sequence present immediately upstream of the -35 region. This may stabilize a possible stem structure due to the inverted repeat at 37-72 °C.

Original languageEnglish
Pages (from-to)16860-16864
Number of pages5
JournalJournal of Biological Chemistry
Volume273
Issue number27
DOIs
Publication statusPublished - 1998 Jul 3
Externally publishedYes

Fingerprint

Melibiose
Operon
Genetic Promoter Regions
Gene expression
Escherichia coli
Gene Expression
Mutation
Temperature
Nucleotides
Genes
Cells
Galactosidases
Inverted Repeat Sequences
Northern Blotting
Half-Life
Elongation
Derivatives
Messenger RNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Conversion of temperature-sensitive to -resistant gene expression due to mutations in the promoter region of the melibiose operon in Escherichia coli. / Tamai, Eiji; Shimamoto, Tadashi; Tsuda, Masaaki; Mizushima, Tohru; Tsuchiya, Tomofusa.

In: Journal of Biological Chemistry, Vol. 273, No. 27, 03.07.1998, p. 16860-16864.

Research output: Contribution to journalArticle

Tamai, Eiji ; Shimamoto, Tadashi ; Tsuda, Masaaki ; Mizushima, Tohru ; Tsuchiya, Tomofusa. / Conversion of temperature-sensitive to -resistant gene expression due to mutations in the promoter region of the melibiose operon in Escherichia coli. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 27. pp. 16860-16864.
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