Could frameshift mutations in the frxA and rdxA genes of Helicobacter pylori be a marker for metronidazole resistance?

Tatsuhiro Masaoka, Hidekazu Suzuki, K. Kurabayashi, Y. Nomoto, T. Nishizawa, M. Mori, T. Hibi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Although resistance of Helicobacter pylori to metronidazole had been reported to be associated with mutations in the rdxA or the frxA gene, recent studies have indicated that they may contribute little to metronidazole resistance. Aim: To clarify the roles of these genes in metronidazole resistance, we examined them in strains that were not eradicated by first-line eradication therapy. Patients and methods: A total of 132 patients (92 males, 40 females, average age: 53.8 years old) who underwent upper gastrointestinal endoscopy after unsuccessful first-line H. pylori eradication were enrolled. Antibiotic susceptibility to metronidazole was investigated using the agar dilution method. The H. pylorirdxA and frxA genes were then sequenced. Results: In the metronidazole-resistant strains, the sensitivity of detection of frameshift mutations in the rdxA gene was 44%. A significant association between the presence of frameshift mutations in the rdxA gene and resistance to metronidazole was observed. No significant association between frameshift mutations in the frxA gene and resistance to metronidazole was observed. Conclusion: Mutation in the rdxA gene was sufficient, but not necessary to confer resistance to metronidazole in H. pylori. Mutation in the frxA gene was not necessary for resistance to metronidazole in H. pylori.

Original languageEnglish
Pages (from-to)81-87
Number of pages7
JournalAlimentary Pharmacology and Therapeutics
Volume24
Issue numberSUPPL.4
DOIs
Publication statusPublished - 2006 Dec

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Frameshift Mutation
Metronidazole
Helicobacter pylori
Genes
Mutation
Gastrointestinal Endoscopy
Agar
Anti-Bacterial Agents

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Could frameshift mutations in the frxA and rdxA genes of Helicobacter pylori be a marker for metronidazole resistance? / Masaoka, Tatsuhiro; Suzuki, Hidekazu; Kurabayashi, K.; Nomoto, Y.; Nishizawa, T.; Mori, M.; Hibi, T.

In: Alimentary Pharmacology and Therapeutics, Vol. 24, No. SUPPL.4, 12.2006, p. 81-87.

Research output: Contribution to journalArticle

Masaoka, Tatsuhiro ; Suzuki, Hidekazu ; Kurabayashi, K. ; Nomoto, Y. ; Nishizawa, T. ; Mori, M. ; Hibi, T. / Could frameshift mutations in the frxA and rdxA genes of Helicobacter pylori be a marker for metronidazole resistance?. In: Alimentary Pharmacology and Therapeutics. 2006 ; Vol. 24, No. SUPPL.4. pp. 81-87.
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abstract = "Background: Although resistance of Helicobacter pylori to metronidazole had been reported to be associated with mutations in the rdxA or the frxA gene, recent studies have indicated that they may contribute little to metronidazole resistance. Aim: To clarify the roles of these genes in metronidazole resistance, we examined them in strains that were not eradicated by first-line eradication therapy. Patients and methods: A total of 132 patients (92 males, 40 females, average age: 53.8 years old) who underwent upper gastrointestinal endoscopy after unsuccessful first-line H. pylori eradication were enrolled. Antibiotic susceptibility to metronidazole was investigated using the agar dilution method. The H. pylorirdxA and frxA genes were then sequenced. Results: In the metronidazole-resistant strains, the sensitivity of detection of frameshift mutations in the rdxA gene was 44{\%}. A significant association between the presence of frameshift mutations in the rdxA gene and resistance to metronidazole was observed. No significant association between frameshift mutations in the frxA gene and resistance to metronidazole was observed. Conclusion: Mutation in the rdxA gene was sufficient, but not necessary to confer resistance to metronidazole in H. pylori. Mutation in the frxA gene was not necessary for resistance to metronidazole in H. pylori.",
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T1 - Could frameshift mutations in the frxA and rdxA genes of Helicobacter pylori be a marker for metronidazole resistance?

AU - Masaoka, Tatsuhiro

AU - Suzuki, Hidekazu

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AU - Nomoto, Y.

AU - Nishizawa, T.

AU - Mori, M.

AU - Hibi, T.

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N2 - Background: Although resistance of Helicobacter pylori to metronidazole had been reported to be associated with mutations in the rdxA or the frxA gene, recent studies have indicated that they may contribute little to metronidazole resistance. Aim: To clarify the roles of these genes in metronidazole resistance, we examined them in strains that were not eradicated by first-line eradication therapy. Patients and methods: A total of 132 patients (92 males, 40 females, average age: 53.8 years old) who underwent upper gastrointestinal endoscopy after unsuccessful first-line H. pylori eradication were enrolled. Antibiotic susceptibility to metronidazole was investigated using the agar dilution method. The H. pylorirdxA and frxA genes were then sequenced. Results: In the metronidazole-resistant strains, the sensitivity of detection of frameshift mutations in the rdxA gene was 44%. A significant association between the presence of frameshift mutations in the rdxA gene and resistance to metronidazole was observed. No significant association between frameshift mutations in the frxA gene and resistance to metronidazole was observed. Conclusion: Mutation in the rdxA gene was sufficient, but not necessary to confer resistance to metronidazole in H. pylori. Mutation in the frxA gene was not necessary for resistance to metronidazole in H. pylori.

AB - Background: Although resistance of Helicobacter pylori to metronidazole had been reported to be associated with mutations in the rdxA or the frxA gene, recent studies have indicated that they may contribute little to metronidazole resistance. Aim: To clarify the roles of these genes in metronidazole resistance, we examined them in strains that were not eradicated by first-line eradication therapy. Patients and methods: A total of 132 patients (92 males, 40 females, average age: 53.8 years old) who underwent upper gastrointestinal endoscopy after unsuccessful first-line H. pylori eradication were enrolled. Antibiotic susceptibility to metronidazole was investigated using the agar dilution method. The H. pylorirdxA and frxA genes were then sequenced. Results: In the metronidazole-resistant strains, the sensitivity of detection of frameshift mutations in the rdxA gene was 44%. A significant association between the presence of frameshift mutations in the rdxA gene and resistance to metronidazole was observed. No significant association between frameshift mutations in the frxA gene and resistance to metronidazole was observed. Conclusion: Mutation in the rdxA gene was sufficient, but not necessary to confer resistance to metronidazole in H. pylori. Mutation in the frxA gene was not necessary for resistance to metronidazole in H. pylori.

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