Corneal and retinal effects of ultraviolet-B exposure in a soft contact lens mouse model

Osama M A Ibrahim, Takashi Kojima, Tais Hitomi Wakamatsu, Murat Dogru, Yukihiro Matsumoto, Yoko Ogawa, Junko Ogawa, Kazuno Negishi, Jun Shimazaki, Yasuo Sakamoto, Hiroshi Sasaki, Kazuo Tsubota

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Abstract

PURPOSE. To investigate the lipid and DNA oxidative stress as well as corneal and retinal effects after ultraviolet B (UV-B) exposure in mice, with or without silicon hydrogel soft contact lenses (SCL). METHODS. Twenty-eight C57BL6-strain male mice were divided into four groups: group I, control group with no SCL (SCL [-]) and no UV-B exposure (UV-B [-]); group II, senofilcon A SCL (senofilcon [+]) with UV-B exposure (UV-B [+]); group III, lotrafilcon A SCL (lotrafilcon [+]) with UV-B exposure (UV-B [+]); and group IV, no SCL (SCL [-]), but with UV-B exposure (UV-B [+]). All mice except group I received UV-B exposure for 5 days for a total dose of 2.73 J/cm 2. All mice underwent tear hexanoyl-lysine (HEL) and tear cytokine ELISA measurements, and fluorescein and rose bengal corneal staining before and after UV-B exposure. Corneal specimens underwent immunohistochemistry staining with CD45, HEL, 4-hydroxynonenal (4-HNE), and 8-hydroxy-20-deoxyguanosine (8-OHdG) antibodies and evaluation with electron microscopy. RESULTS. All mice without SCL but exposed to UV-B developed corneal edema, ulcers, or epithelial damage compared with mice with senofilcon A SCL and exposure to UV-B. Tear HEL and cytokine levels significantly increased in mice without SCL after UV-B exposure. Immunohistochemistry showed a significantly higher number of cells positively stained for CD45, 8-OHdG, HEL, and 4-HNE in the corneas of mice without SCLs compared with those with senofilcon A after UV-B exposure. CONCLUSION. Silicon hydrogel SCL showed corneal and retinal protective effects, owing to UV blocking properties, against oxidative stress-related membrane lipid and cellular DNA damage.

Original languageEnglish
Pages (from-to)2403-2413
Number of pages11
JournalInvestigative Ophthalmology and Visual Science
Volume53
Issue number4
DOIs
Publication statusPublished - 2012 Apr

Fingerprint

Hydrophilic Contact Lens
Lysine
Tears
Hydrogel
Silicon
Oxidative Stress
Immunohistochemistry
Staining and Labeling
Cytokines
Corneal Edema
Corneal Ulcer
Rose Bengal
Deoxyguanosine
Membrane Lipids
Fluorescein
Cornea
DNA Damage
Electron Microscopy
Cell Count
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Corneal and retinal effects of ultraviolet-B exposure in a soft contact lens mouse model. / Ibrahim, Osama M A; Kojima, Takashi; Wakamatsu, Tais Hitomi; Dogru, Murat; Matsumoto, Yukihiro; Ogawa, Yoko; Ogawa, Junko; Negishi, Kazuno; Shimazaki, Jun; Sakamoto, Yasuo; Sasaki, Hiroshi; Tsubota, Kazuo.

In: Investigative Ophthalmology and Visual Science, Vol. 53, No. 4, 04.2012, p. 2403-2413.

Research output: Contribution to journalArticle

Ibrahim, OMA, Kojima, T, Wakamatsu, TH, Dogru, M, Matsumoto, Y, Ogawa, Y, Ogawa, J, Negishi, K, Shimazaki, J, Sakamoto, Y, Sasaki, H & Tsubota, K 2012, 'Corneal and retinal effects of ultraviolet-B exposure in a soft contact lens mouse model', Investigative Ophthalmology and Visual Science, vol. 53, no. 4, pp. 2403-2413. https://doi.org/10.1167/iovs.11-6863
Ibrahim, Osama M A ; Kojima, Takashi ; Wakamatsu, Tais Hitomi ; Dogru, Murat ; Matsumoto, Yukihiro ; Ogawa, Yoko ; Ogawa, Junko ; Negishi, Kazuno ; Shimazaki, Jun ; Sakamoto, Yasuo ; Sasaki, Hiroshi ; Tsubota, Kazuo. / Corneal and retinal effects of ultraviolet-B exposure in a soft contact lens mouse model. In: Investigative Ophthalmology and Visual Science. 2012 ; Vol. 53, No. 4. pp. 2403-2413.
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abstract = "PURPOSE. To investigate the lipid and DNA oxidative stress as well as corneal and retinal effects after ultraviolet B (UV-B) exposure in mice, with or without silicon hydrogel soft contact lenses (SCL). METHODS. Twenty-eight C57BL6-strain male mice were divided into four groups: group I, control group with no SCL (SCL [-]) and no UV-B exposure (UV-B [-]); group II, senofilcon A SCL (senofilcon [+]) with UV-B exposure (UV-B [+]); group III, lotrafilcon A SCL (lotrafilcon [+]) with UV-B exposure (UV-B [+]); and group IV, no SCL (SCL [-]), but with UV-B exposure (UV-B [+]). All mice except group I received UV-B exposure for 5 days for a total dose of 2.73 J/cm 2. All mice underwent tear hexanoyl-lysine (HEL) and tear cytokine ELISA measurements, and fluorescein and rose bengal corneal staining before and after UV-B exposure. Corneal specimens underwent immunohistochemistry staining with CD45, HEL, 4-hydroxynonenal (4-HNE), and 8-hydroxy-20-deoxyguanosine (8-OHdG) antibodies and evaluation with electron microscopy. RESULTS. All mice without SCL but exposed to UV-B developed corneal edema, ulcers, or epithelial damage compared with mice with senofilcon A SCL and exposure to UV-B. Tear HEL and cytokine levels significantly increased in mice without SCL after UV-B exposure. Immunohistochemistry showed a significantly higher number of cells positively stained for CD45, 8-OHdG, HEL, and 4-HNE in the corneas of mice without SCLs compared with those with senofilcon A after UV-B exposure. CONCLUSION. Silicon hydrogel SCL showed corneal and retinal protective effects, owing to UV blocking properties, against oxidative stress-related membrane lipid and cellular DNA damage.",
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T1 - Corneal and retinal effects of ultraviolet-B exposure in a soft contact lens mouse model

AU - Ibrahim, Osama M A

AU - Kojima, Takashi

AU - Wakamatsu, Tais Hitomi

AU - Dogru, Murat

AU - Matsumoto, Yukihiro

AU - Ogawa, Yoko

AU - Ogawa, Junko

AU - Negishi, Kazuno

AU - Shimazaki, Jun

AU - Sakamoto, Yasuo

AU - Sasaki, Hiroshi

AU - Tsubota, Kazuo

PY - 2012/4

Y1 - 2012/4

N2 - PURPOSE. To investigate the lipid and DNA oxidative stress as well as corneal and retinal effects after ultraviolet B (UV-B) exposure in mice, with or without silicon hydrogel soft contact lenses (SCL). METHODS. Twenty-eight C57BL6-strain male mice were divided into four groups: group I, control group with no SCL (SCL [-]) and no UV-B exposure (UV-B [-]); group II, senofilcon A SCL (senofilcon [+]) with UV-B exposure (UV-B [+]); group III, lotrafilcon A SCL (lotrafilcon [+]) with UV-B exposure (UV-B [+]); and group IV, no SCL (SCL [-]), but with UV-B exposure (UV-B [+]). All mice except group I received UV-B exposure for 5 days for a total dose of 2.73 J/cm 2. All mice underwent tear hexanoyl-lysine (HEL) and tear cytokine ELISA measurements, and fluorescein and rose bengal corneal staining before and after UV-B exposure. Corneal specimens underwent immunohistochemistry staining with CD45, HEL, 4-hydroxynonenal (4-HNE), and 8-hydroxy-20-deoxyguanosine (8-OHdG) antibodies and evaluation with electron microscopy. RESULTS. All mice without SCL but exposed to UV-B developed corneal edema, ulcers, or epithelial damage compared with mice with senofilcon A SCL and exposure to UV-B. Tear HEL and cytokine levels significantly increased in mice without SCL after UV-B exposure. Immunohistochemistry showed a significantly higher number of cells positively stained for CD45, 8-OHdG, HEL, and 4-HNE in the corneas of mice without SCLs compared with those with senofilcon A after UV-B exposure. CONCLUSION. Silicon hydrogel SCL showed corneal and retinal protective effects, owing to UV blocking properties, against oxidative stress-related membrane lipid and cellular DNA damage.

AB - PURPOSE. To investigate the lipid and DNA oxidative stress as well as corneal and retinal effects after ultraviolet B (UV-B) exposure in mice, with or without silicon hydrogel soft contact lenses (SCL). METHODS. Twenty-eight C57BL6-strain male mice were divided into four groups: group I, control group with no SCL (SCL [-]) and no UV-B exposure (UV-B [-]); group II, senofilcon A SCL (senofilcon [+]) with UV-B exposure (UV-B [+]); group III, lotrafilcon A SCL (lotrafilcon [+]) with UV-B exposure (UV-B [+]); and group IV, no SCL (SCL [-]), but with UV-B exposure (UV-B [+]). All mice except group I received UV-B exposure for 5 days for a total dose of 2.73 J/cm 2. All mice underwent tear hexanoyl-lysine (HEL) and tear cytokine ELISA measurements, and fluorescein and rose bengal corneal staining before and after UV-B exposure. Corneal specimens underwent immunohistochemistry staining with CD45, HEL, 4-hydroxynonenal (4-HNE), and 8-hydroxy-20-deoxyguanosine (8-OHdG) antibodies and evaluation with electron microscopy. RESULTS. All mice without SCL but exposed to UV-B developed corneal edema, ulcers, or epithelial damage compared with mice with senofilcon A SCL and exposure to UV-B. Tear HEL and cytokine levels significantly increased in mice without SCL after UV-B exposure. Immunohistochemistry showed a significantly higher number of cells positively stained for CD45, 8-OHdG, HEL, and 4-HNE in the corneas of mice without SCLs compared with those with senofilcon A after UV-B exposure. CONCLUSION. Silicon hydrogel SCL showed corneal and retinal protective effects, owing to UV blocking properties, against oxidative stress-related membrane lipid and cellular DNA damage.

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