Corneal and conjunctival fibroblasts are major sources of eosinophil-recruiting chemokines

Kazumi Fukagawa, Naoko Okada, Hiroshi Fujishima, Toshiharu Nakajima, Yoji Takano, Mari Tanaka, Murat Dogru, Yoshiyuki Satake, Kazuo Tsubota, Hirohisa Saito

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background: Differential expression of chemokine genes were investigated in various types of ocular surface cells. Methods: Primary cultures of human corneal epithelial cells (n = 3), corneal fibroblasts (n = 2), conjunctival epithelial cells (n = 2) and conjunctival fibroblasts (n = 2) were established and incubated with or without interleukin (IL)-4 (30 ng/ml) and tumor necrosis factor (TNF)-α (30 ng/ml) for 24 hours. Gene transcription levels of 33 chemokines and production of 4 chemokines were analyzed. Results: After stimulation, chemokine expression increased for 18 of 33 coded chemokine gene transcripts. In stimulated conjunctival and corneal cells, CC chemokine genes increased in fibroblasts (expression of 6 out of 8 genes), while CXC chemokine genes increased in both epithelial cells (expression of 4 out of 9 genes in conjunctival epithelial cells and 7 out of 9 genes in corneal epithelial cells) and in fibroblasts (expression of 8 out of 9 genes in conjunctival and corneal fibroblasts). Except for MCP-1, gene transcription levels for most CC chemokines were inducible and, except for IP-10 and I-TAC, most CXC chemokines were constitutively expressed. Corneal epithelial cell and fibroblast production patterns for eotaxin-1, MCP-1 and IP-10 were comparable to the mRNA expression pattern. Conclusions: Corneal and conjunctival fibroblasts exhibited marked increases in the expression of chemokines upon stimulation with TNF-α and IL-4, suggesting that fibroblasts may be one of the primary sources of chemokines in allergic conjunctival diseases. Therefore, regulation of chemokine production from these cells may be an effective strategy for treating such diseases.

Original languageEnglish
Pages (from-to)499-508
Number of pages10
JournalAllergology International
Volume58
Issue number4
DOIs
Publication statusPublished - 2009

Fingerprint

Chemokines
Eosinophils
Fibroblasts
Epithelial Cells
Genes
CC Chemokines
Interleukin-4
Chemokine CXCL11
Conjunctival Diseases
Chemokine CCL11
Tumor Necrosis Factor-alpha
CXC Chemokines
Gene Expression
Messenger RNA

Keywords

  • Chemokine
  • Conjunctiva
  • Epithelial cells
  • Fibroblast
  • Gene expression

ASJC Scopus subject areas

  • Immunology and Allergy

Cite this

Fukagawa, K., Okada, N., Fujishima, H., Nakajima, T., Takano, Y., Tanaka, M., ... Saito, H. (2009). Corneal and conjunctival fibroblasts are major sources of eosinophil-recruiting chemokines. Allergology International, 58(4), 499-508. https://doi.org/10.2332/allergolint.09-OA-0092

Corneal and conjunctival fibroblasts are major sources of eosinophil-recruiting chemokines. / Fukagawa, Kazumi; Okada, Naoko; Fujishima, Hiroshi; Nakajima, Toshiharu; Takano, Yoji; Tanaka, Mari; Dogru, Murat; Satake, Yoshiyuki; Tsubota, Kazuo; Saito, Hirohisa.

In: Allergology International, Vol. 58, No. 4, 2009, p. 499-508.

Research output: Contribution to journalArticle

Fukagawa, K, Okada, N, Fujishima, H, Nakajima, T, Takano, Y, Tanaka, M, Dogru, M, Satake, Y, Tsubota, K & Saito, H 2009, 'Corneal and conjunctival fibroblasts are major sources of eosinophil-recruiting chemokines', Allergology International, vol. 58, no. 4, pp. 499-508. https://doi.org/10.2332/allergolint.09-OA-0092
Fukagawa, Kazumi ; Okada, Naoko ; Fujishima, Hiroshi ; Nakajima, Toshiharu ; Takano, Yoji ; Tanaka, Mari ; Dogru, Murat ; Satake, Yoshiyuki ; Tsubota, Kazuo ; Saito, Hirohisa. / Corneal and conjunctival fibroblasts are major sources of eosinophil-recruiting chemokines. In: Allergology International. 2009 ; Vol. 58, No. 4. pp. 499-508.
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AU - Fukagawa, Kazumi

AU - Okada, Naoko

AU - Fujishima, Hiroshi

AU - Nakajima, Toshiharu

AU - Takano, Yoji

AU - Tanaka, Mari

AU - Dogru, Murat

AU - Satake, Yoshiyuki

AU - Tsubota, Kazuo

AU - Saito, Hirohisa

PY - 2009

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N2 - Background: Differential expression of chemokine genes were investigated in various types of ocular surface cells. Methods: Primary cultures of human corneal epithelial cells (n = 3), corneal fibroblasts (n = 2), conjunctival epithelial cells (n = 2) and conjunctival fibroblasts (n = 2) were established and incubated with or without interleukin (IL)-4 (30 ng/ml) and tumor necrosis factor (TNF)-α (30 ng/ml) for 24 hours. Gene transcription levels of 33 chemokines and production of 4 chemokines were analyzed. Results: After stimulation, chemokine expression increased for 18 of 33 coded chemokine gene transcripts. In stimulated conjunctival and corneal cells, CC chemokine genes increased in fibroblasts (expression of 6 out of 8 genes), while CXC chemokine genes increased in both epithelial cells (expression of 4 out of 9 genes in conjunctival epithelial cells and 7 out of 9 genes in corneal epithelial cells) and in fibroblasts (expression of 8 out of 9 genes in conjunctival and corneal fibroblasts). Except for MCP-1, gene transcription levels for most CC chemokines were inducible and, except for IP-10 and I-TAC, most CXC chemokines were constitutively expressed. Corneal epithelial cell and fibroblast production patterns for eotaxin-1, MCP-1 and IP-10 were comparable to the mRNA expression pattern. Conclusions: Corneal and conjunctival fibroblasts exhibited marked increases in the expression of chemokines upon stimulation with TNF-α and IL-4, suggesting that fibroblasts may be one of the primary sources of chemokines in allergic conjunctival diseases. Therefore, regulation of chemokine production from these cells may be an effective strategy for treating such diseases.

AB - Background: Differential expression of chemokine genes were investigated in various types of ocular surface cells. Methods: Primary cultures of human corneal epithelial cells (n = 3), corneal fibroblasts (n = 2), conjunctival epithelial cells (n = 2) and conjunctival fibroblasts (n = 2) were established and incubated with or without interleukin (IL)-4 (30 ng/ml) and tumor necrosis factor (TNF)-α (30 ng/ml) for 24 hours. Gene transcription levels of 33 chemokines and production of 4 chemokines were analyzed. Results: After stimulation, chemokine expression increased for 18 of 33 coded chemokine gene transcripts. In stimulated conjunctival and corneal cells, CC chemokine genes increased in fibroblasts (expression of 6 out of 8 genes), while CXC chemokine genes increased in both epithelial cells (expression of 4 out of 9 genes in conjunctival epithelial cells and 7 out of 9 genes in corneal epithelial cells) and in fibroblasts (expression of 8 out of 9 genes in conjunctival and corneal fibroblasts). Except for MCP-1, gene transcription levels for most CC chemokines were inducible and, except for IP-10 and I-TAC, most CXC chemokines were constitutively expressed. Corneal epithelial cell and fibroblast production patterns for eotaxin-1, MCP-1 and IP-10 were comparable to the mRNA expression pattern. Conclusions: Corneal and conjunctival fibroblasts exhibited marked increases in the expression of chemokines upon stimulation with TNF-α and IL-4, suggesting that fibroblasts may be one of the primary sources of chemokines in allergic conjunctival diseases. Therefore, regulation of chemokine production from these cells may be an effective strategy for treating such diseases.

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KW - Conjunctiva

KW - Epithelial cells

KW - Fibroblast

KW - Gene expression

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