TY - JOUR
T1 - cortex
AU - Murata, Rei
AU - Takada, Yuki
AU - Takuwa, Hiroyuki
AU - Kawaguchi, Hiroshi
AU - Ito, Hiroshi
AU - Kanno, Iwao
AU - Tottori, Naotomo
AU - Yamada, Yukio
AU - Tomita, Yutaka
AU - Itoh, Yoshiaki
AU - Suzuki, Norihiro
AU - Yamada, Katsuya
AU - Masamoto, Kazuto
N1 - Funding Information:
This study was partially supported through funding from JSPS KAKENHI (#25750400) (to K.M.), JSPS KEKENHI (#24659578) (to I.K.), and a grant from the Ministry of Health, Labor and Welfare (MHLW), Japan (to I.K.).
Publisher Copyright:
© Springer Science+Business Media, LLC 2014.
PY - 2014
Y1 - 2014
N2 - The present study examined glucose transfer in the cellular scale of mouse brain microvasculature in vivo using two-photon microscopy and fl uorescent glucose analogue (2-NBDG). The 2-NBDG was intravenously injected (0.04 mL/ min) in the anesthetized Tie2-GFP mice in which the vascular endothelium expressed fl uorescent protein. Time-lapse imaging was conducted on the cortical parenchyma, while the time–intensity change of the injected 2-NBDG was analysed in respective vascular compartments (artery, capillary, and vein). We observed that 2-NBDG signal increased monotonically in the vasculature during the period of the injection, and rapidly declined following its cessation. In tissue compartment, however, the signal intensity gradually increased even after cessation of the injection. Spatiotemporal analysis of the 2-NBDG intensity over the cross-sections of the vessels further showed distinct change of the 2-NBDG intensity across the vessel wall (endothelium), which may represents a regulation site of tissue glucose infl ux.
AB - The present study examined glucose transfer in the cellular scale of mouse brain microvasculature in vivo using two-photon microscopy and fl uorescent glucose analogue (2-NBDG). The 2-NBDG was intravenously injected (0.04 mL/ min) in the anesthetized Tie2-GFP mice in which the vascular endothelium expressed fl uorescent protein. Time-lapse imaging was conducted on the cortical parenchyma, while the time–intensity change of the injected 2-NBDG was analysed in respective vascular compartments (artery, capillary, and vein). We observed that 2-NBDG signal increased monotonically in the vasculature during the period of the injection, and rapidly declined following its cessation. In tissue compartment, however, the signal intensity gradually increased even after cessation of the injection. Spatiotemporal analysis of the 2-NBDG intensity over the cross-sections of the vessels further showed distinct change of the 2-NBDG intensity across the vessel wall (endothelium), which may represents a regulation site of tissue glucose infl ux.
KW - 2- NBDG
KW - Glucose transporter
KW - Optical imaging
KW - Two-photon microscopy
KW - Vascular endothelial cell
UR - http://www.scopus.com/inward/record.url?scp=84905904577&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84905904577&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-0620-8_32
DO - 10.1007/978-1-4939-0620-8_32
M3 - Article
C2 - 24729239
AN - SCOPUS:84905904577
SN - 0065-2598
VL - 812
SP - 241
EP - 246
JO - Advances in Experimental Medicine and Biology
JF - Advances in Experimental Medicine and Biology
ER -