Abstract
As human papillomavirus (HPV) infection is the main risk factor for squamous cell carcinoma of the cervix and overexpression of p16INK4a occurs when retinoblastoma protein is inactivated by high-risk HPV, the authors studied the association of HPV infection and expression of p16INK4a in cervical adenocarcinomas. Specimens of cervical glandular neoplasias were immunostained with a p16INK4a-specific monoclonal antibody (clone E6H4). Approximately 80% of glandular neoplasms showed overexpression of p16INK4a. Exfoliated cells from 14 adenocarcinomas were further examined by p16INK4a-specific immunocytochemistry, and 12 cases showed overexpression of p16INK4a, suggesting that immunostaining for p16INK4a may be a useful diagnostic tool for cervical adenocarcinomas. The authors further examined HPV DNA in cervical adenocarcinomas with the polymerase chain reaction method. Overexpression of p16INK4a was positive in 94% of cases in which HPV16 or 18DNA was positive, a finding suggesting that HPV16 or 18 may play an important role in cervical adenocarcinomas. Overexpression of p16INK4a may be an indicator of pathogenic activity of high-risk HPVs.
| Original language | English |
|---|---|
| Pages (from-to) | 378-385 |
| Number of pages | 8 |
| Journal | International Journal of Gynecological Pathology |
| Volume | 22 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2003 Oct |
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Keywords
- Adenocarcinoma
- Human papillomavirus
- P16
- Uterine cervix
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Obstetrics and Gynaecology
Cite this
Correlation of p16INK4A overexpression with human papillomavirus infection in cervical adenocarcinomas. / Ishikawa, Mitsuya; Fujii, Takuma; Masumoto, Nobuo; Saito, Miyuki; Mukai, Makio; Nindl, Ingo; Ridder, Ruediger; Fukuchi, Takeshi; Kubushiro, Kaneyuki; Tsukazaki, Katsumi; Nozawa, Shiro.
In: International Journal of Gynecological Pathology, Vol. 22, No. 4, 10.2003, p. 378-385.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Correlation of p16INK4A overexpression with human papillomavirus infection in cervical adenocarcinomas
AU - Ishikawa, Mitsuya
AU - Fujii, Takuma
AU - Masumoto, Nobuo
AU - Saito, Miyuki
AU - Mukai, Makio
AU - Nindl, Ingo
AU - Ridder, Ruediger
AU - Fukuchi, Takeshi
AU - Kubushiro, Kaneyuki
AU - Tsukazaki, Katsumi
AU - Nozawa, Shiro
PY - 2003/10
Y1 - 2003/10
N2 - As human papillomavirus (HPV) infection is the main risk factor for squamous cell carcinoma of the cervix and overexpression of p16INK4a occurs when retinoblastoma protein is inactivated by high-risk HPV, the authors studied the association of HPV infection and expression of p16INK4a in cervical adenocarcinomas. Specimens of cervical glandular neoplasias were immunostained with a p16INK4a-specific monoclonal antibody (clone E6H4). Approximately 80% of glandular neoplasms showed overexpression of p16INK4a. Exfoliated cells from 14 adenocarcinomas were further examined by p16INK4a-specific immunocytochemistry, and 12 cases showed overexpression of p16INK4a, suggesting that immunostaining for p16INK4a may be a useful diagnostic tool for cervical adenocarcinomas. The authors further examined HPV DNA in cervical adenocarcinomas with the polymerase chain reaction method. Overexpression of p16INK4a was positive in 94% of cases in which HPV16 or 18DNA was positive, a finding suggesting that HPV16 or 18 may play an important role in cervical adenocarcinomas. Overexpression of p16INK4a may be an indicator of pathogenic activity of high-risk HPVs.
AB - As human papillomavirus (HPV) infection is the main risk factor for squamous cell carcinoma of the cervix and overexpression of p16INK4a occurs when retinoblastoma protein is inactivated by high-risk HPV, the authors studied the association of HPV infection and expression of p16INK4a in cervical adenocarcinomas. Specimens of cervical glandular neoplasias were immunostained with a p16INK4a-specific monoclonal antibody (clone E6H4). Approximately 80% of glandular neoplasms showed overexpression of p16INK4a. Exfoliated cells from 14 adenocarcinomas were further examined by p16INK4a-specific immunocytochemistry, and 12 cases showed overexpression of p16INK4a, suggesting that immunostaining for p16INK4a may be a useful diagnostic tool for cervical adenocarcinomas. The authors further examined HPV DNA in cervical adenocarcinomas with the polymerase chain reaction method. Overexpression of p16INK4a was positive in 94% of cases in which HPV16 or 18DNA was positive, a finding suggesting that HPV16 or 18 may play an important role in cervical adenocarcinomas. Overexpression of p16INK4a may be an indicator of pathogenic activity of high-risk HPVs.
KW - Adenocarcinoma
KW - Human papillomavirus
KW - P16
KW - Uterine cervix
UR - http://www.scopus.com/inward/record.url?scp=0141569547&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0141569547&partnerID=8YFLogxK
U2 - 10.1097/01.pgp.0000092135.88121.1f
DO - 10.1097/01.pgp.0000092135.88121.1f
M3 - Article
VL - 22
SP - 378
EP - 385
JO - International Journal of Gynecological Pathology
JF - International Journal of Gynecological Pathology
SN - 0277-1691
IS - 4
ER -