Cost-effective culture of human induced pluripotent stem cells using UV/ozone-modified culture plastics with reduction of cell-adhesive matrix coating

Kohei Kasai, Shugo Tohyama, Hayato Suzuki, Sho Tanosaki, Keiichi Fukuda, Jun Fujita, Shogo Miyata

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Human induced pluripotent stem cells (hiPSCs) are considered to be one of the most promising cell resources for regenerative medicine. HiPSCs usually maintain their pluripotency when they are cultured on feeder cell layers or are attached to a cell-adhesive extracellular matrix. In this study, we developed a culture system based on UV/ozone modification for conventional cell culture plastics to generate a suitable surface condition for hiPSCs. Time of flight secondary ion mass spectrometry (ToF-SIMS) was carried out to elucidate the relationship between hiPSC adhesion and UV/ozone irradiation-induced changes to surface chemistry of cell culture plastics. Cell culture plastics with modified surfaces enabled growth of a feeder-free hiPSC culture with markedly reduced cell-adhesive matrix coating. Our cell culture system using UV/ozone-modified cell culture plastics may produce clinically relevant hiPSCs at low costs, and can be easily scaled up in culture systems to produce a large number of hiPSCs.

Original languageEnglish
Article number110788
JournalMaterials Science and Engineering C
Volume111
DOIs
Publication statusPublished - 2020 Jun

Keywords

  • Cell adhesive matrix
  • Cell culture plastics
  • Human induced pluripotent stem cell
  • Surface chemistry
  • UV/ozone surface modification

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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