Covalent changes in MIP26K during aging of the human lens membrane

L. Takemoto, M. Takehana, J. Horwitz

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Rabbit antisera have been made to the C-terminal octapeptide of bovine MIP26K (residues 256-263) and to an octapeptide near the C-terminus (residues 252-259). Use of these antisera in Western blot analysis of membrane from young vs old human lenses, and from cortex vs nucleus of old human lenses, suggest age-related covalent changes in the C-terminus of MIP26K. These age-related changes in anti-256-263 and anti-252-259 sera can be mimicked by treatment of lens membrane with carboxypeptidase Y, suggesting the loss of 1-8 amino acid residues from the C-terminus of the 26,000 dalton form of MIP26K during aging of the normal human lens.

Original languageEnglish
Pages (from-to)443-446
Number of pages4
JournalInvestigative Ophthalmology and Visual Science
Volume27
Issue number3
Publication statusPublished - 1986

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Lenses
Membranes
Immune Sera
Cathepsin A
Western Blotting
Rabbits
Amino Acids
Serum

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Covalent changes in MIP26K during aging of the human lens membrane. / Takemoto, L.; Takehana, M.; Horwitz, J.

In: Investigative Ophthalmology and Visual Science, Vol. 27, No. 3, 1986, p. 443-446.

Research output: Contribution to journalArticle

Takemoto, L, Takehana, M & Horwitz, J 1986, 'Covalent changes in MIP26K during aging of the human lens membrane', Investigative Ophthalmology and Visual Science, vol. 27, no. 3, pp. 443-446.
Takemoto, L. ; Takehana, M. ; Horwitz, J. / Covalent changes in MIP26K during aging of the human lens membrane. In: Investigative Ophthalmology and Visual Science. 1986 ; Vol. 27, No. 3. pp. 443-446.
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