Correlation between Reversing of Multidrug Resistance and Inhibiting of [3H]Azidopine Photolabeling of P-Glycoprotein by Newly Synthesized Dihydropyridine Analogues in a Human Cell Line

Hiroshi Kikuchi, Akihiko Yoshimura, Tomoyuki Sumizawa, Shin ichi Akiyama, Mikio Kamiwatari, Yukihiro Nagata, Norimasa Shudo, Ryozo Sakoda, Kiyotomo Seto

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45 Citations (Scopus)

Abstract

Ten synthetic dihydropyridine analogues were investigated for their ability to reverse drug resistance in a multidrug-resistant human carcinoma cell line, KB-C1. Four dihydropyridine analogues completely reversed the resistance, three lowered the resistance, and three had little effect. The radioactive photoactive dihydropyridine calcium channel blocker, |3H]azidopine, photolabels P-glycoprotein in membrane vesicles from KB-Cl cells. This photolabeling was almost completely inhibited by excess dihydropyridine analogues that reversed or lowered drug resistance. In contrast, the labeling was not significantly inhibited by analogues that do not reverse resistance. Among other reversing agents, cepharanthine and reserpine inhibited the [3H]azidopine photolabeling, but thioridazine did not. N-Solanesyl-N,N′-bis(3,4-dimethoxybenzyl)ethylenediamine slightly inhibited the labeling at 100 μM. An anticancer agent, vinblastine, also inhibited the labeling. The correlation between the reversing of the drug resistance and the inhibition of the [3H|azidopine photolabeling of P-glycoprotein by dihydropyridine analogues suggests a role for P-glycoprotein in multidrug resistance and also the reversing of the resistance by dihydropyridine analogues.

Original languageEnglish
Pages (from-to)3190-3195
Number of pages6
JournalCancer Research
Volume49
Issue number12
Publication statusPublished - 1989 Jun 15
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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