Cortical microcirculatory disturbance in the super acute phase of subarachnoid hemorrhage - In vivo analysis using two-photon laser scanning microscopy

Mami Ishikawa, Mayumi Kajimura, Takayuki Morikawa, Kosuke Tsukada, Toshiyuki Tsuji, Gen Kusaka, Yuichi Tanaka, Makoto Suematsu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective Subarachnoid hemorrhage (SAH) causes cerebral ischemia and drastically worsens the clinical status at onset. However, the arterial flow is surprisingly well maintained on the cerebral surface. We investigated cortical microcirculatory changes in the super acute phase of SAH using two-photon laser scanning microscopy (TPLSM). Methods SAH was induced at the skull base in 10 mice using a prone endovascular perforation model. Before SAH, and 1, 2, 5, 10, 20, 30 and 60 min after SAH, the cortical microcirculation was observed with TPLSM through a cranial window. Diameters of penetrating and precapillary arterioles were measured and red blood cell (RBC) velocities in precapillary arterioles were analyzed using a line-scan method after administration of Q-dot 655 nanocrystals. Results One minute after SAH, RBC velocity and flow in precapillary arterioles drastically decreased to

Original languageEnglish
Pages (from-to)326-333
Number of pages8
JournalJournal of the Neurological Sciences
Volume368
DOIs
Publication statusPublished - 2016 Sep 15

Fingerprint

Subarachnoid Hemorrhage
Photons
Confocal Microscopy
Arterioles
Erythrocytes
Skull Base
Microcirculation
Brain Ischemia
Nanoparticles

Keywords

  • Ischemia
  • Microcirculation
  • Neurovascular function
  • Subarachnoid hemorrhage
  • Vasodilation

ASJC Scopus subject areas

  • Clinical Neurology
  • Neurology

Cite this

Cortical microcirculatory disturbance in the super acute phase of subarachnoid hemorrhage - In vivo analysis using two-photon laser scanning microscopy. / Ishikawa, Mami; Kajimura, Mayumi; Morikawa, Takayuki; Tsukada, Kosuke; Tsuji, Toshiyuki; Kusaka, Gen; Tanaka, Yuichi; Suematsu, Makoto.

In: Journal of the Neurological Sciences, Vol. 368, 15.09.2016, p. 326-333.

Research output: Contribution to journalArticle

@article{9d3598c8468b43da802a0260ad0cb415,
title = "Cortical microcirculatory disturbance in the super acute phase of subarachnoid hemorrhage - In vivo analysis using two-photon laser scanning microscopy",
abstract = "Objective Subarachnoid hemorrhage (SAH) causes cerebral ischemia and drastically worsens the clinical status at onset. However, the arterial flow is surprisingly well maintained on the cerebral surface. We investigated cortical microcirculatory changes in the super acute phase of SAH using two-photon laser scanning microscopy (TPLSM). Methods SAH was induced at the skull base in 10 mice using a prone endovascular perforation model. Before SAH, and 1, 2, 5, 10, 20, 30 and 60 min after SAH, the cortical microcirculation was observed with TPLSM through a cranial window. Diameters of penetrating and precapillary arterioles were measured and red blood cell (RBC) velocities in precapillary arterioles were analyzed using a line-scan method after administration of Q-dot 655 nanocrystals. Results One minute after SAH, RBC velocity and flow in precapillary arterioles drastically decreased to",
keywords = "Ischemia, Microcirculation, Neurovascular function, Subarachnoid hemorrhage, Vasodilation",
author = "Mami Ishikawa and Mayumi Kajimura and Takayuki Morikawa and Kosuke Tsukada and Toshiyuki Tsuji and Gen Kusaka and Yuichi Tanaka and Makoto Suematsu",
year = "2016",
month = "9",
day = "15",
doi = "10.1016/j.jns.2016.06.067",
language = "English",
volume = "368",
pages = "326--333",
journal = "Journal of the Neurological Sciences",
issn = "0022-510X",
publisher = "Elsevier",

}

TY - JOUR

T1 - Cortical microcirculatory disturbance in the super acute phase of subarachnoid hemorrhage - In vivo analysis using two-photon laser scanning microscopy

AU - Ishikawa, Mami

AU - Kajimura, Mayumi

AU - Morikawa, Takayuki

AU - Tsukada, Kosuke

AU - Tsuji, Toshiyuki

AU - Kusaka, Gen

AU - Tanaka, Yuichi

AU - Suematsu, Makoto

PY - 2016/9/15

Y1 - 2016/9/15

N2 - Objective Subarachnoid hemorrhage (SAH) causes cerebral ischemia and drastically worsens the clinical status at onset. However, the arterial flow is surprisingly well maintained on the cerebral surface. We investigated cortical microcirculatory changes in the super acute phase of SAH using two-photon laser scanning microscopy (TPLSM). Methods SAH was induced at the skull base in 10 mice using a prone endovascular perforation model. Before SAH, and 1, 2, 5, 10, 20, 30 and 60 min after SAH, the cortical microcirculation was observed with TPLSM through a cranial window. Diameters of penetrating and precapillary arterioles were measured and red blood cell (RBC) velocities in precapillary arterioles were analyzed using a line-scan method after administration of Q-dot 655 nanocrystals. Results One minute after SAH, RBC velocity and flow in precapillary arterioles drastically decreased to

AB - Objective Subarachnoid hemorrhage (SAH) causes cerebral ischemia and drastically worsens the clinical status at onset. However, the arterial flow is surprisingly well maintained on the cerebral surface. We investigated cortical microcirculatory changes in the super acute phase of SAH using two-photon laser scanning microscopy (TPLSM). Methods SAH was induced at the skull base in 10 mice using a prone endovascular perforation model. Before SAH, and 1, 2, 5, 10, 20, 30 and 60 min after SAH, the cortical microcirculation was observed with TPLSM through a cranial window. Diameters of penetrating and precapillary arterioles were measured and red blood cell (RBC) velocities in precapillary arterioles were analyzed using a line-scan method after administration of Q-dot 655 nanocrystals. Results One minute after SAH, RBC velocity and flow in precapillary arterioles drastically decreased to

KW - Ischemia

KW - Microcirculation

KW - Neurovascular function

KW - Subarachnoid hemorrhage

KW - Vasodilation

UR - http://www.scopus.com/inward/record.url?scp=84979523434&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84979523434&partnerID=8YFLogxK

U2 - 10.1016/j.jns.2016.06.067

DO - 10.1016/j.jns.2016.06.067

M3 - Article

C2 - 27538658

AN - SCOPUS:84979523434

VL - 368

SP - 326

EP - 333

JO - Journal of the Neurological Sciences

JF - Journal of the Neurological Sciences

SN - 0022-510X

ER -