TY - JOUR
T1 - Coordinated expression of HuD and GAP-43 in hippocampal dentate granule cells during developmental and adult plasticity
AU - Bolognani, Federico
AU - Tanner, Daniel C.
AU - Nixon, Sayuri
AU - Okano, Hirotaka J.
AU - Okano, Hideyuki
AU - Perrone-Bizzozero, Nora I.
N1 - Funding Information:
Acknowledgments We dedicate this work to Dr. Naren Banik, in recognition to his outstanding achievements in multiple sclerosis and spinal cord injury research. This work was supported by NIH (NS30255) to NPB.
PY - 2007/12
Y1 - 2007/12
N2 - Previous work from our laboratory demonstrated that the RNA-binding protein HuD binds to and stabilizes the GAP-43 mRNA. In this study, we characterized the expression of HuD and GAP-43 mRNA in the hippocampus during two forms of neuronal plasticity. During post-natal development, maximal expression of both molecules was found at P5 and their levels steadily decreased thereafter. At P5, HuD was also present in the subventricular zone, where it co-localized with doublecortin. In the adult hippocampus, the basal levels of HuD and GAP-43 were lower than during development but were significantly increased in the dentate gyrus after seizures. The function of HuD in GAP-43 gene expression was confirmed using HuD-KO mice, in which the GAP-43 mRNA was significantly less stable than in wild type mice. Altogether, these results demonstrate that HuD plays a role in the post-transcriptional control of GAP-43 mRNA in dentate granule cells during developmental and adult plasticity.
AB - Previous work from our laboratory demonstrated that the RNA-binding protein HuD binds to and stabilizes the GAP-43 mRNA. In this study, we characterized the expression of HuD and GAP-43 mRNA in the hippocampus during two forms of neuronal plasticity. During post-natal development, maximal expression of both molecules was found at P5 and their levels steadily decreased thereafter. At P5, HuD was also present in the subventricular zone, where it co-localized with doublecortin. In the adult hippocampus, the basal levels of HuD and GAP-43 were lower than during development but were significantly increased in the dentate gyrus after seizures. The function of HuD in GAP-43 gene expression was confirmed using HuD-KO mice, in which the GAP-43 mRNA was significantly less stable than in wild type mice. Altogether, these results demonstrate that HuD plays a role in the post-transcriptional control of GAP-43 mRNA in dentate granule cells during developmental and adult plasticity.
KW - Dentate granule cells
KW - Development
KW - Epilepsy
KW - Post-transcriptional regulation
KW - RNA-binding protein
KW - mRNA stability
UR - http://www.scopus.com/inward/record.url?scp=35848966583&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=35848966583&partnerID=8YFLogxK
U2 - 10.1007/s11064-007-9388-8
DO - 10.1007/s11064-007-9388-8
M3 - Article
C2 - 17577668
AN - SCOPUS:35848966583
SN - 0364-3190
VL - 32
SP - 2142
EP - 2151
JO - Neurochemical Research
JF - Neurochemical Research
IS - 12
ER -