Correlation of in vivo nitric oxide and cGMP with glutamate/glutamine metabolism in the rat striatum

Kouichi Ohta, Nobuo Araki, Mamoru Shibata, Junichi Hamada, Satoru Komatsumoto, Kunio Shimazu, Yasuo Fukuuchi

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13 Citations (Scopus)

Abstract

We have examined how the suppression of endogenous production of nitric oxide (NO) in the striatal tissue affects release of glutamate (GLU) and glutamine (GLN) in pentobarbital-anesthetized male Sprague-Dawley rats. For the quantitative measurement of tissue NO production and amino acid release, an in vivo assay system for extracellular nitrite (NO2-) and amino acids was employed using an in vivo microdialysis technique. An NO synthase inhibitor (N(G)-nitro-L-arginine methyl ester, L-NAME) in concentrations ranging between 4-40 mM was perfused into the rat striatum using the assay system. Tissue NO production was found to be inversely proportional to the L-NAME concentration. L-NAME likewise decreased striatal levels of GLU and GLN. Furthermore, tissue NO production showed a positive correlation with GLU (R = 0.62, P < 0.02) and GLN (R = 0.86, P < 0.001) concentrations. Exogenous application of NO and cGMP by intrastriatal perfusion with 0.1-2.5 mM hydroxylamine and 0.4-10 mM 8-bromo-cGMP, respectively, increased striatal GLU release in a dose-related manner. Hydroxylamine reduced GLN release, and 8-bromo-cGMP showed a tendency to decrease GLN. In conclusion, striatal GLU/GLN metabolism is a function of the tissue concentration of NO. Normal endogenous concentration of NO causes GLU to be released at a consistent basal level, and enhanced tissue NO production facilitates GLU release via pathways including cGMP formation. We hypothesize that NO may suppress GLN formation by astrocytes.

Original languageEnglish
Pages (from-to)379-384
Number of pages6
JournalNeuroscience Research
Volume25
Issue number4
DOIs
Publication statusPublished - 1996 Aug

Keywords

  • 8-bromoguanosine 3':5'-cyclic monophosphate
  • Excitatory amino acid
  • Glutamine synthase
  • Hydroxylamine
  • In vivo microdialysis
  • N(G)-nitro-L-arginine methyl ester
  • Neurotransmitter
  • Nitric oxide synthase

ASJC Scopus subject areas

  • Neuroscience(all)

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  • Cite this

    Ohta, K., Araki, N., Shibata, M., Hamada, J., Komatsumoto, S., Shimazu, K., & Fukuuchi, Y. (1996). Correlation of in vivo nitric oxide and cGMP with glutamate/glutamine metabolism in the rat striatum. Neuroscience Research, 25(4), 379-384. https://doi.org/10.1016/S0168-0102(96)01064-4