TY - JOUR
T1 - Covalent change of major intrinsic polypeptide (MIP26K) of lens membrane during human senile cataractogenesis
AU - Takemoto, L.
AU - Takehana, M.
PY - 1986/3/28
Y1 - 1986/3/28
N2 - Polyclonal antisera have been made against synthetic peptides corresponding to the C-terminal octapeptide and N-terminal nonapeptide of bovine MIP26K. Western blot analysis demonstrated significant binding of the C-terminal antiserum to MIP26K of both normal and cataractous human lens. In contrast, the N-terminal antiserum bound to MIP26K of normal lenses, but failed to bind to MIP26K of 7 out of 10 cataractous lenses studied. These results demonstrate for the first time, a covalent change in MIP26K during human cataractogenesis, and strongly suggest that the location of this change is in the N-terminal region of the polypeptide.
AB - Polyclonal antisera have been made against synthetic peptides corresponding to the C-terminal octapeptide and N-terminal nonapeptide of bovine MIP26K. Western blot analysis demonstrated significant binding of the C-terminal antiserum to MIP26K of both normal and cataractous human lens. In contrast, the N-terminal antiserum bound to MIP26K of normal lenses, but failed to bind to MIP26K of 7 out of 10 cataractous lenses studied. These results demonstrate for the first time, a covalent change in MIP26K during human cataractogenesis, and strongly suggest that the location of this change is in the N-terminal region of the polypeptide.
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U2 - 10.1016/0006-291X(86)91022-3
DO - 10.1016/0006-291X(86)91022-3
M3 - Article
C2 - 2421726
AN - SCOPUS:0022500721
SN - 0006-291X
VL - 135
SP - 965
EP - 971
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -