Corticosterone increases Na+-K+-ATPase activity in rat cortisal collecting ducts with inhibition of 11β-hydroxysteroid dehydrogenase

H. Tsuganezawa, M. Hayashi, Y. Fujii, Y. Yamaji, M. Iyori, T. Saruta

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

To examine a physiological role of 11β-hydroxysteroid dehydrogenase (11OHSD) in the aldosterone target tissues, we measured Na+-K+-ATPase activity in the cortical collecting ducts (CCD) from adrenalectomized rats, which were treated with a physiological dose of corticosterone and/or carbenoxolone (an inhibitor of 11OHSD). The Na+-K+-ATPase activity in adrenalectomized rats was not significantly changed by either corticosterone alone or carbenoxolone alone, whereas its activity showed a significant increase only in the rats that received both corticosterone and carbenoxolone. In these rats, the plasma concentration of corticosterone was within the physiological range (10-6M) and the plasma carbenoxolone concentration was about 10-7M. Furthermore, the direct effect of carbenosolone was examined in the microdissected CCB because it has been reported that carbenoxolone per se had an affinity for the aldosterone receptor. Na+K+-ATPase activity in the microdissected CCD was increased in a dose-dependent manner after a 3-hour incubation with carbenoxolone, and this effect was completely inhibited by canrenoic acid (an aldosterone antagonist), However, the minimal carbenoxolone concentration exerting a stimulatory effect was 10-6M, which is about 10 times higher than the plasma concentration of carbenoxolone in the in vivo treated rats. These results indicate that an inhibition of 11OHSD but not a direct action of carbenoxolone induces an increase in Na+-K+-ATPase activity, which is a well-known aldosterone effect in the CCD.

Original languageEnglish
Pages (from-to)66-72
Number of pages7
JournalRenal Physiology and Biochemistry
Volume18
Issue number2
Publication statusPublished - 1995

Fingerprint

Carbenoxolone
11-beta-Hydroxysteroid Dehydrogenases
Corticosterone
Aldosterone
Canrenoic Acid
Mineralocorticoid Receptor Antagonists
sodium-translocating ATPase
Mineralocorticoid Receptors

Keywords

  • 11β-hydroxysteroid dehydrogenase
  • Carbenoxolone
  • Collecting duct
  • Corticosterone
  • Na-K-ATPase

ASJC Scopus subject areas

  • Physiology
  • Nephrology

Cite this

Corticosterone increases Na+-K+-ATPase activity in rat cortisal collecting ducts with inhibition of 11β-hydroxysteroid dehydrogenase. / Tsuganezawa, H.; Hayashi, M.; Fujii, Y.; Yamaji, Y.; Iyori, M.; Saruta, T.

In: Renal Physiology and Biochemistry, Vol. 18, No. 2, 1995, p. 66-72.

Research output: Contribution to journalArticle

Tsuganezawa, H. ; Hayashi, M. ; Fujii, Y. ; Yamaji, Y. ; Iyori, M. ; Saruta, T. / Corticosterone increases Na+-K+-ATPase activity in rat cortisal collecting ducts with inhibition of 11β-hydroxysteroid dehydrogenase. In: Renal Physiology and Biochemistry. 1995 ; Vol. 18, No. 2. pp. 66-72.
@article{b32c154d870a4bf795a2e2ef18fbea02,
title = "Corticosterone increases Na+-K+-ATPase activity in rat cortisal collecting ducts with inhibition of 11β-hydroxysteroid dehydrogenase",
abstract = "To examine a physiological role of 11β-hydroxysteroid dehydrogenase (11OHSD) in the aldosterone target tissues, we measured Na+-K+-ATPase activity in the cortical collecting ducts (CCD) from adrenalectomized rats, which were treated with a physiological dose of corticosterone and/or carbenoxolone (an inhibitor of 11OHSD). The Na+-K+-ATPase activity in adrenalectomized rats was not significantly changed by either corticosterone alone or carbenoxolone alone, whereas its activity showed a significant increase only in the rats that received both corticosterone and carbenoxolone. In these rats, the plasma concentration of corticosterone was within the physiological range (10-6M) and the plasma carbenoxolone concentration was about 10-7M. Furthermore, the direct effect of carbenosolone was examined in the microdissected CCB because it has been reported that carbenoxolone per se had an affinity for the aldosterone receptor. Na+K+-ATPase activity in the microdissected CCD was increased in a dose-dependent manner after a 3-hour incubation with carbenoxolone, and this effect was completely inhibited by canrenoic acid (an aldosterone antagonist), However, the minimal carbenoxolone concentration exerting a stimulatory effect was 10-6M, which is about 10 times higher than the plasma concentration of carbenoxolone in the in vivo treated rats. These results indicate that an inhibition of 11OHSD but not a direct action of carbenoxolone induces an increase in Na+-K+-ATPase activity, which is a well-known aldosterone effect in the CCD.",
keywords = "11β-hydroxysteroid dehydrogenase, Carbenoxolone, Collecting duct, Corticosterone, Na-K-ATPase",
author = "H. Tsuganezawa and M. Hayashi and Y. Fujii and Y. Yamaji and M. Iyori and T. Saruta",
year = "1995",
language = "English",
volume = "18",
pages = "66--72",
journal = "Kidney and Blood Pressure Research",
issn = "1420-4096",
publisher = "S. Karger AG",
number = "2",

}

TY - JOUR

T1 - Corticosterone increases Na+-K+-ATPase activity in rat cortisal collecting ducts with inhibition of 11β-hydroxysteroid dehydrogenase

AU - Tsuganezawa, H.

AU - Hayashi, M.

AU - Fujii, Y.

AU - Yamaji, Y.

AU - Iyori, M.

AU - Saruta, T.

PY - 1995

Y1 - 1995

N2 - To examine a physiological role of 11β-hydroxysteroid dehydrogenase (11OHSD) in the aldosterone target tissues, we measured Na+-K+-ATPase activity in the cortical collecting ducts (CCD) from adrenalectomized rats, which were treated with a physiological dose of corticosterone and/or carbenoxolone (an inhibitor of 11OHSD). The Na+-K+-ATPase activity in adrenalectomized rats was not significantly changed by either corticosterone alone or carbenoxolone alone, whereas its activity showed a significant increase only in the rats that received both corticosterone and carbenoxolone. In these rats, the plasma concentration of corticosterone was within the physiological range (10-6M) and the plasma carbenoxolone concentration was about 10-7M. Furthermore, the direct effect of carbenosolone was examined in the microdissected CCB because it has been reported that carbenoxolone per se had an affinity for the aldosterone receptor. Na+K+-ATPase activity in the microdissected CCD was increased in a dose-dependent manner after a 3-hour incubation with carbenoxolone, and this effect was completely inhibited by canrenoic acid (an aldosterone antagonist), However, the minimal carbenoxolone concentration exerting a stimulatory effect was 10-6M, which is about 10 times higher than the plasma concentration of carbenoxolone in the in vivo treated rats. These results indicate that an inhibition of 11OHSD but not a direct action of carbenoxolone induces an increase in Na+-K+-ATPase activity, which is a well-known aldosterone effect in the CCD.

AB - To examine a physiological role of 11β-hydroxysteroid dehydrogenase (11OHSD) in the aldosterone target tissues, we measured Na+-K+-ATPase activity in the cortical collecting ducts (CCD) from adrenalectomized rats, which were treated with a physiological dose of corticosterone and/or carbenoxolone (an inhibitor of 11OHSD). The Na+-K+-ATPase activity in adrenalectomized rats was not significantly changed by either corticosterone alone or carbenoxolone alone, whereas its activity showed a significant increase only in the rats that received both corticosterone and carbenoxolone. In these rats, the plasma concentration of corticosterone was within the physiological range (10-6M) and the plasma carbenoxolone concentration was about 10-7M. Furthermore, the direct effect of carbenosolone was examined in the microdissected CCB because it has been reported that carbenoxolone per se had an affinity for the aldosterone receptor. Na+K+-ATPase activity in the microdissected CCD was increased in a dose-dependent manner after a 3-hour incubation with carbenoxolone, and this effect was completely inhibited by canrenoic acid (an aldosterone antagonist), However, the minimal carbenoxolone concentration exerting a stimulatory effect was 10-6M, which is about 10 times higher than the plasma concentration of carbenoxolone in the in vivo treated rats. These results indicate that an inhibition of 11OHSD but not a direct action of carbenoxolone induces an increase in Na+-K+-ATPase activity, which is a well-known aldosterone effect in the CCD.

KW - 11β-hydroxysteroid dehydrogenase

KW - Carbenoxolone

KW - Collecting duct

KW - Corticosterone

KW - Na-K-ATPase

UR - http://www.scopus.com/inward/record.url?scp=0028904478&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028904478&partnerID=8YFLogxK

M3 - Article

C2 - 7539535

AN - SCOPUS:0028904478

VL - 18

SP - 66

EP - 72

JO - Kidney and Blood Pressure Research

JF - Kidney and Blood Pressure Research

SN - 1420-4096

IS - 2

ER -