cortex

Rei Murata, Yuki Takada, Hiroyuki Takuwa, Hiroshi Kawaguchi, Hiroshi Ito, Iwao Kanno, Naotomo Tottori, Yukio Yamada, Yutaka Tomita, Yoshiaki Itoh, Norihiro Suzuki, Katsuya Yamada, Kazuto Masamoto

Research output: Contribution to journalArticle

Abstract

The present study examined glucose transfer in the cellular scale of mouse brain microvasculature in vivo using two-photon microscopy and fl uorescent glucose analogue (2-NBDG). The 2-NBDG was intravenously injected (0.04 mL/ min) in the anesthetized Tie2-GFP mice in which the vascular endothelium expressed fl uorescent protein. Time-lapse imaging was conducted on the cortical parenchyma, while the time–intensity change of the injected 2-NBDG was analysed in respective vascular compartments (artery, capillary, and vein). We observed that 2-NBDG signal increased monotonically in the vasculature during the period of the injection, and rapidly declined following its cessation. In tissue compartment, however, the signal intensity gradually increased even after cessation of the injection. Spatiotemporal analysis of the 2-NBDG intensity over the cross-sections of the vessels further showed distinct change of the 2-NBDG intensity across the vessel wall (endothelium), which may represents a regulation site of tissue glucose infl ux.

Original languageEnglish
Pages (from-to)241-246
Number of pages6
JournalAdvances in Experimental Medicine and Biology
Volume812
DOIs
Publication statusPublished - 2014

Fingerprint

Glucose
Time-Lapse Imaging
Spatio-Temporal Analysis
Tissue
Injections
Vascular Endothelium
Microvessels
Photons
Endothelium
Blood Vessels
2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose
Microscopy
Veins
Brain
Microscopic examination
Arteries
Imaging techniques
Proteins

Keywords

  • 2- NBDG
  • Glucose transporter
  • Optical imaging
  • Two-photon microscopy
  • Vascular endothelial cell

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Murata, R., Takada, Y., Takuwa, H., Kawaguchi, H., Ito, H., Kanno, I., ... Masamoto, K. (2014). cortex. Advances in Experimental Medicine and Biology, 812, 241-246. https://doi.org/10.1007/978-1-4939-0620-8_32

cortex. / Murata, Rei; Takada, Yuki; Takuwa, Hiroyuki; Kawaguchi, Hiroshi; Ito, Hiroshi; Kanno, Iwao; Tottori, Naotomo; Yamada, Yukio; Tomita, Yutaka; Itoh, Yoshiaki; Suzuki, Norihiro; Yamada, Katsuya; Masamoto, Kazuto.

In: Advances in Experimental Medicine and Biology, Vol. 812, 2014, p. 241-246.

Research output: Contribution to journalArticle

Murata, R, Takada, Y, Takuwa, H, Kawaguchi, H, Ito, H, Kanno, I, Tottori, N, Yamada, Y, Tomita, Y, Itoh, Y, Suzuki, N, Yamada, K & Masamoto, K 2014, ' cortex', Advances in Experimental Medicine and Biology, vol. 812, pp. 241-246. https://doi.org/10.1007/978-1-4939-0620-8_32
Murata R, Takada Y, Takuwa H, Kawaguchi H, Ito H, Kanno I et al. cortex. Advances in Experimental Medicine and Biology. 2014;812:241-246. https://doi.org/10.1007/978-1-4939-0620-8_32
Murata, Rei ; Takada, Yuki ; Takuwa, Hiroyuki ; Kawaguchi, Hiroshi ; Ito, Hiroshi ; Kanno, Iwao ; Tottori, Naotomo ; Yamada, Yukio ; Tomita, Yutaka ; Itoh, Yoshiaki ; Suzuki, Norihiro ; Yamada, Katsuya ; Masamoto, Kazuto. / cortex. In: Advances in Experimental Medicine and Biology. 2014 ; Vol. 812. pp. 241-246.
@article{6e1352af3fcb4ab0b28b4a6e1bf5cd22,
title = "cortex",
abstract = "The present study examined glucose transfer in the cellular scale of mouse brain microvasculature in vivo using two-photon microscopy and fl uorescent glucose analogue (2-NBDG). The 2-NBDG was intravenously injected (0.04 mL/ min) in the anesthetized Tie2-GFP mice in which the vascular endothelium expressed fl uorescent protein. Time-lapse imaging was conducted on the cortical parenchyma, while the time–intensity change of the injected 2-NBDG was analysed in respective vascular compartments (artery, capillary, and vein). We observed that 2-NBDG signal increased monotonically in the vasculature during the period of the injection, and rapidly declined following its cessation. In tissue compartment, however, the signal intensity gradually increased even after cessation of the injection. Spatiotemporal analysis of the 2-NBDG intensity over the cross-sections of the vessels further showed distinct change of the 2-NBDG intensity across the vessel wall (endothelium), which may represents a regulation site of tissue glucose infl ux.",
keywords = "2- NBDG, Glucose transporter, Optical imaging, Two-photon microscopy, Vascular endothelial cell",
author = "Rei Murata and Yuki Takada and Hiroyuki Takuwa and Hiroshi Kawaguchi and Hiroshi Ito and Iwao Kanno and Naotomo Tottori and Yukio Yamada and Yutaka Tomita and Yoshiaki Itoh and Norihiro Suzuki and Katsuya Yamada and Kazuto Masamoto",
year = "2014",
doi = "10.1007/978-1-4939-0620-8_32",
language = "English",
volume = "812",
pages = "241--246",
journal = "Advances in Experimental Medicine and Biology",
issn = "0065-2598",
publisher = "Springer New York",

}

TY - JOUR

T1 - cortex

AU - Murata, Rei

AU - Takada, Yuki

AU - Takuwa, Hiroyuki

AU - Kawaguchi, Hiroshi

AU - Ito, Hiroshi

AU - Kanno, Iwao

AU - Tottori, Naotomo

AU - Yamada, Yukio

AU - Tomita, Yutaka

AU - Itoh, Yoshiaki

AU - Suzuki, Norihiro

AU - Yamada, Katsuya

AU - Masamoto, Kazuto

PY - 2014

Y1 - 2014

N2 - The present study examined glucose transfer in the cellular scale of mouse brain microvasculature in vivo using two-photon microscopy and fl uorescent glucose analogue (2-NBDG). The 2-NBDG was intravenously injected (0.04 mL/ min) in the anesthetized Tie2-GFP mice in which the vascular endothelium expressed fl uorescent protein. Time-lapse imaging was conducted on the cortical parenchyma, while the time–intensity change of the injected 2-NBDG was analysed in respective vascular compartments (artery, capillary, and vein). We observed that 2-NBDG signal increased monotonically in the vasculature during the period of the injection, and rapidly declined following its cessation. In tissue compartment, however, the signal intensity gradually increased even after cessation of the injection. Spatiotemporal analysis of the 2-NBDG intensity over the cross-sections of the vessels further showed distinct change of the 2-NBDG intensity across the vessel wall (endothelium), which may represents a regulation site of tissue glucose infl ux.

AB - The present study examined glucose transfer in the cellular scale of mouse brain microvasculature in vivo using two-photon microscopy and fl uorescent glucose analogue (2-NBDG). The 2-NBDG was intravenously injected (0.04 mL/ min) in the anesthetized Tie2-GFP mice in which the vascular endothelium expressed fl uorescent protein. Time-lapse imaging was conducted on the cortical parenchyma, while the time–intensity change of the injected 2-NBDG was analysed in respective vascular compartments (artery, capillary, and vein). We observed that 2-NBDG signal increased monotonically in the vasculature during the period of the injection, and rapidly declined following its cessation. In tissue compartment, however, the signal intensity gradually increased even after cessation of the injection. Spatiotemporal analysis of the 2-NBDG intensity over the cross-sections of the vessels further showed distinct change of the 2-NBDG intensity across the vessel wall (endothelium), which may represents a regulation site of tissue glucose infl ux.

KW - 2- NBDG

KW - Glucose transporter

KW - Optical imaging

KW - Two-photon microscopy

KW - Vascular endothelial cell

UR - http://www.scopus.com/inward/record.url?scp=84905904577&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84905904577&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-0620-8_32

DO - 10.1007/978-1-4939-0620-8_32

M3 - Article

VL - 812

SP - 241

EP - 246

JO - Advances in Experimental Medicine and Biology

JF - Advances in Experimental Medicine and Biology

SN - 0065-2598

ER -