Abstract
Craniopharyngioma is a rare tumor occurring in the sellar region comprising 3% of all intracranial tumors. To elucidate the contribution of β-catenin gene mutation to tumorigenesis, we examined genetic alterations and expression of β-catenin in 10 cases of adamantinomatous and 6 cases of papillary craniopharyngiomas. β-Catenin gene mutations were found in all of the adamantinomatous and none of the papillary craniopharyngiomas. Immunohistochemically, all cases of adamantinomatous craniopharyngioma showed cytoplasmic and nuclear expression of β-catenin. In contrast, papillary craniopharyngiomas showed exclusively membranous expression. The results suggest that adamantinomatous- and papillary-type craniopharyngiomas are not only clinicopathologically, but also genetically, distinctive variants. Mutation of the β-catenin gene therefore seems to play an important role in the tumorigenesis of adamantinomatous craniopharyngioma. Among the adamantinomatous-type tumors, β-catenin-positive mesenchymal cells were observed in two cases. Microdissection-based mutational analysis revealed that these mesenchymal cells also harbor the same β-catenin gene mutations as those of epithelial cells, suggesting their tumorous nature. Thus, at least a subset of adamantinomatous craniopharyngioma is considered to be biphasic.
| Original language | English |
|---|---|
| Pages (from-to) | 1997-2001 |
| Number of pages | 5 |
| Journal | American Journal of Pathology |
| Volume | 161 |
| Issue number | 6 |
| Publication status | Published - 2002 Dec 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Pathology and Forensic Medicine
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Craniopharyngiomas of adamantinomatous type harbor β-Catenin gene mutations. / Sekine, Shigeki; Shibata, Tatsuhiro; Kokubu, Akiko; Morishita, Yukio; Noguchi, Masayuki; Nakanishi, Yukihiro; Sakamoto, Michiie; Hirohashi, Setsuo.
In: American Journal of Pathology, Vol. 161, No. 6, 01.12.2002, p. 1997-2001.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Craniopharyngiomas of adamantinomatous type harbor β-Catenin gene mutations
AU - Sekine, Shigeki
AU - Shibata, Tatsuhiro
AU - Kokubu, Akiko
AU - Morishita, Yukio
AU - Noguchi, Masayuki
AU - Nakanishi, Yukihiro
AU - Sakamoto, Michiie
AU - Hirohashi, Setsuo
PY - 2002/12/1
Y1 - 2002/12/1
N2 - Craniopharyngioma is a rare tumor occurring in the sellar region comprising 3% of all intracranial tumors. To elucidate the contribution of β-catenin gene mutation to tumorigenesis, we examined genetic alterations and expression of β-catenin in 10 cases of adamantinomatous and 6 cases of papillary craniopharyngiomas. β-Catenin gene mutations were found in all of the adamantinomatous and none of the papillary craniopharyngiomas. Immunohistochemically, all cases of adamantinomatous craniopharyngioma showed cytoplasmic and nuclear expression of β-catenin. In contrast, papillary craniopharyngiomas showed exclusively membranous expression. The results suggest that adamantinomatous- and papillary-type craniopharyngiomas are not only clinicopathologically, but also genetically, distinctive variants. Mutation of the β-catenin gene therefore seems to play an important role in the tumorigenesis of adamantinomatous craniopharyngioma. Among the adamantinomatous-type tumors, β-catenin-positive mesenchymal cells were observed in two cases. Microdissection-based mutational analysis revealed that these mesenchymal cells also harbor the same β-catenin gene mutations as those of epithelial cells, suggesting their tumorous nature. Thus, at least a subset of adamantinomatous craniopharyngioma is considered to be biphasic.
AB - Craniopharyngioma is a rare tumor occurring in the sellar region comprising 3% of all intracranial tumors. To elucidate the contribution of β-catenin gene mutation to tumorigenesis, we examined genetic alterations and expression of β-catenin in 10 cases of adamantinomatous and 6 cases of papillary craniopharyngiomas. β-Catenin gene mutations were found in all of the adamantinomatous and none of the papillary craniopharyngiomas. Immunohistochemically, all cases of adamantinomatous craniopharyngioma showed cytoplasmic and nuclear expression of β-catenin. In contrast, papillary craniopharyngiomas showed exclusively membranous expression. The results suggest that adamantinomatous- and papillary-type craniopharyngiomas are not only clinicopathologically, but also genetically, distinctive variants. Mutation of the β-catenin gene therefore seems to play an important role in the tumorigenesis of adamantinomatous craniopharyngioma. Among the adamantinomatous-type tumors, β-catenin-positive mesenchymal cells were observed in two cases. Microdissection-based mutational analysis revealed that these mesenchymal cells also harbor the same β-catenin gene mutations as those of epithelial cells, suggesting their tumorous nature. Thus, at least a subset of adamantinomatous craniopharyngioma is considered to be biphasic.
UR - http://www.scopus.com/inward/record.url?scp=0036898018&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036898018&partnerID=8YFLogxK
M3 - Article
C2 - 12466115
AN - SCOPUS:0036898018
VL - 161
SP - 1997
EP - 2001
JO - American Journal of Pathology
JF - American Journal of Pathology
SN - 0002-9440
IS - 6
ER -