Corneal epithelium following penetrating keratoplasty

Kazuo Tsubota, Y. Mashima, H. Murata, M. Yamada, N. Sato

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Aims - This study was designed to observe any changes to the corneal epithelium after penetrating keratoplasty. Methods - The corneal epithelia of 26 patients were observed by specular microscopy 1 week, 1 month, 3 months, and 6 months following penetrating keratoplasty. Results - After re-epithelialisation was confirmed by biomicroscopy 1 week after surgery, specular microscopy revealed many abnormal cells, including spindle shaped cells, nucleated cells, large cells, as well as irregular cell configurations. Although these abnormal findings tended to decrease with time, they were still present in some cases as much as 6 months postoperatively. Computerised morphometric analysis yielded mean cell areas of 1121 (SD 168) (μm2, 1139 (675) μm2, 1712 (496) μm2, and 1400 (377) μm2 at 1 week, 1 month, 3 months, and 6 months respectively, all significantly greater than that of age matched controls (710 (151) μm2). The shape factor decreased with time, but was still greater than the control level at 6 months. Conclusion - This study demonstrates that epithelial abnormalities persist longer than expected after penetrating keratoplasty, and that these subtle changes can be detected by specular microscopic observation, potentially allowing for modification and enhancement of the wound healing process.

Original languageEnglish
Pages (from-to)257-260
Number of pages4
JournalBritish Journal of Ophthalmology
Volume79
Issue number3
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Penetrating Keratoplasty
Corneal Epithelium
Microscopy
Re-Epithelialization
Wound Healing

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Tsubota, K., Mashima, Y., Murata, H., Yamada, M., & Sato, N. (1995). Corneal epithelium following penetrating keratoplasty. British Journal of Ophthalmology, 79(3), 257-260.

Corneal epithelium following penetrating keratoplasty. / Tsubota, Kazuo; Mashima, Y.; Murata, H.; Yamada, M.; Sato, N.

In: British Journal of Ophthalmology, Vol. 79, No. 3, 1995, p. 257-260.

Research output: Contribution to journalArticle

Tsubota, K, Mashima, Y, Murata, H, Yamada, M & Sato, N 1995, 'Corneal epithelium following penetrating keratoplasty', British Journal of Ophthalmology, vol. 79, no. 3, pp. 257-260.
Tsubota K, Mashima Y, Murata H, Yamada M, Sato N. Corneal epithelium following penetrating keratoplasty. British Journal of Ophthalmology. 1995;79(3):257-260.
Tsubota, Kazuo ; Mashima, Y. ; Murata, H. ; Yamada, M. ; Sato, N. / Corneal epithelium following penetrating keratoplasty. In: British Journal of Ophthalmology. 1995 ; Vol. 79, No. 3. pp. 257-260.
@article{1cc383e0a6f0476ab869f82f9a1d963b,
title = "Corneal epithelium following penetrating keratoplasty",
abstract = "Aims - This study was designed to observe any changes to the corneal epithelium after penetrating keratoplasty. Methods - The corneal epithelia of 26 patients were observed by specular microscopy 1 week, 1 month, 3 months, and 6 months following penetrating keratoplasty. Results - After re-epithelialisation was confirmed by biomicroscopy 1 week after surgery, specular microscopy revealed many abnormal cells, including spindle shaped cells, nucleated cells, large cells, as well as irregular cell configurations. Although these abnormal findings tended to decrease with time, they were still present in some cases as much as 6 months postoperatively. Computerised morphometric analysis yielded mean cell areas of 1121 (SD 168) (μm2, 1139 (675) μm2, 1712 (496) μm2, and 1400 (377) μm2 at 1 week, 1 month, 3 months, and 6 months respectively, all significantly greater than that of age matched controls (710 (151) μm2). The shape factor decreased with time, but was still greater than the control level at 6 months. Conclusion - This study demonstrates that epithelial abnormalities persist longer than expected after penetrating keratoplasty, and that these subtle changes can be detected by specular microscopic observation, potentially allowing for modification and enhancement of the wound healing process.",
author = "Kazuo Tsubota and Y. Mashima and H. Murata and M. Yamada and N. Sato",
year = "1995",
language = "English",
volume = "79",
pages = "257--260",
journal = "British Journal of Ophthalmology",
issn = "0007-1161",
publisher = "BMJ Publishing Group",
number = "3",

}

TY - JOUR

T1 - Corneal epithelium following penetrating keratoplasty

AU - Tsubota, Kazuo

AU - Mashima, Y.

AU - Murata, H.

AU - Yamada, M.

AU - Sato, N.

PY - 1995

Y1 - 1995

N2 - Aims - This study was designed to observe any changes to the corneal epithelium after penetrating keratoplasty. Methods - The corneal epithelia of 26 patients were observed by specular microscopy 1 week, 1 month, 3 months, and 6 months following penetrating keratoplasty. Results - After re-epithelialisation was confirmed by biomicroscopy 1 week after surgery, specular microscopy revealed many abnormal cells, including spindle shaped cells, nucleated cells, large cells, as well as irregular cell configurations. Although these abnormal findings tended to decrease with time, they were still present in some cases as much as 6 months postoperatively. Computerised morphometric analysis yielded mean cell areas of 1121 (SD 168) (μm2, 1139 (675) μm2, 1712 (496) μm2, and 1400 (377) μm2 at 1 week, 1 month, 3 months, and 6 months respectively, all significantly greater than that of age matched controls (710 (151) μm2). The shape factor decreased with time, but was still greater than the control level at 6 months. Conclusion - This study demonstrates that epithelial abnormalities persist longer than expected after penetrating keratoplasty, and that these subtle changes can be detected by specular microscopic observation, potentially allowing for modification and enhancement of the wound healing process.

AB - Aims - This study was designed to observe any changes to the corneal epithelium after penetrating keratoplasty. Methods - The corneal epithelia of 26 patients were observed by specular microscopy 1 week, 1 month, 3 months, and 6 months following penetrating keratoplasty. Results - After re-epithelialisation was confirmed by biomicroscopy 1 week after surgery, specular microscopy revealed many abnormal cells, including spindle shaped cells, nucleated cells, large cells, as well as irregular cell configurations. Although these abnormal findings tended to decrease with time, they were still present in some cases as much as 6 months postoperatively. Computerised morphometric analysis yielded mean cell areas of 1121 (SD 168) (μm2, 1139 (675) μm2, 1712 (496) μm2, and 1400 (377) μm2 at 1 week, 1 month, 3 months, and 6 months respectively, all significantly greater than that of age matched controls (710 (151) μm2). The shape factor decreased with time, but was still greater than the control level at 6 months. Conclusion - This study demonstrates that epithelial abnormalities persist longer than expected after penetrating keratoplasty, and that these subtle changes can be detected by specular microscopic observation, potentially allowing for modification and enhancement of the wound healing process.

UR - http://www.scopus.com/inward/record.url?scp=0028941918&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028941918&partnerID=8YFLogxK

M3 - Article

VL - 79

SP - 257

EP - 260

JO - British Journal of Ophthalmology

JF - British Journal of Ophthalmology

SN - 0007-1161

IS - 3

ER -