Cortical spreading depression (CSD)-induced tolerance to transient focal cerebral ischemia in halothane anesthetized rats is affected by anesthetic level but not ATP-sensitive potassium channels

Takashi Horiguchi, Bela Kis, Nishadi Rajapakse, Katsuyoshi Shimizu, David W. Busija

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We investigated the participation of ATP-sensitive potassium (K ATP) channels, adenosine A1 receptors, and the effects of different levels of halothane anesthesia in the development of CSD-induced ischemic tolerance. To elicit CSD, 0.5 M KCl was applied for 2 h to the right hemisphere of halothane anesthetized male Wistar rats. The inhalation concentration of halothane during CSD was maintained at 0.5% (n = 8), 1.0% (n = 8), or 2.0% (n = 8). For control animals, saline was applied instead of KCl (n = 8). To inhibit KATP channels or adenosine A1 receptors, glibenclamide (0.1 mg/kg icv; n = 8), 5-hydroxydeconaoate (5-HD; 100 mg/kg ip; n = 12), or 8-Cyclopentyl-1, 3-dipropylxanthine (DPCPX) (1.0 mg/kg ip; n = 8) was applied before preconditioning during 1.0% halothane anesthesia. Temporary occlusion (120 min) of the right middle cerebral artery was induced 4 days after preconditioning and the infarct volume was measured. Preconditioning elicited under 1.0% halothane reduced cortical infarct volume from 277 ± 15 mm3 in the control group to 159 ± 14 mm3 in the CSD group (mean ± SEM, P < 0.05). In contrast, CSD induced during inhalation of 0.5% or 2.0% halothane did not confer ischemic tolerance. The reduction in infarct area with CSD during inhalation of 1% halothane was not changed in animals treated with glibenclamide or 5-HD or DPCPX. These results uncover a crucial role of halothane level but not of KATP channels or adenosine A1 receptors in the preconditioning effects of CSD.

Original languageEnglish
Pages (from-to)127-133
Number of pages7
JournalBrain Research
Volume1062
Issue number1-2
DOIs
Publication statusPublished - 2005 Nov 16
Externally publishedYes

Fingerprint

Cortical Spreading Depression
KATP Channels
Transient Ischemic Attack
Halothane
Anesthetics
Adenosine A1 Receptors
Inhalation
Glyburide
Anesthesia
Middle Cerebral Artery
Wistar Rats
Adenosine Triphosphate
Control Groups

Keywords

  • Adenosine receptor
  • ATP-sensitive potassium channel
  • Cerebral cortex
  • Nitric oxide
  • Preconditioning
  • Stroke

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cortical spreading depression (CSD)-induced tolerance to transient focal cerebral ischemia in halothane anesthetized rats is affected by anesthetic level but not ATP-sensitive potassium channels. / Horiguchi, Takashi; Kis, Bela; Rajapakse, Nishadi; Shimizu, Katsuyoshi; Busija, David W.

In: Brain Research, Vol. 1062, No. 1-2, 16.11.2005, p. 127-133.

Research output: Contribution to journalArticle

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AU - Shimizu, Katsuyoshi

AU - Busija, David W.

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