Cosecretion of peptides derived from gamma-human atrial natriuretic polypeptide in normal volunteers and patients with essential hypertension and adrenal disorders

Akira Sugawara, Kazuwa Nakao, Hiroshi Itoh, Takayuki Yamada, Yoshihiko Saito, Masashi Mukoyama, Hiroshi Arai, Kiminori Hosoda, Gotaro Shirakami, Narito Morii, Shozo Shiono, Hiroo Imura

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Using two radio-immunoassays for N-terminal and C-terminal fragments of human atrial natriuretic polypeptide (ANP) precursor, γ-hANP [human atrial natriuretic factor- (1-126)], that is γ -hANP(1-25) [human atrial natriuretic factor-(1-25)] and α-hANP [human atrial natriuretic factor-(99-126)], we studied the secretion of γ -hANP-derived peptides into circulation from the heart in normal subjects and patients with essential hypertension and adrenal disorders. Volume expansion with 2 litres physiological saline increased plasma γ -hANP(1-25)-like immunoreactivity concomitantly with plasma α - hANP-like immunoreactivity in normal subjects. Infusion of angiotensin II (20 ng/kg per min) or noradrenaline (200 ng/kg per min) also caused a parallel increase in plasma γ -hANP(1-25)-like and α -hANP-like immunoreactivity. Plasma γ -hANP(1-25)-like immunoreactivity levels were changed together with α -hANP-like immunoreactivity in patients with essential hypertension and adrenal disorders. These results indicate that γ -hANP-derived peptides, α -hANP and the 10-k N- terminal fragment of γ -hANP (N-peptide) are cosecreted from the heart and that the simultaneous measurement of N-peptide and α -hANP serves as an indicator of the cardiac endocrine function. The significance of N-peptide as a hormone must await further clarification.

Original languageEnglish
Pages (from-to)S327-S329
JournalJournal of Hypertension, Supplement
Volume6
Issue number4
Publication statusPublished - 1988
Externally publishedYes

Fingerprint

Healthy Volunteers
Peptides
Atrial Natriuretic Factor
Radio
Immunoassay
Angiotensin II
Essential Hypertension
Norepinephrine
Hormones

Keywords

  • Adrenal disorders
  • Cosecretion
  • Essential hypertension
  • N-terminal fragment of γ-human atrial natriuretic polypeptide (N-peptide)
  • α-human atrial natriuretic polypeptide
  • γ-human atrial natriuretic polypeptide

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Internal Medicine
  • Endocrinology

Cite this

Cosecretion of peptides derived from gamma-human atrial natriuretic polypeptide in normal volunteers and patients with essential hypertension and adrenal disorders. / Sugawara, Akira; Nakao, Kazuwa; Itoh, Hiroshi; Yamada, Takayuki; Saito, Yoshihiko; Mukoyama, Masashi; Arai, Hiroshi; Hosoda, Kiminori; Shirakami, Gotaro; Morii, Narito; Shiono, Shozo; Imura, Hiroo.

In: Journal of Hypertension, Supplement, Vol. 6, No. 4, 1988, p. S327-S329.

Research output: Contribution to journalArticle

Sugawara, A, Nakao, K, Itoh, H, Yamada, T, Saito, Y, Mukoyama, M, Arai, H, Hosoda, K, Shirakami, G, Morii, N, Shiono, S & Imura, H 1988, 'Cosecretion of peptides derived from gamma-human atrial natriuretic polypeptide in normal volunteers and patients with essential hypertension and adrenal disorders', Journal of Hypertension, Supplement, vol. 6, no. 4, pp. S327-S329.
Sugawara, Akira ; Nakao, Kazuwa ; Itoh, Hiroshi ; Yamada, Takayuki ; Saito, Yoshihiko ; Mukoyama, Masashi ; Arai, Hiroshi ; Hosoda, Kiminori ; Shirakami, Gotaro ; Morii, Narito ; Shiono, Shozo ; Imura, Hiroo. / Cosecretion of peptides derived from gamma-human atrial natriuretic polypeptide in normal volunteers and patients with essential hypertension and adrenal disorders. In: Journal of Hypertension, Supplement. 1988 ; Vol. 6, No. 4. pp. S327-S329.
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T1 - Cosecretion of peptides derived from gamma-human atrial natriuretic polypeptide in normal volunteers and patients with essential hypertension and adrenal disorders

AU - Sugawara, Akira

AU - Nakao, Kazuwa

AU - Itoh, Hiroshi

AU - Yamada, Takayuki

AU - Saito, Yoshihiko

AU - Mukoyama, Masashi

AU - Arai, Hiroshi

AU - Hosoda, Kiminori

AU - Shirakami, Gotaro

AU - Morii, Narito

AU - Shiono, Shozo

AU - Imura, Hiroo

PY - 1988

Y1 - 1988

N2 - Using two radio-immunoassays for N-terminal and C-terminal fragments of human atrial natriuretic polypeptide (ANP) precursor, γ-hANP [human atrial natriuretic factor- (1-126)], that is γ -hANP(1-25) [human atrial natriuretic factor-(1-25)] and α-hANP [human atrial natriuretic factor-(99-126)], we studied the secretion of γ -hANP-derived peptides into circulation from the heart in normal subjects and patients with essential hypertension and adrenal disorders. Volume expansion with 2 litres physiological saline increased plasma γ -hANP(1-25)-like immunoreactivity concomitantly with plasma α - hANP-like immunoreactivity in normal subjects. Infusion of angiotensin II (20 ng/kg per min) or noradrenaline (200 ng/kg per min) also caused a parallel increase in plasma γ -hANP(1-25)-like and α -hANP-like immunoreactivity. Plasma γ -hANP(1-25)-like immunoreactivity levels were changed together with α -hANP-like immunoreactivity in patients with essential hypertension and adrenal disorders. These results indicate that γ -hANP-derived peptides, α -hANP and the 10-k N- terminal fragment of γ -hANP (N-peptide) are cosecreted from the heart and that the simultaneous measurement of N-peptide and α -hANP serves as an indicator of the cardiac endocrine function. The significance of N-peptide as a hormone must await further clarification.

AB - Using two radio-immunoassays for N-terminal and C-terminal fragments of human atrial natriuretic polypeptide (ANP) precursor, γ-hANP [human atrial natriuretic factor- (1-126)], that is γ -hANP(1-25) [human atrial natriuretic factor-(1-25)] and α-hANP [human atrial natriuretic factor-(99-126)], we studied the secretion of γ -hANP-derived peptides into circulation from the heart in normal subjects and patients with essential hypertension and adrenal disorders. Volume expansion with 2 litres physiological saline increased plasma γ -hANP(1-25)-like immunoreactivity concomitantly with plasma α - hANP-like immunoreactivity in normal subjects. Infusion of angiotensin II (20 ng/kg per min) or noradrenaline (200 ng/kg per min) also caused a parallel increase in plasma γ -hANP(1-25)-like and α -hANP-like immunoreactivity. Plasma γ -hANP(1-25)-like immunoreactivity levels were changed together with α -hANP-like immunoreactivity in patients with essential hypertension and adrenal disorders. These results indicate that γ -hANP-derived peptides, α -hANP and the 10-k N- terminal fragment of γ -hANP (N-peptide) are cosecreted from the heart and that the simultaneous measurement of N-peptide and α -hANP serves as an indicator of the cardiac endocrine function. The significance of N-peptide as a hormone must await further clarification.

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KW - Essential hypertension

KW - N-terminal fragment of γ-human atrial natriuretic polypeptide (N-peptide)

KW - α-human atrial natriuretic polypeptide

KW - γ-human atrial natriuretic polypeptide

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JO - Journal of Hypertension, Supplement

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