Abstract
The rnh gene of Escherichia coli encodes RNase H. rnh mutants display at least two phenotypes: (1) they require functional RecBCD enzyme for growth; thus rnh-339::cat recB270 (Ts) and rnh-339::cat recC271 (Ts) strains are temperature sensitive for growth; (2) rnh mutants permit replication that is independent of the chromosomal origin, presumably by failing to remove RNA-DNA hybrids from which extra-original replication can be primed. We report here that manifestation of these two phenotypes occurs at different levels of RNase H function; we have examined partially functional rnh mutants for their in vitro RNase H activity, their ability to rescue viability in recB or recC cells and their ability to permit growth of mutants incapable of using oriC [dnaA (Ts)].
Original language | English |
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Pages (from-to) | 433-437 |
Number of pages | 5 |
Journal | MGG Molecular & General Genetics |
Volume | 227 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1991 Jul 1 |
Keywords
- RNase H
- dnaA
- recBCD
ASJC Scopus subject areas
- Genetics