Correlation of postoperative recurrence in hepatocellular carcinoma with demethylation of repetitive sequences

Osamu Itano, Masakazu Ueda, Kiyoshi Kikuchi, Osamu Hashimoto, Shigeo Hayatsu, Masaharu Kawaguchi, Hiroaki Seki, Kouichi Aiura, Masaki Kitajima

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Restriction landmark genomic scanning (RLGS) was utilized to identify novel genomic alterations in hepatocellular carcinoma (HCC). Thirty-one HCC samples were examined by RLGS. Two high intensity spots were common to several RLGS profiles of different HCCs. Nucleotide sequencing and homology search analysis showed that these spots represented repetitive sequences, Human tandem repeat sequence (Genbank, L09552) and centromeric NotI cluster (Genbank, Y10752). These intensified signals were attributable to the occurrence of demethylated areas in the recognition sequence of the NotI site of the corresponding fragments. The intensity of these spots in the RLGS profile reflects their degree of demethylation, which was significantly correlated with postoperative recurrence, even in patients regarded as belonging to the good prognosis group by conventional prognostic factors. Multivariate analysis showed that the intensities of the two spots retained independent prognostic value. This is a new type of predictive factor for HCC based on epigenetic changes in hepatocarcinogenesis, and in the future it is expected to be of great value in making preoperative diagnosis and selecting postoperative therapy.

Original languageEnglish
Pages (from-to)789-797
Number of pages9
JournalOncogene
Volume21
Issue number5
DOIs
Publication statusPublished - 2002 Jan 24

Fingerprint

Nucleic Acid Repetitive Sequences
Hepatocellular Carcinoma
Nucleic Acid Databases
Recurrence
Tandem Repeat Sequences
Epigenomics
Multivariate Analysis
Nucleotides
Therapeutics

Keywords

  • Demethylation
  • Hepatocellular carcinoma
  • Postoperative recurrence
  • Repetitive sequence
  • Restriction landmark genomic scanning

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Itano, O., Ueda, M., Kikuchi, K., Hashimoto, O., Hayatsu, S., Kawaguchi, M., ... Kitajima, M. (2002). Correlation of postoperative recurrence in hepatocellular carcinoma with demethylation of repetitive sequences. Oncogene, 21(5), 789-797. https://doi.org/10.1038/sj/onc/1205124

Correlation of postoperative recurrence in hepatocellular carcinoma with demethylation of repetitive sequences. / Itano, Osamu; Ueda, Masakazu; Kikuchi, Kiyoshi; Hashimoto, Osamu; Hayatsu, Shigeo; Kawaguchi, Masaharu; Seki, Hiroaki; Aiura, Kouichi; Kitajima, Masaki.

In: Oncogene, Vol. 21, No. 5, 24.01.2002, p. 789-797.

Research output: Contribution to journalArticle

Itano, O, Ueda, M, Kikuchi, K, Hashimoto, O, Hayatsu, S, Kawaguchi, M, Seki, H, Aiura, K & Kitajima, M 2002, 'Correlation of postoperative recurrence in hepatocellular carcinoma with demethylation of repetitive sequences', Oncogene, vol. 21, no. 5, pp. 789-797. https://doi.org/10.1038/sj/onc/1205124
Itano, Osamu ; Ueda, Masakazu ; Kikuchi, Kiyoshi ; Hashimoto, Osamu ; Hayatsu, Shigeo ; Kawaguchi, Masaharu ; Seki, Hiroaki ; Aiura, Kouichi ; Kitajima, Masaki. / Correlation of postoperative recurrence in hepatocellular carcinoma with demethylation of repetitive sequences. In: Oncogene. 2002 ; Vol. 21, No. 5. pp. 789-797.
@article{9ae032d1935d4f4eb40cddbd09e1d77a,
title = "Correlation of postoperative recurrence in hepatocellular carcinoma with demethylation of repetitive sequences",
abstract = "Restriction landmark genomic scanning (RLGS) was utilized to identify novel genomic alterations in hepatocellular carcinoma (HCC). Thirty-one HCC samples were examined by RLGS. Two high intensity spots were common to several RLGS profiles of different HCCs. Nucleotide sequencing and homology search analysis showed that these spots represented repetitive sequences, Human tandem repeat sequence (Genbank, L09552) and centromeric NotI cluster (Genbank, Y10752). These intensified signals were attributable to the occurrence of demethylated areas in the recognition sequence of the NotI site of the corresponding fragments. The intensity of these spots in the RLGS profile reflects their degree of demethylation, which was significantly correlated with postoperative recurrence, even in patients regarded as belonging to the good prognosis group by conventional prognostic factors. Multivariate analysis showed that the intensities of the two spots retained independent prognostic value. This is a new type of predictive factor for HCC based on epigenetic changes in hepatocarcinogenesis, and in the future it is expected to be of great value in making preoperative diagnosis and selecting postoperative therapy.",
keywords = "Demethylation, Hepatocellular carcinoma, Postoperative recurrence, Repetitive sequence, Restriction landmark genomic scanning",
author = "Osamu Itano and Masakazu Ueda and Kiyoshi Kikuchi and Osamu Hashimoto and Shigeo Hayatsu and Masaharu Kawaguchi and Hiroaki Seki and Kouichi Aiura and Masaki Kitajima",
year = "2002",
month = "1",
day = "24",
doi = "10.1038/sj/onc/1205124",
language = "English",
volume = "21",
pages = "789--797",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "5",

}

TY - JOUR

T1 - Correlation of postoperative recurrence in hepatocellular carcinoma with demethylation of repetitive sequences

AU - Itano, Osamu

AU - Ueda, Masakazu

AU - Kikuchi, Kiyoshi

AU - Hashimoto, Osamu

AU - Hayatsu, Shigeo

AU - Kawaguchi, Masaharu

AU - Seki, Hiroaki

AU - Aiura, Kouichi

AU - Kitajima, Masaki

PY - 2002/1/24

Y1 - 2002/1/24

N2 - Restriction landmark genomic scanning (RLGS) was utilized to identify novel genomic alterations in hepatocellular carcinoma (HCC). Thirty-one HCC samples were examined by RLGS. Two high intensity spots were common to several RLGS profiles of different HCCs. Nucleotide sequencing and homology search analysis showed that these spots represented repetitive sequences, Human tandem repeat sequence (Genbank, L09552) and centromeric NotI cluster (Genbank, Y10752). These intensified signals were attributable to the occurrence of demethylated areas in the recognition sequence of the NotI site of the corresponding fragments. The intensity of these spots in the RLGS profile reflects their degree of demethylation, which was significantly correlated with postoperative recurrence, even in patients regarded as belonging to the good prognosis group by conventional prognostic factors. Multivariate analysis showed that the intensities of the two spots retained independent prognostic value. This is a new type of predictive factor for HCC based on epigenetic changes in hepatocarcinogenesis, and in the future it is expected to be of great value in making preoperative diagnosis and selecting postoperative therapy.

AB - Restriction landmark genomic scanning (RLGS) was utilized to identify novel genomic alterations in hepatocellular carcinoma (HCC). Thirty-one HCC samples were examined by RLGS. Two high intensity spots were common to several RLGS profiles of different HCCs. Nucleotide sequencing and homology search analysis showed that these spots represented repetitive sequences, Human tandem repeat sequence (Genbank, L09552) and centromeric NotI cluster (Genbank, Y10752). These intensified signals were attributable to the occurrence of demethylated areas in the recognition sequence of the NotI site of the corresponding fragments. The intensity of these spots in the RLGS profile reflects their degree of demethylation, which was significantly correlated with postoperative recurrence, even in patients regarded as belonging to the good prognosis group by conventional prognostic factors. Multivariate analysis showed that the intensities of the two spots retained independent prognostic value. This is a new type of predictive factor for HCC based on epigenetic changes in hepatocarcinogenesis, and in the future it is expected to be of great value in making preoperative diagnosis and selecting postoperative therapy.

KW - Demethylation

KW - Hepatocellular carcinoma

KW - Postoperative recurrence

KW - Repetitive sequence

KW - Restriction landmark genomic scanning

UR - http://www.scopus.com/inward/record.url?scp=0037165224&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037165224&partnerID=8YFLogxK

U2 - 10.1038/sj/onc/1205124

DO - 10.1038/sj/onc/1205124

M3 - Article

C2 - 11850807

AN - SCOPUS:0037165224

VL - 21

SP - 789

EP - 797

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 5

ER -