Covalent change of major intrinsic polypeptide (MIP26K) of lens membrane during human senile cataractogenesis

L. Takemoto, M. Takehana

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Polyclonal antisera have been made against synthetic peptides corresponding to the C-terminal octapeptide and N-terminal nonapeptide of bovine MIP26K. Western blot analysis demonstrated significant binding of the C-terminal antiserum to MIP26K of both normal and cataractous human lens. In contrast, the N-terminal antiserum bound to MIP26K of normal lenses, but failed to bind to MIP26K of 7 out of 10 cataractous lenses studied. These results demonstrate for the first time, a covalent change in MIP26K during human cataractogenesis, and strongly suggest that the location of this change is in the N-terminal region of the polypeptide.

Original languageEnglish
Pages (from-to)965-971
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume135
Issue number3
DOIs
Publication statusPublished - 1986 Mar 28

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Lenses
Immune Sera
Membranes
Peptides
Western Blotting

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Covalent change of major intrinsic polypeptide (MIP26K) of lens membrane during human senile cataractogenesis. / Takemoto, L.; Takehana, M.

In: Biochemical and Biophysical Research Communications, Vol. 135, No. 3, 28.03.1986, p. 965-971.

Research output: Contribution to journalArticle

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