Coupling of σG Activation to Completion of Engulfment during Sporulation of Bacillus subtilis Survives Large Perturbations to DNA Translocation and Replication

Genevieve Regan, Mitsuhiro Itaya, Patrick J. Piggot

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Spore formation in Bacillus subtilis is characterized by activation of RNA polymerase sigma factors, including the late-expressed σG. During spore formation an asymmetric division occurs, yielding the smaller prespore and the largermother cell. At division, only 30%of the chromosome is in the prespore, and the rest is then translocated into the prespore. Following completion of en-gulfment of the prespore by themother cell, σG is activated in the prespore. Here we tested the link between engulfment and σG activation by perturbing DNA translocation and replication, which are completed before engulfment. One approach was to have large DNA insertions in the chromosome; the second was to have an impaired DNA translocase; the third was to use a strain in which the site of termination of chromosome replication was relocated. Insertion of 2.3Mb of Synechocystis DNA into the B. subtilis genome had the largest effect, delaying engulfment by at least 90min. Chromosome translocation was also delayed and was completed shortly before the completion of engulfment. Despite the delay, σG became active only after the completion of engulfment. All results are consistent with a strong link between completion of engulfment and σG activation. They support a link between completion of chromosome translocation and completion of engulfment.

Original languageEnglish
Pages (from-to)6264-6271
Number of pages8
JournalJournal of Bacteriology
Volume194
Issue number22
DOIs
Publication statusPublished - 2012 Nov

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Bacillus subtilis
DNA Replication
Chromosomes
Spores
DNA
Synechocystis
Sigma Factor
Gastrin-Secreting Cells
DNA-Directed RNA Polymerases
Genome

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Coupling of σG Activation to Completion of Engulfment during Sporulation of Bacillus subtilis Survives Large Perturbations to DNA Translocation and Replication. / Regan, Genevieve; Itaya, Mitsuhiro; Piggot, Patrick J.

In: Journal of Bacteriology, Vol. 194, No. 22, 11.2012, p. 6264-6271.

Research output: Contribution to journalArticle

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