Coordinated expression of HuD and GAP-43 in hippocampal dentate granule cells during developmental and adult plasticity

Federico Bolognani, Daniel C. Tanner, Sayuri Nixon, Hirotaka J. Okano, Hideyuki Okano, Nora I. Perrone-Bizzozero

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Previous work from our laboratory demonstrated that the RNA-binding protein HuD binds to and stabilizes the GAP-43 mRNA. In this study, we characterized the expression of HuD and GAP-43 mRNA in the hippocampus during two forms of neuronal plasticity. During post-natal development, maximal expression of both molecules was found at P5 and their levels steadily decreased thereafter. At P5, HuD was also present in the subventricular zone, where it co-localized with doublecortin. In the adult hippocampus, the basal levels of HuD and GAP-43 were lower than during development but were significantly increased in the dentate gyrus after seizures. The function of HuD in GAP-43 gene expression was confirmed using HuD-KO mice, in which the GAP-43 mRNA was significantly less stable than in wild type mice. Altogether, these results demonstrate that HuD plays a role in the post-transcriptional control of GAP-43 mRNA in dentate granule cells during developmental and adult plasticity.

Original languageEnglish
Pages (from-to)2142-2151
Number of pages10
JournalNeurochemical Research
Volume32
Issue number12
DOIs
Publication statusPublished - 2007 Dec

Fingerprint

GAP-43 Protein
Plasticity
Messenger RNA
Hippocampus
Neuronal Plasticity
RNA-Binding Proteins
Lateral Ventricles
Dentate Gyrus
Gene expression
Seizures
Gene Expression
Molecules

Keywords

  • Dentate granule cells
  • Development
  • Epilepsy
  • mRNA stability
  • Post-transcriptional regulation
  • RNA-binding protein

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Coordinated expression of HuD and GAP-43 in hippocampal dentate granule cells during developmental and adult plasticity. / Bolognani, Federico; Tanner, Daniel C.; Nixon, Sayuri; Okano, Hirotaka J.; Okano, Hideyuki; Perrone-Bizzozero, Nora I.

In: Neurochemical Research, Vol. 32, No. 12, 12.2007, p. 2142-2151.

Research output: Contribution to journalArticle

Bolognani, Federico ; Tanner, Daniel C. ; Nixon, Sayuri ; Okano, Hirotaka J. ; Okano, Hideyuki ; Perrone-Bizzozero, Nora I. / Coordinated expression of HuD and GAP-43 in hippocampal dentate granule cells during developmental and adult plasticity. In: Neurochemical Research. 2007 ; Vol. 32, No. 12. pp. 2142-2151.
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